Lipids in Equine Airway Inflammation: An Overview of Current Knowledge DOI Creative Commons
Jenni Mönki, Anna Mykkänen

Animals, Journal Year: 2024, Volume and Issue: 14(12), P. 1812 - 1812

Published: June 18, 2024

Mild–moderate and severe equine asthma (MEA SEA) are prevalent inflammatory airway conditions affecting horses of numerous breeds disciplines. Despite extensive research, detailed disease pathophysiology the differences between MEA SEA still not completely understood. Bronchoalveolar lavage fluid cytology, broadly used in clinical practice has limited means to represent status lower airways. Lipidomics is a field science that can be utilized investigating cellular mechanisms cell-to-cell interactions. Studies lipidomics have broad variety foci, which fatty acid lipid mediator profile analyses global been implemented veterinary medicine. As many crucial proinflammatory proresolving mediators lipids, lipidomic studies offer an interesting yet largely unexplored investigate reactions The aim this review article collect summarize findings recent on inflammation.

Language: Английский

Steatotic liver disease induced by TCPOBOP-activated hepatic constitutive androstane receptor: primary and secondary gene responses with links to disease progression DOI

Ravi Sonkar,

Hong Ma, David J. Waxman

et al.

Toxicological Sciences, Journal Year: 2024, Volume and Issue: 200(2), P. 324 - 345

Published: May 6, 2024

Constitutive androstane receptor (CAR, Nr1i3), a liver nuclear and xenobiotic sensor, induces drug, steroid, lipid metabolizing enzymes, stimulates hypertrophy hyperplasia, ultimately, hepatocellular carcinogenesis. The mechanisms linking early CAR responses to later disease development are poorly understood. Here we show that exposure of CD-1 mice TCPOBOP (1,4-bis[2-(3,5-dichloropyridyloxy)]benzene), halogenated xenochemical selective agonist ligand, pericentral steatosis marked by hepatic accumulation cholesterol neutral lipid, elevated circulating alanine aminotransferase, indicating hepatocyte damage. TCPOBOP-induced was weaker in the region but stronger periportal females compared with males. Early (1 day) transcriptional were enriched for CAR-bound primary response genes, lipogenesis metabolism oxidative stress protection pathways; late (2 weeks) included many binding-independent secondary enrichment macrophage activation, immune response, cytokine reactive oxygen species production. Late upstream regulators specific TCPOBOP-exposed male linked proinflammatory carcinoma progression. administered weekly using high corn oil vehicle induced carbohydrate-responsive transcription factor (MLXIPL)-regulated target dysregulated mitochondrial respiratory translation regulatory pathways, more advanced pathology. Overall, recapitulates histological gene expression changes characteristic emerging steatotic disease, including nonparenchymal cells indicative transition state. Upstream both genes include novel biomarkers foreign chemical-induced metabolic dysfunction-associated disease.

Language: Английский

Citations

2
Identification and functional characterization of a novel cystatin in amphioxus, ancient origin of vertebrate type-2 cystatin homologues DOI
Tianren Wang, Jingru Gao, Jinghan Xu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134429 - 134429

Published: Aug. 7, 2024

Language: Английский

Citations

2
Steatotic liver disease induced by TCPOBOP-activated hepatic constitutive androstane receptor: Primary and secondary gene responses with links to disease progression DOI Creative Commons

Ravi Sonkar,

Hong Ma, David J. Waxman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 20, 2024

Abstract Constitutive Androstane Receptor (CAR, Nr1i3 ), a liver nuclear receptor and xenobiotic sensor, induces drug, steroid lipid metabolizing enzymes, stimulates hypertrophy hyperplasia, ultimately, hepatocellular carcinogenesis. The mechanisms linking early CAR responses to subsequent disease development are poorly understood. Here we show that exposure of CD-1 mice TCPOBOP, halogenated xenochemical selective agonist ligand, pericentral steatosis marked by hepatic accumulation cholesterol neutral lipid, elevated circulating alanine aminotransferase levels, indicating hepatocyte damage. TCPOBOP-induced was weaker in the region but stronger periportal females compared males. Early (1-day) TCPOBOP transcriptional were enriched for CAR-bound primary response genes, metabolism oxidative stress protection pathways; late (2-wk) included many binding-independent secondary with enrichment immune response, macrophage activation, cytokine reactive oxygen species production. Late upstream regulators specific TCPOBOP-exposed male linked pro-inflammatory carcinoma progression. administered weekly using high corn oil vehicle activated carbohydrate-responsive transcription factor (MLXIPL)-regulated target dysregulated mitochondrial respiratory translation regulatory pathways, induced more advanced pathology. Thus, recapitulates histological gene expression changes characteristic emerging steatotic disease, including non-parenchymal cells indicative transition state. Upstream both include novel biomarkers foreign chemical-induced metabolic dysfunction-associated disease.

