Functional Characterization of Grapevine VviMYC4 in Regulating Drought Tolerance by Mediating Flavonol Biosynthesis DOI Creative Commons
Yun‐Hong Tan, Wenjuan Wang, Wen‐xia Tian

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1409 - 1409

Published: May 8, 2025

Drought ranks among the key abiotic stresses that limit growth and yield of grapevines (Vitis vinifera L.). Flavonols, a class antioxidants commonly found in grapevines, play crucial role combating drought stress. In this study, we characterized function regulatory mechanism grapevine VviMYC4 mediating flavonol biosynthesis response to encodes protein 468 amino acids with conserved bHLH-MYC_N bHLH domains. Phylogenetic analysis confirmed its homology VviMYC2 similarity function. The expression ‘Cabernet Sauvignon’ seedling leaves increased initially then decreased during prolonged homologous heterologous transformation grape suspension cells, Arabidopsis plants, tobacco leaves, demonstrated ability positively regulate accumulation by promoting flavonol-related genes, thereby enhancing tolerance transgenic plants. Furthermore, could bind specific E-box sites on promoters VviF3H VviFLS improve their activities. This study highlights as pivotal positive regulator proposes enhances antioxidant reactive oxygen species (ROS) scavenging abilities challenging environments improves stress resilience biosynthesis. Our findings offer candidate genes valuable insights for molecular breeding resistance.

Language: Английский

Functional Characterization of Grapevine VviMYC4 in Regulating Drought Tolerance by Mediating Flavonol Biosynthesis DOI Creative Commons
Yun‐Hong Tan, Wenjuan Wang, Wen‐xia Tian

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1409 - 1409

Published: May 8, 2025

Drought ranks among the key abiotic stresses that limit growth and yield of grapevines (Vitis vinifera L.). Flavonols, a class antioxidants commonly found in grapevines, play crucial role combating drought stress. In this study, we characterized function regulatory mechanism grapevine VviMYC4 mediating flavonol biosynthesis response to encodes protein 468 amino acids with conserved bHLH-MYC_N bHLH domains. Phylogenetic analysis confirmed its homology VviMYC2 similarity function. The expression ‘Cabernet Sauvignon’ seedling leaves increased initially then decreased during prolonged homologous heterologous transformation grape suspension cells, Arabidopsis plants, tobacco leaves, demonstrated ability positively regulate accumulation by promoting flavonol-related genes, thereby enhancing tolerance transgenic plants. Furthermore, could bind specific E-box sites on promoters VviF3H VviFLS improve their activities. This study highlights as pivotal positive regulator proposes enhances antioxidant reactive oxygen species (ROS) scavenging abilities challenging environments improves stress resilience biosynthesis. Our findings offer candidate genes valuable insights for molecular breeding resistance.

Language: Английский

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