Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 12, 2024
Abstract
While
neurosurgical
interventions
are
frequently
used
in
laboratory
mice,
refinement
efforts
to
optimize
analgesic
management
based
on
multimodal
approaches
appear
be
rather
limited.
Therefore,
we
compared
the
efficacy
and
tolerability
of
combinations
non-steroidal
anti-inflammatory
drug
carprofen,
a
sustained-release
formulation
opioid
buprenorphine,
local
anesthetic
bupivacaine
with
carprofen
monotherapy.
Female
male
C57BL/6J
mice
were
subjected
isoflurane
anesthesia
an
intracranial
electrode
implant
procedure.
Given
multidimensional
nature
postsurgical
pain
distress,
various
physiological,
behavioral,
biochemical
parameters
applied
for
their
assessment.
The
analysis
revealed
alterations
Neuro
scores,
home
cage
locomotion,
body
weight,
nest
building,
mouse
grimace
scales,
fecal
corticosterone
metabolites.
A
composite
measure
scheme
allowed
allocation
individual
severity
classes.
comparison
between
groups
failed
indicate
superiority
regimens
over
high-dose
NSAID
In
conclusion,
our
findings
confirmed
informative
value
assessment
distress
following
procedures
mice.
all
well
tolerated
control
data
suggest
that
total
load
should
carefully
considered
perioperative
management.
Future
studies
would
interest
assess
potential
synergies
lower
doses
carprofen.
Frontiers in Animal Science,
Journal Year:
2021,
Volume and Issue:
2
Published: Aug. 23, 2021
“Smart”
or
“precision”
farming
has
revolutionized
crop
agriculture
but
its
application
to
livestock
raised
ethical
concerns
because
of
possible
adverse
effects
on
animal
welfare.
With
rising
public
concern
for
welfare
across
the
world,
some
people
see
efficiency
gains
offered
by
new
technology
as
a
direct
threat
animals
themselves,
allowing
producers
get
“more
less”
in
interests
profit.
Others
major
advantages
through
life-long
health
monitoring,
delivery
individual
care
and
optimization
environmental
conditions.
The
answer
question
whether
smart
improves
damages
is
likely
depend
three
main
factors.
Firstly,
much
will
how
defined
extent
which
politicians,
scientists,
farmers
members
can
agree
what
means
so
come
common
view
judge
it
impacted
technology.
Defining
combination
good
themselves
want
provides
unifying
animal-centered
way
forward.
It
also
be
directly
adapted
computer
recognition
A
second
critical
factor
high
standards
are
made
priority
within
systems.
To
achieve
this,
necessary
both
develop
algorithms
that
recognize
satisfaction
build
into
control
decision-making
What
matter
most
end,
however,
third
factor,
actually
deliver
promised
improvements
when
applied
real
world.
An
evaluation
only
technologies
more
widely
deployed
commercial
farms
their
full
social,
environmental,
financial
implications
become
apparent.
animal,
Journal Year:
2022,
Volume and Issue:
16(9), P. 100614 - 100614
Published: Aug. 16, 2022
Virtual
fencing
(VF)
represents
a
way
to
simplify
traditional
pasture
management
with
its
high
labour
and
cost
requirements
for
make
better
use
of
the
'beneficial'
agronomic
ecological
effects
livestock
grazing.
In
this
study,
VF
technology
(®
Nofence,
AS,
Batnfjordsøra
Norway)
was
used
Fleckvieh
heifers
investigate
possible
welfare
impacts
on
animals
compared
conventionally
fenced
when
they
were
trained
respond
correctly
system.
The
Nofence®
collars
(attached
neck
heifers)
send
acoustic
signals
as
warning
approach
line,
which
set
up
by
GPS
coordinates
within
Nofence®-App,
followed
an
electric
pulse
do
not
stop
or
return.
had
no
experience
prior
study.
Two
treatments
(VF
versus
physical
(PF))
applied
six
groups
four
each
(three
per
treatment)
over
three
12-day
time
replicates.
One
line
separated
group
into
accessible
non-accessible
area.
control
PF
line.
Both
equipped
(deactivated
group).
trial
took
place
two
adjacent
paddocks
1
000
m2
following
schedule
divided
sections:
visual
support
barrier
(first
2
days),
only
virtual
border
without
support,
moving
(on
day
8).
Each
replicate
next
successively
different
new
heifers,
grazed
5
h
daily.
During
whole
experiment,
behaviour
continuously
observed;
a.m.,
p.m.
Exclusion
effective
in
our
trial.
None
crossed
boundary,
i.e.
spent
exclusion
zone
zero.
received
2.70
±
2.63
0.30
0.36
pulses
(mean
SD)
heifer
hour
during
all
Main
cattle
affected
Live
weight
gain,
herbage
consumption
faecal
cortisol
metabolites
also
revealed
significant
differences.
duration
until
restarted
grazing
after
from
collar
significantly
shorter
than
fence.
We
can
summarise
that
well-being
negatively
PF.
Animals,
Journal Year:
2023,
Volume and Issue:
13(12), P. 1966 - 1966
Published: June 12, 2023
Zoological
institutions,
such
as
zoos
and
aquariums,
have
made
animal
welfare
a
top
priority,
it
is
not
only
moral
obligation
but
also
crucial
for
fulfilling
their
roles
in
education
conservation.
There
need
science-based
tools
to
assess
monitor
these
settings.
However,
assessing
the
of
zoo
animals
challenging
due
diversity
species
lack
knowledge
on
specific
needs.
This
review
aims
discuss
advantages
disadvantages
existing
methodologies
through:
(1)
A
critical
analysis
main
approaches
assessment;
(2)
description
most
relevant
animal-based
indicators
with
particular
focus
behavioural
physiological
indicators;
(3)
An
identification
areas
that
require
further
research.
