Unveiling HSP40/60/70/90/100 Gene Families and Abiotic Stress Responses in Jerusalem Artichoke DOI

Wencai Ren,

Baishui Ding,

Wenhan Dong

et al.

Published: Jan. 1, 2023

Heat shock proteins (HSPs) are essential for plant growth, development, and stress adaptation. However, their roles in Jerusalem artichoke largely unexplored. Using bioinformatics, we classified 143 HSP genes into distinct families: HSP40 (82 genes), HSP60 (22 HSP70 (29 HSP90 (6 HSP100 (4 genes). Our analysis covered traits, evolution, structures. RNA-seq data, uncovered unique expression patterns of these across growth stages tissues. Notably, HSP40, HSP60, HSP70, HSP90, families each had specific roles. We also studied how gene responded to various stresses, from extreme temperatures drought salinity, revealing intricate dynamics. Remarkably, showed remarkable flexibility, while specifically types. Moreover, our unveiled significant correlations between pairs under stress, implying cooperative interactions. qRT-PCR validation underscored the significance particular such as HtHSP60-7, HtHSP90-5, HtHSP100-2, HtHSP100-3 responding stress. In summary, study advances understanding collectively manage stresses artichoke. This provides insights functions broader responses.

Language: Английский

Expression analysis of the apple HSP70 gene family in abiotic stress and phytohormones and expression validation of candidate MdHSP70 genes DOI Creative Commons
Min Liu,

Zhiyuan Bian,

Miao Shao

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 14, 2024

Heat shock protein 70 (HSP70) is one kind of molecular chaperones which are widely found in organisms, and its members highly conserved among each other, with important roles plant growth development. In this study, 56 HSP70 genes were identified from the apple genome database. Analysis gene duplication events showed that tandem segmental play an role promoting amplification MdHSP70 family. Collinearity analysis family more closely related to Arabidopsis, tomato soybean. The promoter region contains a large number cis-acting elements response hormones stress. Tissue-specific expression some associated various stages process. Codon preference small differences between codon bases 1 3 genome, base composition had effect on usage preference. multiple patterns suggested development hormonal abiotic stresses. yeast two-hybrid (Y2H) demonstrated MdHSP70-53 interacts MdDVH24_032563. qRT-PCR most members' stresses (MdHSP70-6, MdHSP70-26 MdHSP70-45) appeared be expressed. To further elucidate function (6, 26, 45), we introduced them into tobacco confirm subcellular locations noted these located cytoplasm cell membrane. This study serves as theoretical basis for studies helps investigate functional characterization gene.

Language: Английский

Citations

3
RsPDR8, a member of ABCG subfamily, plays a positive role in regulating cadmium efflux and tolerance in radish (Raphanus sativus L.) DOI
Xinyu Zhang,

Yingfei Ma,

Deqiang Lai

et al.

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 205, P. 108149 - 108149

Published: Oct. 29, 2023

Language: Английский

Citations

9
RsVQ4-RsWRKY26 module positively regulates thermotolerance by activating RsHSP70-20 transcription in radish (Raphanus sativus L.) DOI
Qing He, Min He, Xiaoli Zhang

et al.

Environmental and Experimental Botany, Journal Year: 2023, Volume and Issue: 214, P. 105467 - 105467

Published: Aug. 8, 2023

Language: Английский

Citations

8
Comprehensive Genomic Analysis and Expression Profile of Hsp70 Gene Family Related to Abiotic and Biotic Stress in Cucumber DOI Creative Commons

Zixian Zhou,

Lingdi Xiao,

Jindong Zhao

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(9), P. 1057 - 1057

Published: Sept. 21, 2023

Heat shock protein 70 (Hsp70) is a class of HSPs involved in plant growth and development, stress response regulation. The Hsp70 proteins exist widely the world, but detail information about Hsp70s still unclear cucumber. Based on available cucumber genome, total 12 genes (CsHsp70-1 to CsHsp70-12) were identified this study, they distributed among five out seven chromosomes. CsHsp70s divided into four groups based phylogenetic analysis by using sequences from other plants, their conserved motifs relatively conserved. Gene duplication showed that segmental main driving force expansion CsHsp70 genes. Promoter contained many cis-acting elements hormone responses. Expression RNA-seq qRT-PCR indicated expression most was associated with multiple biotic abiotic stresses This study introduces characteristics regulation levels various stresses, which provided basis for functional exploration utilization future.

Language: Английский

Citations

8
Heat shock responsive genes in Brassicaceae: genome-wide identification, phylogeny, and evolutionary associations within and between genera DOI
Aldrin Y. Cantila, Sheng Chen, Kadambot H. M. Siddique

et al.

