Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics DOI Creative Commons
Bin Xing,

Ruohong Wang,

Tianzhang Kou

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 30, 2025

Abstract Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding tissue‐specific biosynthesis and inter‐tissue crosstalk FFAs remains incomplete. In vivo stable‐isotope tracing metabolomics utilized to comprehensively measure FFA mice. Systematically assessing 13 across 15 tissues unveils dynamic spatial temporal accumulation redistribution throughout the body. Employing an analytical framework deconvolve mass isotopologue patterns, explored saturated, polyunsaturated, monounsaturated FFAs, quantify communications (16:0) (18:0) between liver other tissues. Then, decline acid peripheral but not brain aged mice observed, particularly evident palmitic acids. These findings illuminate complex interplay maintenance homeostasis.

Language: Английский

Metabolic Atlas of Early Human Cortex Identifies Regulators of Cell Fate Transitions DOI Creative Commons
Jessenya Mil, J Soto, Nedas Matulionis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Characterization of cell type emergence during human cortical development, which enables unique cognition, has focused primarily on anatomical and transcriptional characterizations. Metabolic processes in the brain that allow for rapid expansion, but contribute to vulnerability neurodevelopmental disorders, remain largely unexplored. We performed a variety metabolic assays primary tissue stem derived organoids observed dynamic changes core functions, including an unexpected increase glycolysis late neurogenesis. By depleting glucose levels organoids, we increased outer radial glia, astrocytes, inhibitory neurons. found pentose phosphate pathway (PPP) was impacted these experiments leveraged pharmacological genetic manipulations recapitulate glia fate changes. These data identify new role PPP modulating specification generate resource future exploration additional pathways development.

Language: Английский

Citations

0
Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouse DOI
Drew R. Seeger,

Peddanna Kotha,

Svetlana A. Golovko

et al.

Prostaglandins Leukotrienes and Essential Fatty Acids, Journal Year: 2025, Volume and Issue: unknown, P. 102679 - 102679

Published: March 1, 2025

Language: Английский

Citations

0
Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics DOI Creative Commons
Bin Xing,

Ruohong Wang,

Tianzhang Kou

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 30, 2025

Abstract Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding tissue‐specific biosynthesis and inter‐tissue crosstalk FFAs remains incomplete. In vivo stable‐isotope tracing metabolomics utilized to comprehensively measure FFA mice. Systematically assessing 13 across 15 tissues unveils dynamic spatial temporal accumulation redistribution throughout the body. Employing an analytical framework deconvolve mass isotopologue patterns, explored saturated, polyunsaturated, monounsaturated FFAs, quantify communications (16:0) (18:0) between liver other tissues. Then, decline acid peripheral but not brain aged mice observed, particularly evident palmitic acids. These findings illuminate complex interplay maintenance homeostasis.

Language: Английский

Citations

0