ncRNAs in plant development and stress responses

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108950 - 108950

Published: July 18, 2024

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The Role of E3 Ubiquitin Ligase Gene FBK in Ubiquitination Modification of Protein and Its Potential Function in Plant Growth, Development, Secondary Metabolism, and Stress Response

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 821 - 821

Published: Jan. 19, 2025

As a crucial post-translational modification (PTM), protein ubiquitination mediates the breakdown of particular proteins, which plays pivotal role in large number biological processes including plant growth, development, and stress response. The ubiquitin-proteasome system (UPS) consists ubiquitin (Ub), ubiquitinase, deubiquitinating enzyme (DUB), 26S proteasome more than 80% degradation for turnover plants. For ubiquitinases, ubiquitin-activating (E1), ubiquitin-conjugating (E2), ligase (E3), FBK (F-box Kelch repeat protein) is an essential component multi-subunit E3 SCF (Skp1-Cullin 1-F-box) involved specific recognition target proteins UPS. Many genes have been identified different species, regulates growth development through affecting endogenous phytohormones as well tolerance to various biotic abiotic stresses associated with changes secondary metabolites such phenylpropanoid, phenolic acid, flavonoid, lignin, wax, etc. review summarizes significance protein, UPS degradation, possible function metabolism, response, provides systematic comprehensive understanding mechanism potential FBKs species.

Language: Английский

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Hydrogen Sulfide Induces Overaccumulation of Antioxidant Flavonoids Through the Regulatory Effect of Micrornas and its Target Genes in Arabidopsis

Published: Jan. 1, 2025

Language: Английский

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Multidimensional regulation of transcription factors: decoding the comprehensive signals of plant secondary metabolism

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Plants synthesize an extensive array of secondary metabolites in response to diverse biotic and abiotic stresses. These function not only as defensive compounds but also constitute significant sources nutrition pharmaceuticals. However, the mechanisms governing synthesis these have long been a central focus research continue pose challenges. Transcription factors (TFs), serving key regulators metabolite plants, exhibit action that are still fully understood. This review summarizes latest advancements on how plant transcription mediate regulation biosynthesis through various signaling pathways, including light signaling, hormone MAPK ubiquitin-proteasome pathway, epigenetic regulation, microbial interactions, climate change. A deeper understanding regulating is expected provide new insights into metabolites.

Language: Английский

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Screening Isodon rubescens-Derived Vesicles Harvested at Different Periods as Carriers for the Treatment of Colitis-Associated Cancer

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Plant-derived extracellular vesicles are of great significance in practical applications due to their availability, low immunogenicity, and effective carrier performance. Isodon rubescens-derived (IRDVs) not only have the capability for drug-carrying targeted therapy but also exhibit certain anti-inflammatory antitumor effects as part traditional Chinese medicine. The harvest time is closely related quality its medicinal ingredients; however, whether there differences IRDVs harvested at different times has yet be explored. Herein, we analyzed morphology chemical composition collected points identified six-month-harvested most suitable delivery vector. To further enhance therapeutic potential, modified with deoxycholic acid facilitate oral chemotherapy drug doxorubicin (DOX). This novel nanotherapy, termed DOX-IRDVs@DA, was developed an treatment colitis-associated cancer (CAC). results demonstrated that DOX-IRDVs@DA effectively delivered DOX colon tumor 26 (CT26) cells, induced cell apoptosis by modulating apoptosis-related proteins, inhibited proliferation CT26 cells. In vivo studies a mouse model CAC revealed achieved superior effects, reducing number colonic nodules, restoring structural integrity colon, causing minimal systemic toxicity.

Language: Английский

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Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 697 - 697

Published: April 25, 2025

Long non-coding RNAs (lncRNAs) have been defined as transcripts exceeding 200 nucleotides, which play essential roles during transcriptional and post-transcriptional regulation in multiple biological processes. Understanding R. delavayi lncRNAs is of great significance it profoundly influenced by heat stress. In this research, a total 1145 differentially expressed (DE-lncRNAs) 9447 genes (DEGs) (log2|FC| > 1, p < 0.05) were characterized between heat-stress-treated groups control groups. Further analysis showed that 229 DE-lncRNAs (144 upregulated 85 downregulated) commonly distributed two comparisons (CK_vs._H3 CK_vs._H6). We further investigated the cis- trans-acting DE-lncRNAs, found 142 corresponded to 1565 cis-acting DEGs, 143 3417 DEGs. KEGG enrichment these target revealed DEGs DE-lncRNA primarily enriched five twelve pathways, respectively. Co-expression network common pathways 57 co-expression relationships 28 43 554 26 90 Six six their used candidate verify RNA-seq data using qRT-PCR. three (TrxG, PEPC, CCR) out selected for subsequent research. This study examined relationship screened can potentially provide an important theoretical basis experimental genetic improvement tolerance delavayi.

Language: Английский

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Regulatory mechanisms of nitrogen homeostasis in insect growth and development

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Nitrogen is an essential element for the synthesis of proteins, nucleic acids, and various other critical biological molecules in insects. The maintenance nitrogen homeostasis insects achieved through a balance dietary intake, metabolic conversion, excretion. Insects primarily acquire from their diet, which subsequently metabolized into amino vital biomolecules following digestion absorption. Excess excreted forms such as uric acid, allantoin, allantoic urea, ammonia. Disruptions regulation can result ammonia toxicity abnormal production or excretion nitrogenous metabolites, including ultimately impairing insect development survival. This review examines mechanisms underlying insects, with focus on intricate regulatory roles carbohydrate metabolism, acid urea polyamine transport pathways, symbiotic interactions. By elucidating these processes, this aims to enhance our understanding nutritional metabolism developmental biology, while offering novel perspectives more effective pest management strategies.

Language: Английский

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Improving Nutritional Quality of Food Crops Using Nanomaterials and Nanostimulants

Published: Jan. 1, 2025

Language: Английский

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Integrative Analysis of Small RNA and Gene Expression Uncovers a Novel Long‐Noncoding MicroRNA Targeting a UDP‐Transferase in Camellia chekiangoleosa

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

ABSTRACT Biosynthesis of specific secondary metabolites in plants involves fine regulation gene expression. Camellia chekiangoleosa has important economic value: the seeds contain high‐quality unsaturated fatty acids and pericarp is rich tea saponins. As an posttranscriptional regulator, role microRNAs (miRNAs) controlling metabolism C. not fully studied. Here, we investigated miRNAs their targets metabolic regulatory network by comprehensively analyzing small RNAs, transcriptomes, degradomes from different tissues. We identified 168 known 74 novel genome revealed 15 tandem clusters containing 35 miRNAs. By establishing a miRNAs, target genes, transcription factors, unravelled multiplicity miRNA tissue‐specific expression, which may be tightly linked to synthesis metabolites. Furthermore, characterized long‐noncoding (cch‐miR3633) that targeted UDP‐transferase ( CchUGT94E5 ). showed that, ectopic expression caused outgrowth shoot branching changes cytokinin contents Arabidopsis , indicating potential regulating metabolism. This work provides valuable information for study

Language: Английский

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Integrative analysis of miRNA profile and degradome reveals post-transcription regulation involved in fragrance formation of Rosa rugosa

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135266 - 135266

Published: Sept. 6, 2024

Language: Английский

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