Bioassay and NMR-HSQC-Guided Isolation and Identification of Phthalide Dimers with Anti-Inflammatory Activity from the Rhizomes of Angelica sinensis

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Under the guidance of a bioactivity-guided approach and HSQC characteristic signals, nine pairs phthalic dimers, angesilides A-I (1-9), with complex polycyclic skeletons featuring bridged, fused, spiro ring systems were discovered from rhizomes Angelica sinensis. Their structures absolute configurations established by comprehensive spectroscopic methods, X-ray diffraction analysis, chiral separation. Plausible biosynthetic pathway for 1-9 was proposed. Moreover, anti-inflammatory activities all isolates evaluated in lipopolysaccharide (LPS) stimulated mouse leukemia cells monocyte macrophages (RAW = 264.7). Among them, compound 9 showed remarkable inhibitory activity IC50 values 425 nM could significantly decrease IL-1β IL-6 transcription levels. Interestingly, (+)-9 has superior NO compared to that (-)-9, which verified further molecular docking analysis. The findings provide new insights into A. sinensis as functional food or development drug candidates.

Language: Английский

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Bioassay-guided and DeepSAT + SMART-driven identification of hepatoprotective phenolics from the fruits of Phyllanthus emblica

Fitoterapia, Journal Year: 2025, Volume and Issue: 182, P. 106475 - 106475

Published: March 11, 2025

Language: Английский

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Structurally Diverse Quinoline Alkaloids With Antileukaemia Activities From Taraxacum mongolicum

Journal of Food Biochemistry, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Taraxacum mongolicum , a medicinal and food homologous traditional Chinese medicine, has important research value in clinical practice. Eight new quinoline alkaloids ( 1 – 8 ), including 1N‐geranyl 3 3,4‐phenylquinoline 4 6 ) 10‐carbonyl‐11‐pyrrolyl 7 8) twelve known alkaloid derivatives 9 20 were isolated from T. for the first time. These compounds elucidated according to analysing NMR, MS, UV IR spectra compared with references. Among them, 14 16 showed certain antileukaemia activities, which exhibited cell growth inhibitory activities against HL‐60 K562 cells than adriamycin, could be potential agents. The plausible biogenetic pathway preliminary structure–activity relationship of selected scientifically summarised discussed this work.

Language: Английский

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Larvicidal potential of Trachyspermum ammi essential oil and Delphinium speciosum extract against malaria, dengue, and filariasis mosquito vectors

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 5, 2024

Language: Английский

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Structurally Diverse Quinoline Alkaloids with Anti-Leukaemia Activities from Taraxacum Mongolicum

Published: Jan. 1, 2024

Language: Английский

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Hypecotumines A-D, new isoquinoline alkaloids with potential PCSK9 inhibition activity from Hypecoum erectum L.

Natural Products and Bioprospecting, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 15, 2024

Four new isoquinoline alkaloids, hypecotumines A-D (1-4), were isolated and identified from the whole herbs of Hypecoum erectum L. Their structures determined by a combination HRESIMS, NMR, X-ray diffraction analysis methods. Compounds 1-4 characterized terminal double bond at C-9 their plausible biosynthetic pathway was hypothesized. Since PCSK9 plays key role in development cardiovascular disease (CVD), exploration PCSK inhibitors natural products are beneficial for drug discovery CVD treatment. SPR Western blot assays showed compound 4 had inhibition activity with K

Language: Английский

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