Plants,
Journal Year:
2024,
Volume and Issue:
13(21), P. 3109 - 3109
Published: Nov. 4, 2024
Low-temperature
freezing
stress
constitutes
the
most
significant
meteorological
disaster
during
overwintering
period
in
Nanfeng
Tangerine
(NT)
production
area,
severely
impacting
normal
growth
and
development
of
plants.
Currently,
accuracy
warnings
forecasts
for
NT
orchards
remains
suboptimal,
primarily
due
to
absence
quantitative
indicators
low-temperature
stress.
Therefore,
this
study
employed
plants
as
experimental
subjects
conducted
controlled
treatment
experiments
under
varying
intensities
(0
°C,
−2
−5
−7
−9
°C)
durations
(1
h,
4
7
h).
Subsequently,
physiological
biochemical
parameters
were
measured,
including
photosynthetic
parameters,
chlorophyll
fluorescence
reactive
oxygen
species,
osmoregulatory
substances,
antioxidant
enzyme
activities
The
results
demonstrated
that
adversely
affected
system
plants,
disrupted
dynamic
equilibrium
system,
compromised
cellular
stability.
severity
damage
increased
with
decreasing
temperature
prolonged
exposure.
Chlorophyll
(a/b)
ratio
(Chl
(a/b)),
maximum
quantum
yield
photosystem
II
(Fv/Fm),
soluble
sugar,
malondialdehyde
(MDA)
identified
key
assessing
changes
Utilizing
these
four
a
comprehensive
score
(CS)
model
was
developed
quantitatively
evaluate
status
across
gradients
durations.
grade
index
established.
Specifically,
Level
1
CS
≤
−0.50,
2
−0.5
<
0,
3
0
0.5,
0.5
CS.
research
provide
valuable
data
agricultural
departments
carry
out
monitoring,
early
warning,
prevention
control.
Frontiers in Plant Science,
Journal Year:
2024,
Volume and Issue:
15
Published: April 16, 2024
Among
tropical
fruit
trees,
coconut
holds
significant
edible
and
economic
importance.
The
natural
growth
of
coconuts
faces
a
challenge
in
the
form
low
temperatures,
which
is
crucial
factor
among
adverse
environmental
stresses
impacting
their
geographical
distribution.
Hence,
it
essential
to
enhance
our
comprehension
molecular
mechanisms
through
cold
stress
influences
various
varieties.
We
employed
analyses
leaf
morphology
physiological
traits
examine
how
respond
temperatures
over
2-hour,
8-hour,
2-day,
7-day
intervals.
Additionally,
we
performed
transcriptome
metabolome
identify
shifts
two
varieties
displaying
distinct
sensitivities
stress.
As
length
extended,
there
was
prominent
escalation
within
soluble
protein
(SP),
proline
(Pro)
concentrations,
activity
peroxidase
(POD)
superoxide
dismutase
(SOD)
leaves.
Contrariwise,
glutathione
(GSH)
underwent
substantial
reduction
during
this
period.
widespread
analysis
disclosed
nexus
genes
metabolites
intricately
were
chiefly
involved
pathways
centered
around
amino
acid,
flavonoid,
carbohydrate
lipid
metabolism.
perceived
several
stress-responsive
metabolites,
such
as
flavonoids,
carbohydrates,
lipids,
acids,
unveiled
considerably,
lower
genotype
subtle
Furthermore,
uncovered
pivotal
acid
biosynthesis,
antioxidant
system
flavonoid
biosynthesis
pathway
that
presented
down-regulation
sensitive
This
study
broadly
enriches
contemporary
perception
machinery
contributes
altering
levels
tolerance
amid
genotypes.
It
also
unlocks
unique
prospects
for
exploration
areas
breeding
or
engineering,
aiming
identifying
tolerant
and/or
encompassing
multi-omics
layers
response
conditions.
Agronomy,
Journal Year:
2025,
Volume and Issue:
15(2), P. 308 - 308
Published: Jan. 26, 2025
Caucasian
clover
(Trifolium
ambiguum
M.Bieb.)
is
a
perennial
legume
known
for
its
exceptional
cold
tolerance,
commonly
used
in
agriculture
and
ecosystems
climates.
Given
the
impact
of
climate
change,
enhancing
adaptation
crucial
sustaining
agricultural
productivity.
This
study
employs
metabolomics,
transcriptomics,
Weighted
Gene
Co-expression
Network
Analysis
(WGCNA)
to
investigate
molecular
mechanisms
clover’s
response
low-temperature
stress.
Metabolomic
analysis
showed
that
stress
triggered
accumulation
fatty
acids,
amino
antioxidants,
which
are
critical
maintaining
membrane
stability
antioxidant
capacity,
thus
protecting
plant
from
oxidative
damage.
Transcriptomic
revealed
significant
upregulation
genes
involved
adaptation,
particularly
those
related
defense,
lipid
repair,
signal
transduction,
including
ABA
signaling
pathway
enzymes,
thereby
improving
tolerance.
WGCNA
identified
gene
modules
closely
linked
especially
acid
metabolism,
repair.
These
function
synergistically,
with
coordinated
expression
resistance.
also
investigated
isoflavonoid
biosynthesis
under
stress,
highlighting
role
capacity
Low-temperature
induced
key
enzyme
genes,
such
as
Isoflavone
Synthase
(IFS)
Isoflavone-7-O-Glucosyltransferase
(IF7GT),
promoting
metabolite
further
plant’s
adaptation.
Overall,
this
offers
novel
insights
into
tolerance
provides
valuable
theoretical
support
breeding
cold-resistant
crops
BMC Genomics,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: Jan. 13, 2025
Cold
stress
in
winter
is
one
of
the
most
severe
abiotic
stresses
on
plant
growth
and
flourishing,
selection
cold
tolerant
genotypes
an
important
strategy
to
ensure
safety
development.
Cyclocarya
paliurus,
a
diclinous
versatile
tree
species
originally
subtropical
regions,
has
been
introduced
cultivated
warm
temperate
zone
China
meet
increasing
market
demand
for
its
leaf
yield.
However,
information
regarding
tolerance
remains
limited.
Based
ploidy
identification
tested
materials,
imitation
experiment
was
conducted
investigate
variation
freezing
injury
index
expression
CpaWRKY
family
members
diploid
tetraploid
C.
paliurus
seedlings.
The
results
indicated
significant
difference
between
diploids
tetraploids
under
imitating
temperature
southern
zone,
with
showing
better
than
tetraploids.
A
total
88
genes
were
identified
from
genome,
RNA-Seq
showed
differences
WRKY
gene
stress.
Correlation
analysis
differentially
expressed
suggested
that
CpaWRKY14,
CpaWRKY26
CpaWRKY86
play
essential
roles
respond
In
contrast,
major
involved
response
CpaWRKY60,
CpaWRKY63
CpaWRKY81.
Moreover,
CpaWRKY14
considerably
higher
compared
this
study
not
only
enhance
our
comprehension
role
stress,
but
also
provide
foundation
genetic
improvement
paliurus.
