A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

The environmental impact of extracellular matrix preparation DOI Open Access
Ying Chen,

Zihao Ke,

Haiyang Wang

et al.

FEBS Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

The extracellular matrix (ECM) is a network of proteins and other molecules that encase support cells tissues in the body. As clinical biotechnological uses ECM are expanding, it essential to assess environmental impact associated with its production. Due high levels customization, various laboratories employ distinct methods; therefore, this study evaluates three common protocols. Life cycle assessment (LCA) methodology has been developed evaluate impacts products produced through diverse processes. Despite widespread application pharmaceutical industry, LCA seldom utilized estimate effects laboratory This Viewpoint applies functionality via P1, P2, P3. results indicate protocol highest generates approximately 43 times more CO 2 ‐equivalent emissions (CO eq) than lowest impact, while using least impactful demonstrates biocompatibility. Additional indicators such as eutrophication, photochemical oxidation, acidification also vary among tested work underscores need factor development novel biomedical materials.

Language: Английский

Citations

0
Biomedical Application of MSCs in Corneal Regeneration and Repair DOI Open Access
María P. De Miguel, Marta Cadenas-Martín, Martha Stokking

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 695 - 695

Published: Jan. 15, 2025

The World Health Organization estimates that approximately 285 million people suffer from visual impairments, around 5% of which are caused by corneal pathologies. Currently, the most common clinical treatment consists a transplant (keratoplasty) human donor. However, worldwide demand for donor corneas amply exceeds available supply. Lamellar keratoplasty (transplantation replacement only one three layers cornea) is partially solving problem cornea undersupply. Obviously, cell therapy applied to every these will expand current therapeutic options, reducing cost ophthalmological interventions and increasing effectiveness surgery. Mesenchymal stem cells (MSCs) adult with capacity self-renewal differentiation into different lineages. They can be obtained many tissues, such as bone marrow, umbilical cord, adipose tissue, dental pulp, skin, cornea. Their ease collection advantages over embryonic or induced pluripotent make them very practical source experimental potential applications. In this review, we focus on recent advances using MSCs sources replace damaged layers, at both preclinical levels specific diseases.

Language: Английский

Citations

0
Recent advances and prospects of nanoparticle-based drug delivery for diabetic ocular complications DOI Creative Commons
Siqi Wang, Hongyu Yang, Jiaying Zheng

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(8), P. 3551 - 3570

Published: Feb. 25, 2025

Diabetes mellitus (DM) is a chronic metabolic disorder that significantly affects various organ systems. The systemic effects of DM lead to numerous complications, with ocular manifestations being particular concern due their severity and impact on quality life. Hyperglycemia-induced damage often results in range lesions, including diabetic retinopathy (DR), keratopathy, cataracts, glaucoma. These conditions impose considerable physical discomfort patients place substantial economic burden healthcare advent nanotechnology has facilitated the development innovative therapeutic strategies for managing complications. This review highlights several common complications associated DM, focusing pathogenesis treatment strategies. Emphasis placed applications potential treating

Language: Английский

Citations

0
Rational Construct of Extracellular Matrix Mimics via Peptide-Co-assembling Nanofibers for Efficient Bone Regeneration DOI
Xiuhui Wang, Mingkui Shen,

Mengze Ma

et al.

Advanced Fiber Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Biology and medicine on ocular surface restoration: advancements and limits of limbal stem cell deficiency treatments DOI Creative Commons
Vincenzo Giuseppe Genna, Eleonora Maurizi, Paolo Rama

et al.

The Ocular Surface, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

1
Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding DOI Creative Commons
Mehmet Gürdal, Gülinnaz Ercan, Özlem Barut Selver

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1552 - 1552

Published: Nov. 26, 2024

Despite the promising potential of cell-based therapies developed using tissue engineering techniques to treat a wide range diseases, including limbal stem cell deficiency (LSCD), which leads corneal blindness, their commercialization remains constrained. This is primarily attributable limited sources, use non-standardizable, unscalable, and unsustainable techniques, extended manufacturing processes required produce transplantable tissue-like surrogates. Herein, we present first demonstration novel approach combining collagen films (CF), hyaluronic acid (HA), human telomerase-immortalized epithelial cells (T-LESCs), macromolecular crowding (MMC) develop innovative biomimetic substrates for (LESCs). The initial step involved fabrication characterization CF enriched with HA (CF-HA). Subsequently, T-LESCs were seeded on CF, CF-HA, culture plastic (TCP). Thereafter, effect these matrices basic cellular function tissue-specific extracellular matrix (ECM) deposition or without MMC was evaluated. viability metabolic activity cultured TCP found be similar, while CF-HA induced highest (p < 0.05) proliferation. It notable that growth, whereas increased IV, fibronectin, laminin in T-LESC culture. data highlight of, particular, immortalized development substrates, could utilized ocular surface reconstruction following further vitro, vivo, clinical validation approach.

Language: Английский

Citations

1
A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Citations

0