Brief research report: Transcriptional blockade of angiotensin converting enzyme 2 modelled in human retinal pigment epithelial cells DOI Creative Commons
Liam M. Ashander, Amanda L. Lumsden, Yuefang Ma

et al.

Frontiers in Drug Discovery, Journal Year: 2024, Volume and Issue: 4

Published: Oct. 7, 2024

As a key host protein involved in cellular infection by the severe acute respiratory syndrome coronavirus (SARS-CoV-2), angiotensin converting enzyme (ACE)2 is an ideal target for antiviral drugs. Manipulation of transcription provides opportunity graduated blockade that preserves physiological functions. We sought to develop model system evaluating manipulation ACE2 gene using human retinal pigment epithelium. Retinal epithelial cell isolates were prepared from posterior eyecups (n = 11 individual isolates). The cells expressed transcript and protein, expression was not induced hypoxia mimetic dimethyloxaloylglycine, or inflammatory cytokine IL-1β. factors predicted silico cross-referenced with transcriptome, five candidate identified: ETS proto-oncogene 1 factor (ETS1), nuclear I C (NFIC), receptor subfamily 2 group member (NR2C1), TEA domain (TEAD1), zinc finger 384 (ZNF384). candidates individually targeted transfection small interfering (si)RNA. Knockdowns reduced mean all comparison transfected control non-targeted siRNA. Mean under condition NR2C1 knockdown, but ETS1, NFIC, TEAD1, ZNF384 knockdowns. Our findings build on previous work demonstrating potential drugging transcription. Importantly, we show value epithelium as transcriptional blockade, possible approach treating SARS-CoV-2 infection. Brief Research Report.

Language: Английский

Ocular neuroinflammatory response secondary to SARS-CoV-2 infection-a review DOI Creative Commons
Yun Zhao, Ying Tang, Qi Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 4, 2025

With the consistent occurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, prevalence various ocular complications has increased over time. SARS-CoV-2 infection been shown to have neurotropism and therefore lead not only peripheral inflammatory responses but also neuroinflammation. Because receptor for SARS-CoV-2, angiotensin-converting enzyme (ACE2), can be found in many intraocular tissues, disease 2019 (COVID-19) may contribute persistent neuroinflammation, microcirculation dysfunction symptoms. Increased awareness neuroinflammation future research on interventional strategies are important improving long-term outcomes, reducing burden, quality life. Therefore, aim this review is focus discuss current evidence perspectives, especially possible connections between conditions potential treatment strategies.

Language: Английский

Citations

1
Delayed acute retinal necrosis complicated by central retinal artery occlusion post-SARS-CoV-2 recovery: clinical practice and review DOI Creative Commons
Rui Jiang, Dan Lin, Feng Han

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 14, 2025

Language: Английский

Citations

0
A Case of Uveitis after Viral Infection DOI

相宏 万

Asian Case Reports in Emergency Medicine, Journal Year: 2025, Volume and Issue: 13(02), P. 140 - 145

Published: Jan. 1, 2025

Language: Английский

Citations

0
Brief research report: Transcriptional blockade of angiotensin converting enzyme 2 modelled in human retinal pigment epithelial cells DOI Creative Commons
Liam M. Ashander, Amanda L. Lumsden, Yuefang Ma

et al.

Frontiers in Drug Discovery, Journal Year: 2024, Volume and Issue: 4

Published: Oct. 7, 2024

As a key host protein involved in cellular infection by the severe acute respiratory syndrome coronavirus (SARS-CoV-2), angiotensin converting enzyme (ACE)2 is an ideal target for antiviral drugs. Manipulation of transcription provides opportunity graduated blockade that preserves physiological functions. We sought to develop model system evaluating manipulation ACE2 gene using human retinal pigment epithelium. Retinal epithelial cell isolates were prepared from posterior eyecups (n = 11 individual isolates). The cells expressed transcript and protein, expression was not induced hypoxia mimetic dimethyloxaloylglycine, or inflammatory cytokine IL-1β. factors predicted silico cross-referenced with transcriptome, five candidate identified: ETS proto-oncogene 1 factor (ETS1), nuclear I C (NFIC), receptor subfamily 2 group member (NR2C1), TEA domain (TEAD1), zinc finger 384 (ZNF384). candidates individually targeted transfection small interfering (si)RNA. Knockdowns reduced mean all comparison transfected control non-targeted siRNA. Mean under condition NR2C1 knockdown, but ETS1, NFIC, TEAD1, ZNF384 knockdowns. Our findings build on previous work demonstrating potential drugging transcription. Importantly, we show value epithelium as transcriptional blockade, possible approach treating SARS-CoV-2 infection. Brief Research Report.

Language: Английский

Citations

0