Chemistry & Biodiversity,
Journal Year:
2024,
Volume and Issue:
21(12)
Published: Aug. 22, 2024
Abstract
Structural
modification
is
an
effective
way
to
improve
the
antifungal
activity
of
natural
products
and
has
been
widely
used
in
development
novel
fungicides.
In
this
work,
a
series
aminocoumarin‐based
Schiff
bases
were
synthesized
characterized
by
1
H‐NMR,
13
C
NMR
HR‐MS
spectra.
The
vitro
inhibition
all
compounds
was
tested
against
four
phytopathogenic
fungi
(
Alternaria
solani
,
Fusarium
oxysporum
Botrytis
cinerea
alternata
)
using
mycelial
growth
rate
method.
results
showed
that
most
target
exhibited
significant
activities.
particular,
5b
5c
5d
5h
5n
7c
7n
7p
more
than
commercially
available
fungicides,
chlorothalonil
azoxystrobin.
structure‐activity
relationship
revealed
electron‐withdrawing
groups
with
electronegativity
introduced
at
C‐3
position
improving
inhibitory
halogenated
benzaldehydes
would
be
necessary
preparation
bases.
compound
(EC
50
=8.73
μg/mL)
=26.25
much
better
positive
controls
(chlorothalonil
azoxystrobin).
Therefore,
could
serve
as
promising
lead
for
broad‐spectrum
which
useful
applications
agriculture.
Horticulturae,
Journal Year:
2023,
Volume and Issue:
9(9), P. 983 - 983
Published: Aug. 31, 2023
Decay
caused
by
Neopestalotiopsis
clavispora
is
an
important
postharvest
disease
of
blueberries
that
seriously
affects
the
commercial
value
blueberry
fruit.
In
this
paper,
we
studied
inhibitory
activity
and
mode
action
thymol
against
pathogenic
fungus
clavispora.
The
results
demonstrated
administration
could
limit
mycelial
growth
in
vitro;
effect
was
positively
connected
with
mass
concentrations,
minimal
concentration
(MIC)
100
mg/L.
Further
investigations
revealed
MIC
treatment
dramatically
reduced
germination
spores
led
to
increase
conductivity
pathogen,
leakage
contents,
a
decrease
pH.
Propidium
iodide
(PI)
staining
experiments
damage
cell
membranes.
Additionally,
promoted
mycelium
malondialdehyde
content
accumulation,
inhibited
superoxide
dismutase
(SOD)
catalase
(CAT)
enzyme
activities,
decreased
adenosine
triphosphate
(ATP),
diphosphate
(ADP),
monophosphate
(AMP)
energy
charge
levels,
fluorescence
intensity
significantly
increased
2,7-Dichlorodi-hydrofluorescein
diacetate
(DCFH-DA)
assay.
study
indicate
suppresses
proliferation
compromising
integrity
their
membranes,
promoting
accumulation
cellular
reactive
oxygen
species
(ROS),
interfering
metabolism.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(14), P. 7875 - 7875
Published: July 18, 2024
Direct
barrier
discharge
(DBD)
plasma
is
a
potential
antibacterial
strategy
for
controlling
Fusarium
oxysporum
(F.
oxysporum)
in
the
food
industry.
The
aim
of
this
study
was
to
investigate
inhibitory
effect
and
mechanism
action
DBD
on
F.
oxysporum.
result
curve
shows
that
has
good
inactivation
treatment
severely
disrupted
cell
membrane
structure
resulted
leakage
intracellular
components.
In
addition,
flow
cytometry
used
observe
reactive
oxygen
species
(ROS)
levels
mitochondrial
potential,
it
found
that,
after
treatment,
ROS
accumulation
damage
were
accompanied
by
decrease
antioxidant
enzyme
activity.
results
free
fatty
acid
metabolism
indicate
saturated
content
increased
unsaturated
decreased.
Overall,
led
oxidation
acids,
which
altered
content,
thereby
inducing
damage.
Meanwhile,
plasma-induced
penetrated
accumulated
intracellularly,
leading
collapse
system
ultimately
causing
death.
This
reveals
bactericidal
oxysporum,
provides
possible
control
Chemistry & Biodiversity,
Journal Year:
2024,
Volume and Issue:
21(12)
Published: Aug. 22, 2024
Abstract
Structural
modification
is
an
effective
way
to
improve
the
antifungal
activity
of
natural
products
and
has
been
widely
used
in
development
novel
fungicides.
In
this
work,
a
series
aminocoumarin‐based
Schiff
bases
were
synthesized
characterized
by
1
H‐NMR,
13
C
NMR
HR‐MS
spectra.
The
vitro
inhibition
all
compounds
was
tested
against
four
phytopathogenic
fungi
(
Alternaria
solani
,
Fusarium
oxysporum
Botrytis
cinerea
alternata
)
using
mycelial
growth
rate
method.
results
showed
that
most
target
exhibited
significant
activities.
particular,
5b
5c
5d
5h
5n
7c
7n
7p
more
than
commercially
available
fungicides,
chlorothalonil
azoxystrobin.
structure‐activity
relationship
revealed
electron‐withdrawing
groups
with
electronegativity
introduced
at
C‐3
position
improving
inhibitory
halogenated
benzaldehydes
would
be
necessary
preparation
bases.
compound
(EC
50
=8.73
μg/mL)
=26.25
much
better
positive
controls
(chlorothalonil
azoxystrobin).
Therefore,
could
serve
as
promising
lead
for
broad‐spectrum
which
useful
applications
agriculture.