Language: Английский

Citations

1
Impaired fatty acid import or catabolism in macrophages restricts intracellular growth ofMycobacterium tuberculosis DOI Creative Commons
Nelson V. Simwela, Eleni Jaecklein, Christopher M. Sassetti

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Mycobacterium tuberculosis ( Mtb ) infection of macrophages reprograms cellular metabolism to promote lipid retention. While it is clearly known that intracellular utilize host derived lipids maintain infection, the role macrophage processing on bacteria’s ability access pool remains undefined. We utilized a CRISPR-Cas9 genetic approach assess impact sequential steps in fatty acid growth . Our analyzes demonstrate which cannot either import, store or catabolize acids restrict by both common and divergent anti-microbial mechanisms, including increased glycolysis, oxidative stress, production pro-inflammatory cytokines, enhanced autophagy nutrient limitation. also show impaired droplet biogenesis restrictive replication, but induction same fails rescue growth. work expands our understanding how homeostasis impacts macrophage.

Language: Английский

Citations

1
Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus DOI

Yi Zhao,

W. F. Mader, Bo Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134840 - 134840

Published: Aug. 31, 2024

Language: Английский

Citations

1
Lipids: Emerging Players of Microglial Biology DOI Creative Commons
Priya Prakash, Caitlin E. Randolph,

Katherine A. Walker

et al.

Glia, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Lipids are small molecule immunomodulators that play critical roles in maintaining cellular health and function. Microglia, the resident immune cells of central nervous system, regulate lipid metabolism both extracellular environment within intracellular compartments through various mechanisms. For instance, glycerophospholipids fatty acids interact with protein receptors on microglial surface, such as Triggering Receptor Expressed Myeloid Cells 2, influencing functions like phagocytosis migration. Moreover, cholesterol is essential not only for survival but, along other lipids acids, crucial formation, function, accumulation droplets, which modulate activity inflammatory diseases. Other lipids, including acylcarnitines ceramides, participate signaling pathways microglia. Despite complexity lipidome, a few studies have investigated effects specific classes biology. In this review, we focus major their modulating We also discuss novel analytical techniques characterizing lipidome highlight gaps current knowledge, suggesting new directions future research

Language: Английский

Citations

1
Dysregulation of macrophage lipid metabolism underlies intracellular bacterial neuroinvasion DOI Open Access

Zhou Sha,

Kun Yang, Shoupeng Fu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 28, 2024

Summary Acute infection of the central nervous system is one deadliest diseases, but mechanisms by which intracellular bacteria infiltrate brain remain poorly understood. Phagocytic cells are usually recognized as battlefield on war waged against bacteria; however, little known about how take advantage infected phagocytes to access brain. In this study, we find that a novel CD36 + Fabp4 Pparg macrophage subpopulation (CD36 macrophage) participates in penetration without disruption brain-blood barrier. Biomechanical analysis reveals abundant protrusions and adhesion molecules macrophages confer significant resistance mechanical stress blood flow, thereby providing more opportunities for these adhere vascular endothelial surface. Through metabolomics analysis, lipid metabolism dysregulated during bacterial neuroinvasion, β-hydroxybutyrate promotes formation survival macrophages. Importantly, ketogenesis exacerbates symptoms could be alleviated supplementing with physiological level glucose. Taken together, our findings uncover pathway hijack invade brain, suggesting might play role prevention or resolution neuroinvasion. Graphic Abstract

Language: Английский

Citations

0
Life cycle of macrophages in atherosclerotic inflammation progression and resolution: mediators and interventions (narrative review) DOI Creative Commons

Yuxiao Feng,

Qinlan Wang,

Bin He

et al.

Emergency and Critical Care Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

Abstract As one of the pathological causes coronary heart disease, atherosclerosis poses a major threat to human health. Macrophages play an important role in regulating atherosclerotic disease progression. Specifically, inflammation is initiated when low-density lipoproteins infiltrate subcutaneous area and are phagocytosed by macrophages, leading foam cell formation. The subsequent progression or resolution depends on delicate balance between proinflammatory anti-inflammatory mediators. In cases where factors dominate, macrophages tend activate pyroptosis necrosis pathways, resulting release intracellular damage-associated molecular patterns promoting necrotic core formation plaque Conversely, prevail, engage autophagy-mediated lipid metabolism while inhibiting through efferocytosis apoptotic cells. regulatory function can also be understood from perspective their life cycles. Lipid retention within arterial intima its uptake characteristic hallmarks atherosclerosis. pivotal effector cells this process, with distinctive performances decisively determine inflammation. complete cycle plaques encompasses chemotaxis, infiltration, polarization, for metabolic efflux, formation, overload, various forms programmed necrosis, including autophagy, pyroptosis, apoptosis, efferocytosis, facilitate removal limit behavior has rarely been comprehensively addressed previous review articles. This article provides extensive overview entire following response impact resolution. Considering that inflammatory associated atherosclerosis, targeting regulation holds promise therapeutic interventions against atherosclerosis-related cardiovascular diseases.

Language: Английский

Citations

0
Current approaches in CRISPR-Cas system for metabolic disorder DOI

Yajushii Arora,

Priya,

Manishankar Kumar

et al.

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Aug. 16, 2024

Language: Английский

Citations

0
Comparison of risk factors between lean and non-lean MASLD in individuals with type 2 diabetes: a multicenter study DOI

Weixia Sun,

Yingqi Lv, Li Wang

et al.

Endocrine Practice, Journal Year: 2024, Volume and Issue: 30(12), P. 1171 - 1179

Published: Sept. 25, 2024

Language: Английский

Citations

0