Novel Insights into the Therapeutic Effect of Amentoflavone Against Aeromonas hydrophila Infection by Blocking the Activity of Aerolysin DOI Open Access
Jing Dong, Shengping Li, Shun Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2370 - 2370

Published: March 6, 2025

Aeromonas hydrophila (A. hydrophila) is an opportunistic and foodborne pathogen widely spread in the environments, particularly aquatic environments. Diseases caused by A. freshwater aquaculture result huge economic losses every year. The increasing emergence of antibiotic resistance has limited application antibiotics aquaculture. Aerolysin (AerA), main virulence factor produced hydrophila, been identified as a promising target for developing drugs controlling infection. Here, we found that natural compound amentoflavone (AMF) with MIC 512 μg/mL against could dose-dependently reduce hemolysis AerA, ranging from 0.5 to 4 μg/mL. Molecular docking dynamics simulation results predicted AMF directly bind domain 3 AerA via Pro333 Trp375 residues. Then, binding sites were confirmed fluorescence quenching assays. heptamer formation demonstrated affect oligomers loss pore-forming activity. Cell viability assay showed after treatment significantly AerA-mediated cytotoxicity. Moreover, experimental therapeutics channel catfish infected then administered 20 mg/kg at intervals 12 h days increase survival rate 35% compared positive control 10-day observation. These findings provided novel approach anti-infective candidate infection

Language: Английский

Novel Insights into the Therapeutic Effect of Amentoflavone Against Aeromonas hydrophila Infection by Blocking the Activity of Aerolysin DOI Open Access
Jing Dong, Shengping Li, Shun Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2370 - 2370

Published: March 6, 2025

Aeromonas hydrophila (A. hydrophila) is an opportunistic and foodborne pathogen widely spread in the environments, particularly aquatic environments. Diseases caused by A. freshwater aquaculture result huge economic losses every year. The increasing emergence of antibiotic resistance has limited application antibiotics aquaculture. Aerolysin (AerA), main virulence factor produced hydrophila, been identified as a promising target for developing drugs controlling infection. Here, we found that natural compound amentoflavone (AMF) with MIC 512 μg/mL against could dose-dependently reduce hemolysis AerA, ranging from 0.5 to 4 μg/mL. Molecular docking dynamics simulation results predicted AMF directly bind domain 3 AerA via Pro333 Trp375 residues. Then, binding sites were confirmed fluorescence quenching assays. heptamer formation demonstrated affect oligomers loss pore-forming activity. Cell viability assay showed after treatment significantly AerA-mediated cytotoxicity. Moreover, experimental therapeutics channel catfish infected then administered 20 mg/kg at intervals 12 h days increase survival rate 35% compared positive control 10-day observation. These findings provided novel approach anti-infective candidate infection

Language: Английский

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