Genome, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Heat stress affects the growth and development of Brassicaceae crops. Plant breeders aim to mitigate effects heat by selecting for tolerance, but genes responsible in remain largely unknown. During stress, shock proteins (HSPs) function as molecular chaperones aid protein folding, transcription factors (HSFs) serve transcriptional regulators HSP expression. We identified 5002 related genes, including HSPs HSFs, across 32 genomes Brassicaceae. Among these, 3347 were duplicated, with segmented duplication primarily contributing their expansion. 466 physical gene clusters, 240 homogenous clusters 226 heterogeneous shedding light on organization genes. Notably, 37 co-located published thermotolerance quantitative trait loci, which supports functional role conferring tolerance. This study provides a comprehensive resource identification elucidates clustering patterns establishes genomic foundation future tolerance research. hypothesise that genetic variants HSF certain species have potential improving

Language: Английский

Citations

2
Unveiling HSP40/60/70/90/100 gene families and abiotic stress response in Jerusalem artichoke DOI

Wencai Ren,

Baishui Ding,

Wenhan Dong

et al.

Gene, Journal Year: 2023, Volume and Issue: 893, P. 147912 - 147912

Published: Oct. 18, 2023

Language: Английский

Citations

5
Genome-wide identification of the Hsp70 gene family in Penaeus chinensis and their response to environmental stress DOI Creative Commons
Xinran Li, Miao Zhu

Animal Biotechnology, Journal Year: 2024, Volume and Issue: 35(1)

Published: April 23, 2024

The heat shock protein 70 (HSP70) gene family plays a crucial role in the response of organisms to environmental stress. However, it has not been systematically characterized shrimp. In this study, we identified 25

Language: Английский

Citations

0
Combined genomic, transcriptomic, and metabolomic analyses provide insights into the fruit development of bottle gourd (Lagenaria siceraria) DOI Creative Commons
Xuelian He, Yanyan Zheng,

Songguang Yang

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 12(3)

Published: Nov. 27, 2024

Bottle gourd (Lagenaria siceraria (Molina) Standl) is a widely distributed Cucurbitaceae species, but gaps and low-quality assemblies have limited its genomic study. To address this, we assembled nearly complete, high-quality genome of the bottle (Pugua) using PacBio HiFi sequencing Hi-C correction. The genome, being 298.67 Mb long with ContigN50 28.55 Mb, was identified to possess 11 chromosomes, centromeres, 18 telomeres, 24 439 predicted protein-coding genes; notably, gap-free telomere-to-telomere assembly accomplished for seven chromosomes. Based on Pugua transcriptomic metabolomic combined analyses revealed that amino acids lipids accumulate during expansion stage, while sugars terpenoids increase ripening. GA4 genes Aux/IAA family mediate fruit maturation, cell wall remodeling regulated by factors such as XTHs, EXPs, polyphenols, alkaloids, contributing environmental adaptation. GGAT2 positively correlated glutamate, source umami, SUS5 SPS4 expression aligned sucrose accumulation. This study provides valuable genetic resource research, enhancing understanding evolution supporting further studies development, quality, improvement.

Language: Английский

Citations

0
Systematic identification and analysis of the HSP70 genes reveals MdHSP70-38 enhanced salt tolerance in transgenic tobacco and apple DOI

Xuanxuan Han,

Chunhui Song,

Sen Fang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 289, P. 138943 - 138943

Published: Dec. 18, 2024

Language: Английский

Citations

0
Unveiling HSP40/60/70/90/100 Gene Families and Abiotic Stress Responses in Jerusalem Artichoke DOI

Wencai Ren,

Baishui Ding,

Wenhan Dong

et al.

Published: Jan. 1, 2023

Heat shock proteins (HSPs) are essential for plant growth, development, and stress adaptation. However, their roles in Jerusalem artichoke largely unexplored. Using bioinformatics, we classified 143 HSP genes into distinct families: HSP40 (82 genes), HSP60 (22 HSP70 (29 HSP90 (6 HSP100 (4 genes). Our analysis covered traits, evolution, structures. RNA-seq data, uncovered unique expression patterns of these across growth stages tissues. Notably, HSP40, HSP60, HSP70, HSP90, families each had specific roles. We also studied how gene responded to various stresses, from extreme temperatures drought salinity, revealing intricate dynamics. Remarkably, showed remarkable flexibility, while specifically types. Moreover, our unveiled significant correlations between pairs under stress, implying cooperative interactions. qRT-PCR validation underscored the significance particular such as HtHSP60-7, HtHSP90-5, HtHSP100-2, HtHSP100-3 responding stress. In summary, study advances understanding collectively manage stresses artichoke. This provides insights functions broader responses.

Language: Английский

Citations

0