Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 DOI Creative Commons

Siti Soleha,

Syarifah Syarifah,

Tito Nurseha

et al.

INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, Journal Year: 2024, Volume and Issue: 6(2), P. 121 - 130

Published: Oct. 28, 2024

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization lipase coding necessary for large-scale production through genetic engineering. This study aimed to observe molecular weight, amino acid sequence, length, conserved acids in DNA gene, with goal identifying characterizing lipase-coding SBE01. The primer design process was conducted amplify using specialized software sequence alignment phylogenetic analysis. Amplification carried out PCR designed primer, forward (GTC ATG TAC TTC CAY GGN GG), reverse (GGT TGC CGC CGG CDS WRT CNC C). under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles denaturation 95°C, annealing 66°C (30 seconds), 70°C elongations (1 minute) final elongation (10 minutes). results were electrophoresed 1% agarose gel a 1 kb marker. sequenced analyzed sequences type expressed. Sequencing resulted 387 bp nucleotide sequence. had high homology strain TA144. encodes protein consisting 129 contains HGG (His-Gly-Gly) motif, which characteristic lipases family IV, also known as hormone-sensitive (HSL) family. suggests that shares structural functional similarities other enzymes HSL family, playing key role lipid metabolism.

Language: Английский

Ultrasound assisted deep eutectic solvent-based extraction of Montepulciano d’ Abruzzo grape seeds for the recovery of the grape seed oil and its biological evaluation DOI Creative Commons

Lorenza Marinaccio,

Giulia Gentile,

Gökhan Zengin

et al.

Food Chemistry X, Journal Year: 2025, Volume and Issue: 26, P. 102273 - 102273

Published: Feb. 1, 2025

Grape seeds are by-products of vinification process. In this work, a green ultrasound-assisted extraction grape oil was performed by using the natural volatile deep eutectic solvent (NADES) menthol: thymol 1:1. The obtained compared to that deriving from UAE with n-hexane in terms fatty acids composition and biological activities. content linoleic acid low for DES extracted oil; linolenic increased 0.53 % 5.18 %. showed best total phenolic (18.65 mg GAE/g) flavonoid (0.73 RE/g) contents highest results FRAP, CUPRAC, MCA Phosphomolybdenum assays. also higher amylase inhibition (0.57 mmol ACAE/g) than extract (0.47 ACAE/g). Finally, anti-inflammatory activity assessed vivo through tree different assays, suggesting their could be partially responsible significant effect seed NADES.

Language: Английский

Citations

1
The current research status of immobilized lipase performance and its potential for application in food are developing toward green and healthy direction: A review DOI
Ning Wang,

Weizhe Wang,

Yufeng Su

et al.

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Abstract Immobilized lipases have received great attention in food, environment, medicine, and other fields due to their easy separation, high stability (temperature, pH), storage properties. After immobilization, lipase transforms from a homogeneous heterogeneous state, making it easier recover the reaction substrate achieve recycling, which is line with concept of green chemistry reduces protein contamination product. There are various materials for enzyme including polysaccharides natural sources, inorganic compounds, carbon nanotubes, metal–organic framework materials, so forth. Magnetic immobilization carriers been widely studied ability separation by adding magnetic field. Its method can be simply divided into two categories: physical action (adsorption, embedding) chemical binding (covalent, cross‐linking). Some studies mainly discuss support methods, applications immobilized food. On this basis, our review also focuses on changes crosslinking agents lipases, different methods promote new trends study proposes prospects research food industry.

Language: Английский

Citations

0
Protective effects of capric acid-rich medium-long-medium chain type structured lipids against metabolic abnormalities in mice fed a high-fat diet DOI

Angie Vanessa Caicedo-Paz,

Camila Farías, Camila Cisternas

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106232 - 106232

Published: Feb. 1, 2025

Language: Английский

Citations

0
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 DOI Creative Commons

Siti Soleha,

Syarifah Syarifah,

Tito Nurseha

et al.

INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, Journal Year: 2024, Volume and Issue: 6(2), P. 121 - 130

Published: Oct. 28, 2024

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization lipase coding necessary for large-scale production through genetic engineering. This study aimed to observe molecular weight, amino acid sequence, length, conserved acids in DNA gene, with goal identifying characterizing lipase-coding SBE01. The primer design process was conducted amplify using specialized software sequence alignment phylogenetic analysis. Amplification carried out PCR designed primer, forward (GTC ATG TAC TTC CAY GGN GG), reverse (GGT TGC CGC CGG CDS WRT CNC C). under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles denaturation 95°C, annealing 66°C (30 seconds), 70°C elongations (1 minute) final elongation (10 minutes). results were electrophoresed 1% agarose gel a 1 kb marker. sequenced analyzed sequences type expressed. Sequencing resulted 387 bp nucleotide sequence. had high homology strain TA144. encodes protein consisting 129 contains HGG (His-Gly-Gly) motif, which characteristic lipases family IV, also known as hormone-sensitive (HSL) family. suggests that shares structural functional similarities other enzymes HSL family, playing key role lipid metabolism.

Language: Английский

Citations

0