Full-Length Transcriptome Analysis of Sesbania cannabina Stem Response to Waterlogging Stress DOI Creative Commons
Tingting He,

Guoli Sun,

Sunan He

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(5), P. 1197 - 1197

Published: May 15, 2025

Sesbania cannabina (Retz.) Pers., as a legume, has strong waterlogging tolerance, but the lack of genomic information limits exploration key genes and molecular mechanisms. In this study, single-molecule real-time technology was used to sequence stems RNA two varieties at five time points under stress through PacBio Iso-Seq platform. The full-length transcriptome contained 42 Gb raw data, 32,503 transcripts with an average length 1912.28 nt, N50 2059 nt GC content 42.69%. A total 32,143 coding sequences (CDSs), 1745 transcription factors (TFs), 282 long non-coding RNAs (LncRNAs), 7497 simple repeats (SSRs) 202 alternative splicing (AS) events were identified alignment software analysis. analysis showed that 10,075 enriched in 137 KEGG pathways, 519 included plant hormone signal transduction, which 103 123 were, respectively, involved ethylene auxin pathways. assembly annotation data provided reliable accurate for study mechanisms stem response stress.

Language: Английский

Full-Length Transcriptome Analysis of Sesbania cannabina Stem Response to Waterlogging Stress DOI Creative Commons
Tingting He,

Guoli Sun,

Sunan He

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(5), P. 1197 - 1197

Published: May 15, 2025

Sesbania cannabina (Retz.) Pers., as a legume, has strong waterlogging tolerance, but the lack of genomic information limits exploration key genes and molecular mechanisms. In this study, single-molecule real-time technology was used to sequence stems RNA two varieties at five time points under stress through PacBio Iso-Seq platform. The full-length transcriptome contained 42 Gb raw data, 32,503 transcripts with an average length 1912.28 nt, N50 2059 nt GC content 42.69%. A total 32,143 coding sequences (CDSs), 1745 transcription factors (TFs), 282 long non-coding RNAs (LncRNAs), 7497 simple repeats (SSRs) 202 alternative splicing (AS) events were identified alignment software analysis. analysis showed that 10,075 enriched in 137 KEGG pathways, 519 included plant hormone signal transduction, which 103 123 were, respectively, involved ethylene auxin pathways. assembly annotation data provided reliable accurate for study mechanisms stem response stress.

Language: Английский

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