On techno-fixes in the era of crises: genomic interventions in food crops DOI

Emmanuel González-Ortega,

Alma Piñeyro‐Nelson, Claudio Martínez Debat

et al.

Agroecology and Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 49(4), P. 563 - 586

Published: Nov. 27, 2024

Agri-biotech promotes genetically modified organisms (GMO) as solutions to achieve food security by increasing yields, reducing pesticide use through introduced resistance herbicides and strategy for climate change mitigation adaptation. After 30 years of biotechnological crops, these proposals have failed on their pledges, while the risk assessment, non-considered outcomes environment, biodiversity health been overlooked. New Plant-Breeding Technologies (NPBTs), with capacity inserting, deleting, or modifying DNA RNA nucleotides in specific genetic regions emerged last 15 years. The most prominent technique used edit genomes is CRISPR-Cas. Its proponents argue that mutations via genome editing pose no environment and/or human health; thus, products should be exempted from any regulation, traceability labeling, a push would actually restrict freedom choose farmers consumers. Our work summarizes reported scientific literature gene tools crops. We discuss perspectives upgrading biosafety governance new GMO order promote autonomy farmers, away positivist techno-scientific fixes downplay systemic crises which industrial agriculture plays major role

Language: Английский

Semi‐automated workflow for high‐throughput Agrobacterium‐mediated plant transformation DOI Creative Commons
Davide Annese, Facundo Romani, Carolina Grandellis

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

High-throughput experiments in plants are hindered by long generation times and high costs. To address these challenges, we present an optimized pipeline for Agrobacterium tumefaciens transformation a simplified protocol to obtain stable transgenic lines of the model liverwort Marchantia polymorpha, paving way efficient high-throughput plant synthetic biology other applications. Our involves freeze-thaw method six-well plates that can be adapted robotic automation. Using Opentrons open-source platform, implemented semi-automated showing similar efficiency compared manual manipulation. Additionally, have streamlined process selection M. reducing cost, time, labor without compromising efficiency. The addition sucrose media significantly enhances production gemmae, accelerating isogenic plants. We believe protocols potential facilitate screenings diverse species represent significant step towards full automation pipelines. This approach allows testing ~100 constructs per month, using conventional tissue culture facilities. recently demonstrated successful implementation this screening hundreds fluorescent reporters gemmae.

Language: Английский

Citations

1
Genomic and physiological plasticity in natural variants of commercial soybean inoculants supports the non-GMO status of base-edited inoculants DOI

María Eugenia Cardillo,

Silvina Brambilla, Karen Liebrenz

et al.

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 161(1)

Published: April 1, 2025

Language: Английский

Citations

0
Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation DOI Creative Commons
Davide Annese, Facundo Romani, Carolina Grandellis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

ABSTRACT High-throughput experiments in plants are hindered by long generation times and high costs. To address these challenges, we present an optimized pipeline for Agrobacterium tumefaciens transformation simplified a protocol to obtain stable transgenic lines of the model liverwort Marchantia polymorpha , paving way efficient high-throughput plant synthetic biology other applications. Our involves freeze-thaw method 6-well plates that can be adapted robotic automation. Using Opentrons open-source platform, implemented semi-automated showing similar efficiency compared manual manipulation. Additionally, have streamlined process selection M. reducing cost, time, labour without compromising efficiency. The addition sucrose media significantly enhances production gemmae, accelerating isogenic plants. We believe protocols potential facilitate screenings diverse species represent significant step towards full automation pipelines. This approach allows testing ∼100 constructs per month, using conventional tissue culture facilities. recently demonstrated successful implementation this screening hundreds fluorescent reporters gemmae.

Language: Английский

Citations

0
On techno-fixes in the era of crises: genomic interventions in food crops DOI

Emmanuel González-Ortega,

Alma Piñeyro‐Nelson, Claudio Martínez Debat

et al.

Agroecology and Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 49(4), P. 563 - 586

Published: Nov. 27, 2024

Agri-biotech promotes genetically modified organisms (GMO) as solutions to achieve food security by increasing yields, reducing pesticide use through introduced resistance herbicides and strategy for climate change mitigation adaptation. After 30 years of biotechnological crops, these proposals have failed on their pledges, while the risk assessment, non-considered outcomes environment, biodiversity health been overlooked. New Plant-Breeding Technologies (NPBTs), with capacity inserting, deleting, or modifying DNA RNA nucleotides in specific genetic regions emerged last 15 years. The most prominent technique used edit genomes is CRISPR-Cas. Its proponents argue that mutations via genome editing pose no environment and/or human health; thus, products should be exempted from any regulation, traceability labeling, a push would actually restrict freedom choose farmers consumers. Our work summarizes reported scientific literature gene tools crops. We discuss perspectives upgrading biosafety governance new GMO order promote autonomy farmers, away positivist techno-scientific fixes downplay systemic crises which industrial agriculture plays major role

Language: Английский

Citations

0