Amino acid metabolism pathways as key regulators of nitrogen distribution in tobacco: insights from transcriptome and WGCNA analyses

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 27, 2025

Nitrogen (N) is crucial for plant growth and distributed across various N morphologies within organs. However, the mechanisms controlling distribution of these are not fully understood. This study investigated key amino acid (AA) biosynthesis pathways regulating their impact on physiology growth. We examined in leaves, stems, roots two tobacco cultivars (Hongda K326) under different treatments at 75, 100 days after transplanting (DAT). Transcriptome analysis was performed 75 DAT to explore AA metabolism pathways. Weighted gene co-expression network (WGCNA) identified distribution, Mantel test assessed treatments, stages, distribution. Statistically significant differences were observed environmental conditions, cultivars, organs (p < 0.05). WGCNA phenylalanine (ko00360), alanine, aspartate, glutamate (ko00250), glycine, serine, threonine (ko00260) Nin-SDS (sodium dodecyl sulfate insoluble N), NW (water soluble NS respectively. Increased application promoted accumulation, while earlier stages cultivar Hongda favored inhibited high conditions. Gene expression correlated with biomass, accumulation. elucidates tobacco, emphasizing role These findings essential improving utilization optimizing management practices, ultimately enhancing crop productivity supporting sustainable agricultural practices.

Language: Английский

Impact of nitrogen fertilizer types and concentrations on soil seed bank germination and seedling growth in semi-fixed dunes of the Horqin Sandy Land

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 383, P. 125464 - 125464

Published: April 22, 2025

Language: Английский