Development and Validation of an HPLC-MS/MS Method for the Simultaneous Quantification of Vitexin and Isovitexin in Rabbit Plasma: Pharmacokinetic Insights on a Microcapsule Formulation DOI Creative Commons

Duc Tuan Nguyen,

Trung Nguyen Nguyen Le,

Duc Ngo

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1690 - 1690

Published: April 10, 2025

Vitexin and isovitexin are natural flavone C-glucosides that have numerous benefits for human health. However, their low oral bioavailability poor gastrointestinal absorption dramatically restrict potential medicinal uses. To overcome this challenge, chitosan-coated alginate microcapsules were prepared intragastrical administration to rabbits. An LC-MS/MS method was developed validated the simultaneous determination of vitexin in plasma treated rabbits, using salicylic acid as internal standard. Raw rabbit samples deproteinized acetonitrile a precipitation agent. Chromatographic separation performed on reversed-phase C18 column (100 mm × 4.6 mm, 3.5 µm), with an isocratic mobile solvent system comprising methanol 0.1% acetic (40:60) phase. All analytes standard ionized triple quadrupole mass spectrometer electrospray ionization, operating negative mode multiple reaction monitoring. The analytical approach underwent validation terms suitability, specificity, selectivity, LLOQ 2 ng/mL, linearity (2.0–200 R2 > 0.99), accuracy (the intra- inter-day from 94 110% relative deviations no more than 8.7%, precision recoveries 97% 102%, matrix effect (90–100%), carry-over, dilution integrity (2 times), stability at room frozen temperature up 1 month, all parameters met FDA EMA requirements bioanalytical methods. procedure applied measure encapsulated extracts pilot pharmacokinetic study plasma. Compared raw traditional extracts, enhanced vi-texin regarding Cmax AUC values.

Language: Английский

Development and Validation of an HPLC-MS/MS Method for the Simultaneous Quantification of Vitexin and Isovitexin in Rabbit Plasma: Pharmacokinetic Insights on a Microcapsule Formulation DOI Creative Commons

Duc Tuan Nguyen,

Trung Nguyen Nguyen Le,

Duc Ngo

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1690 - 1690

Published: April 10, 2025

Vitexin and isovitexin are natural flavone C-glucosides that have numerous benefits for human health. However, their low oral bioavailability poor gastrointestinal absorption dramatically restrict potential medicinal uses. To overcome this challenge, chitosan-coated alginate microcapsules were prepared intragastrical administration to rabbits. An LC-MS/MS method was developed validated the simultaneous determination of vitexin in plasma treated rabbits, using salicylic acid as internal standard. Raw rabbit samples deproteinized acetonitrile a precipitation agent. Chromatographic separation performed on reversed-phase C18 column (100 mm × 4.6 mm, 3.5 µm), with an isocratic mobile solvent system comprising methanol 0.1% acetic (40:60) phase. All analytes standard ionized triple quadrupole mass spectrometer electrospray ionization, operating negative mode multiple reaction monitoring. The analytical approach underwent validation terms suitability, specificity, selectivity, LLOQ 2 ng/mL, linearity (2.0–200 R2 > 0.99), accuracy (the intra- inter-day from 94 110% relative deviations no more than 8.7%, precision recoveries 97% 102%, matrix effect (90–100%), carry-over, dilution integrity (2 times), stability at room frozen temperature up 1 month, all parameters met FDA EMA requirements bioanalytical methods. procedure applied measure encapsulated extracts pilot pharmacokinetic study plasma. Compared raw traditional extracts, enhanced vi-texin regarding Cmax AUC values.

Language: Английский

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