Pseudomonas
tolaasii
(P.
tolaasii)
is
considered
to
be
the
main
pathogen
of
brown
spot
disease
in
mushrooms.
Therefore,
P.
was
isolated
from
browning
mushroom
and
identified
by
16S
rRNA
gene
sequencing.
The
antibacterial
antibiofilm
activities
cinnamon
essential
oil
(CEO)
on
its
application
mushrooms
were
investigated.
results
indicated
that
CEO
had
a
significant
inhibitory
effect
tolaasii.
After
treatment,
surface
cells
damaged,
leakage
entocyte
observed.
Importantly,
energy
metabolism
inhibited,
with
marked
decrease
ATP
content
ATPase
activity.
crystal
violet
staining
assay
scanning
electron
microscopy
revealed
treatment
not
only
inhibited
biofilm
formation,
but
also
effectively
eradicated
mature
biofilm.
effects
displayed
excellent
maintain
quality
(Agaricus
bisporus).
this
study
will
provide
references
for
further
research
natural
food
agents.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(5), P. 525 - 525
Published: April 28, 2023
Phallus
rubrovolvatus
is
a
unique
mushroom
used
for
medicinal
and
dietary
purposes
in
China.
In
recent
years,
however,
the
rot
disease
of
P.
has
seriously
affected
its
yield
quality,
becoming
an
economically
important
threat.
this
study,
samples
symptomatic
tissues
were
collected,
isolated,
identified
from
five
major
production
regions
Guizhou
Province,
Based
on
combined
analyses
phylogenies
(ITS
EF1-α),
morphological
characteristics
Koch's
postulates,
Trichoderma
koningiopsis
koningii
as
pathogenic
fungal
species.
Among
these,
T.
exhibited
stronger
pathogenicity
than
other
strains;
thus,
was
test
strain
follow-up
experiments.
Upon
co-culturing
with
rubrovolvatus,
hyphae
two
species
intertwined,
color
changed
white
to
red.
Moreover,
wrapped
around
hyphae,
leading
their
shortening
convolution
ultimately
inhibiting
growth
due
wrinkling;
penetrated
entire
basidiocarp
tissue
causing
serious
damage
host
cells.
Further
revealed
that
infection
resulted
swelling
basidiocarps
significantly
enhanced
activity
defense-related
enzymes,
such
malondialdehyde,
manganese
peroxidase,
polyphenol
oxidase.
These
findings
offer
theoretical
support
further
research
mechanisms
fungi
prevention
diseases
caused
by
them.
Journal of Advanced Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 1, 2024
Methyl
jasmonate
(MeJA)
and
MYB
transcription
factors
(TFs)
play
important
roles
in
pathogen
resistance
several
plants,
but
TFs
conjunction
with
MeJA-induced
defense
against
Pseudomonas
tolaasii
edible
mushrooms
remain
unknown.
To
investigate
the
role
of
a
novel
3R-MYB
factor
(AbMYB11)
disease
A.
bisporus
transgenic
Arabidopsis
to
P.
tolaasii.
Mushrooms
were
treated
MeJA
alone
or
combination
phenylpropanoid
pathway
inhibitors,
effects
treatments
on
disease-related
physiological
indicators
determined
assess
inducing
importance
involved.
Subcellular
localization,
gene
expression
analysis,
dual-luciferase
reporter
assay,
electrophoretic
mobility
shift
experiments
performed
elucidate
molecular
mechanism
AbMYB11
regulating
resistance.
application
greatly
improved
mushroom
infection,
suppression
significantly
weakened
this
effect.
treatment
stimulated
accumulation
metabolites,
which
was
accompanied
by
increased
activities
biosynthetic
enzymes
pathway-related
genes
(AbPAL1,
Ab4CL1,
AbC4H1)
an
AbPR-like
gene,
further
confirming
critical
responses
Importantly,
AbMYB11,
localized
nucleus,
rapidly
induced
under
infection;
it
transcriptionally
activated
genes,
overexpression
phenylpropanoid-related
total
phenolics
flavonoids,
This
study
clarified
pivotal
as
regulator
modulating
pathway,
providing
idea
for
breeding
highly
disease-resistant
plants.
Plant Disease,
Journal Year:
2023,
Volume and Issue:
108(2), P. 473 - 485
Published: Sept. 5, 2023
Agaricus
bisporus
(Lange)
Imbach
is
the
most
widely
cultivated
mushroom
in
world.
A.
wet
bubble
disease
one
of
severe
diseases
white
button
mushrooms
and
caused
by
fungal
pathogen
Hypomyces
perniciosus.
The
causes
a
drastic
reduction
yield
because
malformation
deterioration
basidiomes.
However,
mechanism
mushroom’s
development
after
infection
with
H.
perniciosus
remains
obscure.
Therefore,
to
reveal
perniciosus,
interaction
between
host
was
investigated
this
study
using
histopathological,
physiological,
transcriptomic
analyses.
Results
showed
that
irrespective
growth
stages
basidiomes
infected
host’s
malformed
enlarged
mycelia
basidia
indicated
earlier
more
Analyzing
physiological
results
tandem,
we
concluded
mainly
affecting
metabolism
level
phytohormones
(N
6
-isopentenyladenosine,
cis-zeatin,
N
-[delta
2-isopentenyl]-adenine)
fruiting
bodies
rather
than
toxins.
Our
findings
revealed
providing
reference
for
developing
realistic
approaches
control
diseases.
further
clarified
identified
candidate
genes
resistance
breeding.
Additionally,
our
work
provides
valuable
theoretical
basis
technical
support
studying
other
pathogenic
fungi
their
hosts.
