Dissection of the genetic architecture of seed erucic acid content and consequences for breeding in Brassica napus L.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120727 - 120727

Published: Feb. 20, 2025

Language: Английский

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Identification of superior haplotype and haplotype combinations for UV-B stress-related genes in Rice (Oryza sativa L.)

Plant Biotechnology Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

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Comprehensive Analysis of Ghd7 Variations Using Pan-Genomics and Prime Editing in Rice

Genes, Journal Year: 2025, Volume and Issue: 16(4), P. 462 - 462

Published: April 17, 2025

The Ghd7 gene in rice plays a crucial role determining heading date, plant height, and grain yield. However, the variations their functional implications across different accessions are not fully understood. Based on release of large amount genome data recent years, we investigated through pan-genome analysis 372 diverse varieties figured out structural (SVs) locus. due to high cost pan-genomes, most genomes based next-generation sequencing (NGS) now. Therefore, developed method for identifying SVs using NGS Polymerase Chain Reaction (PCR) results identified 977 carrying such Ghd7. Furthermore, 46 single-nucleotide polymorphisms (SNPs) one insertion-deletion (InDel) coding region They classified into 49 haplotypes. Notably, splice-site mutation haplotype H6 causes aberrant mRNA splicing. Using prime editing (PE) technology, successfully restored Yixiang 1B (YX1B), delaying date by approximately 16 days. This modification synchronized between YX1B restorer line Yahui 2115 (YH2115R), enhancing hybrid seed production efficiency. In conclusion, our findings highlight potential integrating pan-genomics precision accelerate crop improvement enhance agronomic traits.

Language: Английский

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Accelerating wheat improvement through trait characterization: advances and perspectives

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(5)

Published: Sept. 1, 2024

Abstract Wheat ( Triticum spp.) is a primary dietary staple food for humanity. Many wheat genetic resources with variable genomes have record of domestication history and are widespread throughout the world. To develop elite varieties, agronomical stress‐responsive trait characterization foremost evaluating existing germplasm to promote breeding. However, genomic complexity one impediments mining characterization. Multiple reference cutting‐edge technologies like haplotype mapping, selection, precise gene editing tools, high‐throughput phenotyping platforms, high‐efficiency transformation systems, speed‐breeding facilities transforming functional genomics research understand diversity polyploidy. This review focuses on achievements in genomics, available omics approaches, bioinformatic developed past decades. Advances system biology approaches highlighted circumvent bottlenecks phenotypic as well transfer. In addition, we propose conducting studies developing sustainable breeding strategies wheat. These developments understanding traits speed up creation high‐yielding, stress‐resistant, nutritionally enhanced which will help addressing global security agricultural sustainability era climate change.

Language: Английский

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Genetic variation and assessment of seven salt tolerance genes in an Indica/Xian rice population

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Soil salinity is a major abiotic stressor that influences rice during the entire growth period. Breeding and planting salt-tolerant (ST) an efficient strategy for sustainable agriculture. However, only few elite natural variations conferring salt tolerance have been identified, distribution genetic effects of those ST genes remain poorly understood. Here, we investigated seven with nine ST-associated single nucleotide polymorphisms (SNPs) in sequenced Indica/Xian population comprising 550 accessions. On basis genotyping SNPs, all accessions were categorized into 21 haplotypes, each which contained at least four genes. Among SKC1^184H OsHKT1;1^94K relatively rarely distributed this population. Comparative analysis ST grouped haplotypes different target genotypes was performed, validated on SKC1, OsHKT2;3, OsHKT1;1, RST1 OsWRKY53 multiple Interestingly, found plays synergistic roles SKC1^140A SKC1^184H may originate from carrying SKC1^140A. RST1^530A previously reported to be associated sensitivity, but it should corrected concurrently RST1^611G study. Moreover, participate after extended treatment, expression OsWRKY53^173G positively correlated SKC1 conditionally participated dependent either or SKC1^184H. Overall, our results provide further insight mechanism marker-assisted selection improvement rice.

Language: Английский

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Genome- and transcriptome-wide association studies reveal the genetic basis of seed palmitic acid content in Brassica napus

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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