Enhanced Eicosapentaenoic Acid Production via Synthetic Biological Strategy in Nannochloropsis oceanica DOI Creative Commons

Congcong Miao,

Mingting Du, Hui Du

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(12), P. 570 - 570

Published: Dec. 19, 2024

The rational dietary ratio of docosahexaenoic acid (DHA) to eicosapentaenoic (EPA) can exert neurotrophic and cardiotrophic effects on the human body. marine microalga Nannochloropsis oceanica produces EPA yet no DHA, thus, it is considered an ideal EPA-only model pursue a DHA/EPA ratio. In this study, synthetic biological strategy was applied improve production in N. oceanica. Firstly, identify promoters terminators, fifteen genes from were isolated using transcriptomic approach. Compared α-tubulin, NO08G03500, NO03G03480 NO22G01450 exhibited 1.2~1.3-fold increases transcription levels. Secondly, EPA-synthesizing modules, putative desaturases (NoFADs) elongases (NoFAEs) overexpressed by NO08G03500 promoters/terminators wild type (WT), NoFAD1770 NoFAE0510 overexpression resulted 47.7% 40.6% yields, respectively. Thirdly, store triacylglycerol (TAG), NoDGAT2K promoter/terminator, along with NoFAD1770–NoFAE0510 stacking, forming transgenic line XS521. WT, TAG-EPA content increased 154.8% Finally, inhibit degradation, TAG lipase-encoding gene NoTGL1990 knocked out XS521, leading 49.2–65.3% increase content. Our work expands upon EPA-enhancing approaches through biology microalgae potentially crops.

Language: Английский

Enhancing lipid production in Nannochloropsis salina via RNAi-mediated downregulation of carbohydrate biosynthesis DOI Creative Commons
Hyun Gi Koh, Kyungmoon Park, See‐Hyoung Park

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 21, 2025

Microalgae are promising platforms for sustainable biofuel production owing to their high photosynthetic efficiency and carbon fixation capacity. Nannochloropsis salina is particularly valued its robust growth lipid accumulation. However, redirecting flux from carbohydrate biosynthesis remains a key challenge in microalgal metabolic engineering. In this study, RNA interference (RNAi) was employed downregulate uridine diphosphate-glucose pyrophosphorylase (UGPase), central enzyme chrysolaminarin biosynthesis. After confirming the presence of core RNAi machinery (Argonaute, Dicer, RDR) N. , an construct targeting UGPase introduced. Two transformants, NsRiUGPase 5 26, were selected through McrBC-PCR qRT-PCR screening based on reduced methylation-sensitive PCR band intensity transcript levels. These mutants exhibited significantly enhanced compared wild-type. On day 12, dry cell weight (DCW) reached 4.77 g/L 6.37 representing 32.4% 76.9% increases, respectively, WT (3.60 g/L). Despite similar contents per biomass, productivity markedly improved. 26 achieved 196.3 mg/L/day, 71.0% increase over (114.8 mg/L/day). Fatty acid methyl ester (FAME) analysis showed no significant difference composition among strains, indicating that knockdown did not affect quality. results demonstrate RNAi-mediated suppression successfully redirected away storage toward growth, thereby enhancing overall productivity. This study provides new insights into partitioning underscores as powerful tool optimization.

Language: Английский

Citations

0
Harnessing Microalgae: Pioneering Strategies for Cost-Effective EPA Synthesis DOI
Yiting Shen, Zixu Zhang, Xin Qi

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106687 - 106687

Published: April 1, 2025

Language: Английский

Citations

0
Editorial to special issue on Photosynthetic organisms for sustainable development DOI
Giorgio Perin, Alexandra Dubini, Francesco Milano

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 217, P. 109263 - 109263

Published: Nov. 1, 2024

Language: Английский

Citations

0
Enhanced Eicosapentaenoic Acid Production via Synthetic Biological Strategy in Nannochloropsis oceanica DOI Creative Commons

Congcong Miao,

Mingting Du, Hui Du

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(12), P. 570 - 570

Published: Dec. 19, 2024

The rational dietary ratio of docosahexaenoic acid (DHA) to eicosapentaenoic (EPA) can exert neurotrophic and cardiotrophic effects on the human body. marine microalga Nannochloropsis oceanica produces EPA yet no DHA, thus, it is considered an ideal EPA-only model pursue a DHA/EPA ratio. In this study, synthetic biological strategy was applied improve production in N. oceanica. Firstly, identify promoters terminators, fifteen genes from were isolated using transcriptomic approach. Compared α-tubulin, NO08G03500, NO03G03480 NO22G01450 exhibited 1.2~1.3-fold increases transcription levels. Secondly, EPA-synthesizing modules, putative desaturases (NoFADs) elongases (NoFAEs) overexpressed by NO08G03500 promoters/terminators wild type (WT), NoFAD1770 NoFAE0510 overexpression resulted 47.7% 40.6% yields, respectively. Thirdly, store triacylglycerol (TAG), NoDGAT2K promoter/terminator, along with NoFAD1770–NoFAE0510 stacking, forming transgenic line XS521. WT, TAG-EPA content increased 154.8% Finally, inhibit degradation, TAG lipase-encoding gene NoTGL1990 knocked out XS521, leading 49.2–65.3% increase content. Our work expands upon EPA-enhancing approaches through biology microalgae potentially crops.

Language: Английский

Citations

0