Combined fermentation and ARTP mutagenesis to enhance lipase activity of Penicillium camembertii and its application for high-purity 1,3-diacylglycerol preparation

Food Chemistry, Journal Year: 2024, Volume and Issue: 465, P. 142072 - 142072

Published: Nov. 16, 2024

Language: Английский

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Preparation and Biochemical Characterization of Penicillium crustosum Thom P22 Lipase Immobilization Using Adsorption, Encapsulation, and Adsorption–Encapsulation Approaches

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 434 - 434

Published: Jan. 21, 2025

This work describes the immobilization and characterization of purified Penicillium crustosum Thom P22 lipase (PCrL) using adsorption, encapsulation, adsorption–encapsulation approaches. The maximum activity immobilized PCrL on CaCO3 microspheres sodium alginate beads was shifted from 37 to 45 °C, compared with that free enzyme. When coupled zeolite or chitosan, yield reached 100% showed improved stability over a wide temperature range, retaining all its initial after one-hour incubation at 60 °C. significantly improves catalytic performance in organic solvents, pH tolerance value, thermal stability. Interestingly, 95% almost 50% PCrL’s retained 6 12 cycles, respectively. characteristics forms were analyzed by X-ray diffraction scanning electron microscopy combined energy dispersive spectroscopy. conversion efficiency oleic acid methanol methyl esters (biodiesel), CaCO3, 65% h 40 while generated only 30% conversion, under same conditions.

Language: Английский

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Selective production of functional sn-1,3-diacylglycerol by microbial lipases: A comprehensive review

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144017 - 144017

Published: March 1, 2025

Language: Английский

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Effect of m-PEG modification on the properties and structure of recombinant lipase from Aspergillus niger GZUF36

Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 104258 - 104258

Published: May 3, 2024

Language: Английский

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