Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 1, 2025

ABSTRACT Necrotrophic pathogens cause serious threats to agricultural crops, and understanding the resistance genes their genetic networks is key breeding new plant cultivars with better traits. Although Alternaria alternata causes black spot in important leafy brassica vegetables, leads significant loss of yield food quality, little known about plant– A. interactions. In this study, we used a unique large collection single, double triple mutant lines defence metabolite regulators Arabidopsis explore how these transcription factors epistatic may influence infections. This identified nine novel 20 pairs interactions that modulate plants' responses infection. We further showed glucosinolate 4‐methoxy‐indol‐3‐ylmethyl only consistently responsive infection Col‐0 ecotype. With exploration on modulating accumulation glucosinolates under infection, an inverted triangle regulatory model was proposed for at metabolic level phenotypic level.

Language: Английский

---

Harnessing macroalgal cell walls to trigger immunity in Arabidopsis thaliana

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100783 - 100783

Published: Feb. 1, 2025

Language: Английский

---

Cell wall bricks of defence: the case study of oligogalacturonides

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 25, 2025

The plant cell wall (CW) is more than a structural barrier; it serves as the first line of defence against pathogens and environmental stresses. During pathogen attacks or physical damage, fragments CW, known CW-derived Damage-Associated Molecular Patterns (CW-DAMPs), are released. These molecular signals play critical role in activating plant’s immune responses. Among CW-DAMPs, oligogalacturonides (OGs), derived from breakdown pectin, some most well-studied. This review highlights recent advances understanding functional signalling roles OGs, beginning with their formation through enzymatic CW degradation during invasion mechanical injury. We discuss how OGs perception triggers intracellular pathways that enhance regulate interactions microbes. Given excessive OG levels can negatively impact growth development, we also examine regulatory mechanisms plants use to fine-tune responses, avoiding overactivation hyper- immunity. As natural modulators, (and generally CW-DAMPs), offer promising, sustainable alternative chemical pesticides by enhancing crop resilience without harming environment. By strengthening defences supporting eco-friendly agricultural practices, hold great potential for advancing resilient farming systems.

Language: Английский

---

Engineering and application of multiepitope recombinant proteins to enhance resistance to Botrytis cinerea in tomatoes: a new paradigm for creating plant immune activators

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 17, 2025

Plant elicitors have emerged as key agents in effectively invoking immune responses across various plant species, gaining attention for their role sustainable agricultural protection strategies. However, the economic utility of peptide such flg22, flgII-28, and systemin is limited when considering broader applications. This study introduces a novel recombinant protein, SlRP5, which integrates five active epitopes—flg22, csp22, SIPIP1, systemin—to activate significantly enhance resistance to Botrytis cinerea tomatoes ( Solanum lycopersicum ). SIRP5 induced reactive oxygen species (ROS), MAPK activation, callose deposition tomato leaves during vitro experiments. Transcriptomic analysis revealed that SlRP5 more activated immune-related pathways compared traditional peptides, upregulating critical genes involved calcium-binding proteins phenylpropanoid biosynthesis. In further vivo experiments, alleviated B. -induced membrane damage by reducing MDA REC levels, while simultaneously enhancing activities antioxidant enzymes SOD, CAT, POD, thereby mitigating excess ROS generated infection. Additionally, elicited significant immunological effects tobacco eggplant, characterized bursts deposition. It amplified tobacco’s TMV mitigated eggplant. These findings underscore substantial potential activator, integrating multiple immune-eliciting offering strategy cultivating new biopesticides can induce heighten disease crops.

Language: Английский

---

Potential of Bio-Sourced Oligogalacturonides in Crop Protection

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1392 - 1392

Published: March 20, 2025

During plant development or interactions with pathogens, modifications of the cell wall occur. Among enzymes involved, pectinases, particularly polygalacturonases (PGases), play a crucial role in controlled hydrolysis polysaccharides, leading to formation oligogalacturonides (OGs). These pectin-derived fragments act as key elicitors defense responses, stimulating innate immunity and enhancing resistance pathogens by modulating expression genes involved immune responses inducing production compounds. OGs are particular interest for protection natural alternative conventional phytosanitary products they can be obtained through chemical, thermal, enzymatic degradation biomass. In sustainable approach, agricultural by-products rich pectin, such citrus peels, apple pomace, sugar beet pulp, offer an eco-friendly cost-effective OG production. Thus, current review aims (i) update state art about different methods used produce OGs, (ii) explore potential bio-based biocontrol molecules, (iii) examine relevance new pectin sources

Language: Английский

---

Celery flavonoid-rich extract significantly reduces cucumber powdery mildew severity and enhances plant defense responses

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 27, 2025

This study assesses the antifungal efficacy of celery flavonoid-rich extract (CFRE) against cucumber powdery mildew, caused by Podosphaera fusca, in a controlled greenhouse setting. The application CFRE at concentration 4 mg mL− 1 resulted remarkable 97% reduction disease severity. High-performance liquid chromatographic (HPLC) analysis identified apigenin as predominant flavonoid CFRE. Furthermore, treatment induced robust defense response leaves, marked elevated levels flavonoids, phenolics, chlorophyll, and enzymes such β-1,3-glucanase, chitinase, peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase. also observed upregulation expression three investigated genes associated with ammonia-lyase. Notably, positive correlation was established between activity their gene expression, well antioxidant compounds. These findings underscore potential an environmentally benign alternative to chemical fungicides for managing P. fusca infections.

Language: Английский

---

Phenolic compounds-enriched extract recovered from two-phase olive pomace serves as plant immunostimulants and broad-spectrum antimicrobials against phytopathogens including Xylella fastidiosa

Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100655 - 100655

Published: Oct. 28, 2024

Language: Английский

---