Metabolites,
Journal Year:
2023,
Volume and Issue:
13(1), P. 106 - 106
Published: Jan. 9, 2023
The
main
glucocorticoids
involved
in
the
stress
response
are
cortisol
and
cortisone
most
mammals
corticosterone
birds
rodents.
Therefore,
these
analytes
currently
biomarkers
more
frequently
used
to
evaluate
physiological
a
stressful
situation.
In
addition,
“total
glucocorticoids”,
which
refers
quantification
of
various
by
immunoassays
showing
cross-reactivity
with
different
types
or
related
metabolites,
can
be
measured.
this
review,
we
describe
characteristics
assess
stress,
as
well
techniques
samples
for
their
quantification.
analyse
studies
where
at
least
two
were
measured
combination.
Overall,
review
points
out
behaviours
glucocorticoids,
depending
on
animal
species
stimuli,
shows
potential
advantages
that
measurement
glucocorticoid
have
evaluating
welfare.
Frontiers in Ecology and Evolution,
Journal Year:
2023,
Volume and Issue:
11
Published: March 31, 2023
The
eastern
gray
squirrel
(
Sciurus
carolinensis
)
has
been
labeled
as
one
of
the
100
worst
invasive
alien
species
by
IUCN.
In
Europe,
introduced
to
Britain,
Ireland
and
Italy,
its
subsequent
spread
resulted
in
wide-scale
extinction
native
Eurasian
red
squirrels
vulgaris
from
areas
colonized
squirrel.
This
replacement
a
an
competitor
is
best
documented
cases
devastating
effects
biological
invasions
on
fauna.
To
understand
how
this
occurs,
we
present
systematic
review
literature
competition
interactions
between
squirrels.
We
describe
patterns
distribution
those
parts
Europe
where
occur
summarize
evidence
different
processes
mechanisms
determining
outcome
including
influence
predators
pathogens.
Some
drivers
behind
demise
have
intensively
studied
past
30
years,
but
recent
field
studies
mathematical
models
revealed
that
underlying
red-gray
paradigm
are
more
complex
than
previously
thought
affected
landscape-level
processes.
Therefore,
consider
habitat
type
multi-species
interactions,
host-parasite
predator-prey
relationships,
determine
interaction
two
better
address
control
efforts.
Scientific Reports,
Journal Year:
2020,
Volume and Issue:
10(1)
Published: July 15, 2020
Abstract
Poor
reproducibility
is
considered
a
serious
problem
in
laboratory
animal
research,
with
important
scientific,
economic,
and
ethical
implications.
One
possible
source
of
conflicting
findings
research
are
environmental
differences
between
facilities
combined
rigorous
standardization
within
studies.
Due
to
phenotypic
plasticity,
study-specific
conditions
during
development
can
induce
the
animals’
responsiveness
experimental
treatments,
thereby
contributing
poor
results.
Here,
we
studied
how
variation
weaning
age
(14–30
days)
housing
(single
versus
group
housing)
affects
phenotype
SWISS
mice
as
measured
by
range
behavioral
physiological
outcome
variables.
Weaning
age,
conditions,
their
interaction
had
little
effect
on
stereotypies,
well
body
weight,
glucocorticoid
metabolite
concentrations,
behavior
elevated
plus-maze
open
field
test.
These
results
surprising
partly
conflict
previously
published
findings,
especially
respect
effects
early
weaning.
Our
thus
question
external
validity
previous
call
for
further
identify
sources
replicate
studies
study
designs
that
produce
robust
reproducible
Metabolites,
Journal Year:
2020,
Volume and Issue:
10(9), P. 371 - 371
Published: Sept. 15, 2020
The
demand
for
vitamin
D
analysis
in
veterinary
species
is
increasing
with
the
growing
knowledge
of
extra-skeletal
role
plays
health
and
disease.
circulating
25-hydroxyvitamin-D
(25(OH)D)
metabolite
used
to
assess
status,
benefits
analysing
other
metabolites
complex
pathway
are
being
discovered
humans.
Profiling
by
liquid
chromatography
tandem
mass
spectrometry
(LC-MS/MS)
facilitates
simultaneous
multiple
a
single
sample
over
wide
dynamic
ranges,
this
method
now
considered
gold-standard
quantifying
metabolites.
However,
very
few
studies
report
using
LC-MS/MS
species.
Given
complexity
similarities
roles
disease
between
humans
companion
animals,
there
clear
need
establish
comprehensive,
reliable
that
comparable
human
clinical
practice.
In
review,
we
highlight
differences
metabolism
measuring
beyond
25(OH)D.
Finally,
discuss
analytical
challenges
profiling
focus
on
methods.
Integrative and Comparative Biology,
Journal Year:
2020,
Volume and Issue:
60(1), P. 79 - 88
Published: Feb. 20, 2020
The
hypothalamic-pituitary-adrenal
(HPA)
axis
regulates
the
secretion
of
glucocorticoids,
hormones
with
diverse
roles
ranging
from
regulating
daily
metabolic
demand
to
coping
sudden
perturbations.
As
a
result,
glucocorticoids
are
thought
help
vertebrates
track
their
changing
environments
and
coordinate
plasticity
in
phenotypes.
While
this
endocrine
system
is
highly
plastic-where
one
individual
can
produce
multiple
phenotypes
across
varying
environmental
conditions-little
understood
about
degree
which
individuals,
populations,
or
species
differ
circulating
glucocorticoid
plasticity.
Empirical
research
quantifying
variation
has
increased
recent
years,
though
complex
HPA-axis
make
it
challenging
generalize
extent
exists.
I
provide
an
overview
current
findings
on
plasticity,
outline
types
researchers
should
consider
future
work
advance
our
understanding
causes
consequences