Integrated Analysis of lncRNAs and mRNAs Reveals Complex Gene Network Mediated by lncRNAs and Regulatory Function of MuLRR-RLK-AS in Response to Phytoplasma Infection in Mulberry

Biomolecules, Journal Year: 2024, Volume and Issue: 14(3), P. 308 - 308

Published: March 5, 2024

Phytoplasma disease is one of the most serious infectious diseases that affects growth and development mulberry. Long non-coding RNAs (lncRNAs) play an important role in plants’ defense systems; however, contribution lncRNAs response to phytoplasma infection mulberry still largely unknown. Herein, strand-specific RNA sequencing was performed profile mRNAs involved mulberry, a total 4169 genes were found be differentially expressed (DE) between healthy phytoplasma-infected leaves. Moreover, 1794 identified, which 742 DE infected Target prediction showed there 68 44 may function as cis trans-regulators, targeting 54 genes, respectively. These target are associated with biological processes such metabolism, signaling, development, transcriptional regulation, etc. In addition, it expression antisense lncRNA (MuLRR-RLK-AS) leucine-rich repeat receptor-like protein kinase gene (MuLRR-RLK) decreased Interestingly, overexpression MuLRR-RLK-AS can inhibit MuLRR-RLK. levels PTI-related MAPK transgenic MuLRR-RLK Arabidopsis plants significantly higher than those wild-type when inoculated pathogens, conferred strong resistance. Our results demonstrate MuLRR-RLK-AS, trans-regulatory factor, negative regulatory factor for The information provided particularly useful understanding functions mechanisms

Language: Английский

---

Vvbzip22 Regulates Quercetin Synthesis to Enhances Cold Resistance in Grape

Published: Jan. 1, 2024

Grapes are one of the important fruit crops widely cultivated in world, with high nutritional and economic value. However, intensification global warming, extreme low-temperature has seriously affected development grape industry. Quercetin is a highly antioxidant active substance that can enhance tolerance plants to external environmental stress, but its function mechanism response stress grapes still unclear. Here, we found accumulate more quercetin under exogenous significantly improve cold resistance grapes. The key synthesis gene VvFLS1 up-regulated after treatment, overexpression increases content enhances grape. Yeast one-hybrid dual luciferase reporter systems demonstrate VvbZIP22 directly binds promoter, low-temperature-induced expression characteristics. Overexpression improves above results indicate plays an role stress. Under low temperatures, mediate through regulating VvFLS1, alleviate oxidative damage,

Language: Английский

---

Transcriptomic Profiles of Long Noncoding RNAs and Their Target Protein-Coding Genes Reveals Speciation Adaptation on the Qinghai-Xizang (Tibet) Plateau in Orinus

Biology, Journal Year: 2024, Volume and Issue: 13(5), P. 349 - 349

Published: May 16, 2024

Long noncoding RNAs (lncRNAs) are RNA molecules longer than 200 nt, which lack the ability to encode proteins and involved in multifarious growth, development, regulatory processes plants mammals. However, environmental-regulated expression profiles of lncRNAs

Language: Английский

---

Alternative splicing of lncRNA LAIR fine‐tunes the regulation of neighboring yield‐related gene LRK1 expression in rice

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 1751 - 1766

Published: June 29, 2024

SUMMARY The diversity in alternative splicing of long noncoding RNAs (lncRNAs) poses a challenge for functional annotation lncRNAs. Moreover, little is known on the effects alternatively spliced lncRNAs crop yield. In this study, we cloned nine isoforms resulting from lncRNA LAIR rice. are generated via 5′/3′ splice sites and different combinations specific introns. All activate expression neighboring LRK1 gene enhance yield‐related rice traits. addition, there slight differences binding ability to epigenetic modification‐related proteins OsMOF OsWDR5, which affect enrichment H4K16ac H3K4me3 at locus, consequently fine‐tune regulation These may be caused by polymorphic changes RNA secondary structure splicing. It was also observed that composition sensitive abiotic stress. findings suggest leads formation transcript population precisely regulates expression, relevant phenotypic polymorphism‐based breeding under changing environmental conditions.

Language: Английский

---

Interaction Between ncRNAs and Their Response Network in Plants Under Multi-stress Environment

Published: Jan. 1, 2024

Language: Английский

---