Phytochrome A in etiolated wild-type and albino barley seedlings: a far-red pulse induces interconversion between the two physicochemically and functionally distinct phyA types – phyA′ into phyA″ DOI
V.A. Sineshchekov, Larissa A. Koppel

Functional Plant Biology, Journal Year: 2025, Volume and Issue: 52(5)

Published: April 28, 2025

The phytochrome (phy) photoreceptors, comprising a small family of species, regulate plant development, being most active under red (R) and far-red (FR) light. One the major phytochromes, phyA, is unique among others: it mediates distinct photoreactions – very low fluence responses (VLFR), high irradiance (HIR), (LFR) characteristic phyB. This functional diversity likely to be connected with its heterogeneity: there are two native pools, possibly differing by serine phosphorylation at N-terminus phyA′ mediating VLFR phyA″ responsible for HIR LFR. In this work, we investigated their nature in vivo spectrofluorimetry, turning chlorophyll-less albino barley mutant. It was characterized both higher total phyA content proportion etiolated coleoptile tips. lack protochlorophyllide (PChlide) allowed characterization pools primary leaves (of mutant) phyA′/phyA″ same as coleoptiles, whereas substantially lower. mutant coleoptiles revealed less lability light compared wild type, suggesting that mutation may affect phyA′s proteolytic system. A specific effect FR on observed relatively fast (tens minutes) conversion into part complex process adaptation.

Language: Английский

Phytochrome A in etiolated wild-type and albino barley seedlings: a far-red pulse induces interconversion between the two physicochemically and functionally distinct phyA types – phyA′ into phyA″ DOI
V.A. Sineshchekov, Larissa A. Koppel

Functional Plant Biology, Journal Year: 2025, Volume and Issue: 52(5)

Published: April 28, 2025

The phytochrome (phy) photoreceptors, comprising a small family of species, regulate plant development, being most active under red (R) and far-red (FR) light. One the major phytochromes, phyA, is unique among others: it mediates distinct photoreactions – very low fluence responses (VLFR), high irradiance (HIR), (LFR) characteristic phyB. This functional diversity likely to be connected with its heterogeneity: there are two native pools, possibly differing by serine phosphorylation at N-terminus phyA′ mediating VLFR phyA″ responsible for HIR LFR. In this work, we investigated their nature in vivo spectrofluorimetry, turning chlorophyll-less albino barley mutant. It was characterized both higher total phyA content proportion etiolated coleoptile tips. lack protochlorophyllide (PChlide) allowed characterization pools primary leaves (of mutant) phyA′/phyA″ same as coleoptiles, whereas substantially lower. mutant coleoptiles revealed less lability light compared wild type, suggesting that mutation may affect phyA′s proteolytic system. A specific effect FR on observed relatively fast (tens minutes) conversion into part complex process adaptation.

Language: Английский

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