Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus DOI Creative Commons
Fan Mu,

Jinsheng Xia,

Jichun Jia

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT Hypovirulence-associated mycoviruses have the potential as biocontrol agents for plant fungal disease management, and exploration of interactions between these phytopathogenic fungi can provide opportunities to elucidate underlying mechanisms hypovirulence antiviruses. We previously found that Sclerotinia sclerotiorum endornavirus 3 (SsEV3), belonging genus Betaendornavirus within family Endornaviridae , confers on fungus but remains unclear. In this study, we SsEV3-infected strain produced fewer sclerotia, failed form infection cushions hosts, exhibited increased cell vacuolation, was more sensitive abiotic stresses. SsEV3 evoked transcriptional rewiring in S. affecting genes related virulence factors pathogenicity RNAi pathway An unknown biological function gene Sssnf1 downregulated following infection. Deletion impaired cushion formation decreased . Five key RNAi-related were significantly upregulated, deletion Ssdcl2 contributed accumulation. Additionally, identified a hypothetical protein encoded by Sshp1 directly interacts with RNA-dependent RNA polymerase (RdRp) domain SsEV3. Although mutants normal colony morphology, they showed higher accumulation reduced resistance reactive oxygen species, indicating similar genes, plays an antiviral role response may represent new antivirus factor. Therefore, examination interaction provides insights into fungi. IMPORTANCE emerged promising agents, studying their helps uncover pathogenesis defense. This study critical its hypovirulence-associated endornavirus, SsEV3, elucidating molecular mycovirus-induced changes not only impairs traits, including sclerotial production also triggers host responses involving typical interference pathways. New factors, such based established system endornavirus. These findings deepen our understanding fungus-mycovirus interactions, highlighting reducing facilitating development mycovirus-based control strategies.

Language: Английский

Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus DOI Creative Commons
Fan Mu,

Jinsheng Xia,

Jichun Jia

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT Hypovirulence-associated mycoviruses have the potential as biocontrol agents for plant fungal disease management, and exploration of interactions between these phytopathogenic fungi can provide opportunities to elucidate underlying mechanisms hypovirulence antiviruses. We previously found that Sclerotinia sclerotiorum endornavirus 3 (SsEV3), belonging genus Betaendornavirus within family Endornaviridae , confers on fungus but remains unclear. In this study, we SsEV3-infected strain produced fewer sclerotia, failed form infection cushions hosts, exhibited increased cell vacuolation, was more sensitive abiotic stresses. SsEV3 evoked transcriptional rewiring in S. affecting genes related virulence factors pathogenicity RNAi pathway An unknown biological function gene Sssnf1 downregulated following infection. Deletion impaired cushion formation decreased . Five key RNAi-related were significantly upregulated, deletion Ssdcl2 contributed accumulation. Additionally, identified a hypothetical protein encoded by Sshp1 directly interacts with RNA-dependent RNA polymerase (RdRp) domain SsEV3. Although mutants normal colony morphology, they showed higher accumulation reduced resistance reactive oxygen species, indicating similar genes, plays an antiviral role response may represent new antivirus factor. Therefore, examination interaction provides insights into fungi. IMPORTANCE emerged promising agents, studying their helps uncover pathogenesis defense. This study critical its hypovirulence-associated endornavirus, SsEV3, elucidating molecular mycovirus-induced changes not only impairs traits, including sclerotial production also triggers host responses involving typical interference pathways. New factors, such based established system endornavirus. These findings deepen our understanding fungus-mycovirus interactions, highlighting reducing facilitating development mycovirus-based control strategies.

Language: Английский

Citations

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