Optimizing Ge Enrichment in Lyophyllum decastes Fermentation for Enhanced Biological Activity DOI Creative Commons

Sher Hassan,

Jinyan Zhao,

Sana Tahir

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 641 - 641

Published: Dec. 14, 2024

This study enhanced germanium (Ge) enrichment in the liquid fermentation of edible fungus Lyophyllum decastes order to boost its biological activity, particularly antioxidant and immunomodulatory properties. Through use single-factor Plackett–Burman designs, experiments revealed critical parameters affecting Ge enrichment, including oxide concentration, potato powder peptone levels. The optimization Box–Behnken response surface methodology resulted a concentration 3.61 mg/L, significantly enhancing biomass, protein, polysaccharide, flavonoid content mycelium. In contrast traditional fermentation, Ge-rich mycelial biomass by 30.97% elevated organic 50.19-fold. An analysis antioxidants that Ge-enriched water extract exhibited heightened augmenting TNF-α production RAW264.7 cells 73.29% at 200 μg/g. These findings indicate L. holds promise for functional applications health-supportive products due robust immune-enhancing capabilities.

Language: Английский

Identification of an arabinogalactan with special structure from Moringa Oleifera leaf and exploration of its immunomodulatory activity DOI
Jinyan Gu,

Xinyi Yang,

Lianzhu Lin

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 134616 - 134616

Published: Aug. 8, 2024

Language: Английский

Citations

4
Structural Characterization of Foxtail Millet (Setaria italica) Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities DOI Creative Commons
Haiying Zhang,

Chengyu Peng,

Wei Zhang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 113 - 113

Published: Jan. 20, 2025

A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation column chromatography. Size-Exclusion Chromatography–Multi-Angle Light Scattering–Refractive Index (SEC-MALLS-RI) high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly neutral exclusively composed of glucose as the sugar unit, with molecular weight 14.823 kDa. The structural characterization results obtained gas chromatography–mass spectrometer (GC–MS) nuclear magnetic resonance spectroscopy (NMR) spectra suggest primarily consists main chain linked by →4)-α-D-Glcp-(1→ minor quantities →4,6)-α-D-Glcp-(1→ to form chain, branching mainly α-D-Glcp-(1→ attached O-6 position residues. Based on these findings, antioxidant immunomodulatory activities were evaluated in vitro. indicated exhibited moderate 2, 2-diphenyl-1-picrylhydrazyl (DPPH) 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical scavenging capacity total (TAOC). Furthermore, stimulated macrophage proliferation inhibited production nitric oxide (NO) inflammatory factors (TNF-α, IL-1β, IL-6) lipopolysaccharide (LPS)-stimulated RAW 246.7 cells. Overall, findings this study holds promise potential agent immunologic substance functional foods.

Language: Английский

Citations

0
Structural determination, immunomodulatory activity, and antitumor activity of a low-molecular-weight polysaccharide extracted from Lepista sordida DOI
Xinlin Li, Hui Zhao, Peng Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 307, P. 141973 - 141973

Published: March 11, 2025

Language: Английский

Citations

0
Dictyophora-based evaporator developed by sericin bidirectional crosslinking for photocatalytic degradation and efficient solar desalination in harsh environments DOI
Qian Yang, Junkai Gao,

Mengsheng Xia

et al.

Desalination, Journal Year: 2025, Volume and Issue: unknown, P. 118849 - 118849

Published: April 1, 2025

Language: Английский

Citations

0
Effects of ultrasonic enhancement on physicochemical properties, structural, antioxidant activity and potential antidiabetic effect of asparagus juice DOI Creative Commons

Tianyu Kong,

Fatao He,

En Shi

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117767 - 117767

Published: April 1, 2025

Language: Английский

Citations

0
Non-starch polysaccharides from nutmeg: Preparation process and bioactivity DOI
Zhenlei Wang, Wen‐Chih Cheng, Zitong Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143341 - 143341

Published: April 18, 2025

Language: Английский

Citations

0
Effect of hot-air drying processing on the volatile organic compounds and maillard precursors of Dictyophora Rubrovalvata based on GC-IMS, HPLC and LC-MS DOI
Lingshuai Meng, Yu Nie,

Qing‐Song Zhou

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141074 - 141074

Published: Aug. 31, 2024

Language: Английский

Citations

2
Isolation, structural characterization, and hypoglycemic activity of polysaccharides from the stems of Panax ginseng C. A. Meyer DOI Creative Commons
Weiwei Xu, Zhou We, Jinfeng Sun

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 8

Published: Nov. 1, 2024

The purpose of this study was to obtain polysaccharides from Panax ginseng C. A. Meyer stems (PGSPs), agro-byproducts with development potential, and fully explore the potential value in P. stems. Two novel firstly (PGSP-1 PGSP-2) were obtained by water extraction alcohol precipitation method column chromatography, then characterized FT-IR, HPGPC, HPLC, SEM, TGA, GC–MS NMR. results demonstrated that PGSP-1 (Mw = 723.83 kDa) PGSP-2 620.48 →4)- β -D-Gal p -(1→, →6)- α -D-Glc -(1 → →2)- -L-Rha as skeleton, →4,6)- →2,6)- -D-Man cross junction, -L-Araf-(1 terminal unit, still contained , while -D-Xyl →3)- A-(1→, A-(1 →) different microstructures thermal stability. And hypoglycemic activity revealed both showed excellent inhibitory against -glucosidase -amylase, which had better performance. inhibition kinetics result PGSPs on -amylase non-competitive type mixed inhibition. This provided a theoretical basis for making full use exploiting economic such offered reference effective utilization PGSP functional component prevent reduce T2DM.

Language: Английский

Citations

1
Optimizing Ge Enrichment in Lyophyllum decastes Fermentation for Enhanced Biological Activity DOI Creative Commons

Sher Hassan,

Jinyan Zhao,

Sana Tahir

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 641 - 641

Published: Dec. 14, 2024

This study enhanced germanium (Ge) enrichment in the liquid fermentation of edible fungus Lyophyllum decastes order to boost its biological activity, particularly antioxidant and immunomodulatory properties. Through use single-factor Plackett–Burman designs, experiments revealed critical parameters affecting Ge enrichment, including oxide concentration, potato powder peptone levels. The optimization Box–Behnken response surface methodology resulted a concentration 3.61 mg/L, significantly enhancing biomass, protein, polysaccharide, flavonoid content mycelium. In contrast traditional fermentation, Ge-rich mycelial biomass by 30.97% elevated organic 50.19-fold. An analysis antioxidants that Ge-enriched water extract exhibited heightened augmenting TNF-α production RAW264.7 cells 73.29% at 200 μg/g. These findings indicate L. holds promise for functional applications health-supportive products due robust immune-enhancing capabilities.

Language: Английский

Citations

1