Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study DOI Creative Commons

Sepide Ali Ashrafi,

Milad Asadi, Dariush Shanehbandi

et al.

BMC Neurology, Journal Year: 2022, Volume and Issue: 22(1)

Published: Nov. 3, 2022

MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity autoimmune disorders, such as multiple sclerosis (MS).This study was conducted investigate changed expression pattern miRNA-145 and miRNA-155 in MS.We collected blood samples 75 patients with relapsing-remitting MS healthy controls. Ficoll-Hypaque density gradient method used isolate peripheral mononuclear cells. Also, total RNA extracted subjected RT-PCR analysis. We the Mann-Whitney U test evaluate differences levels target miRNAs between groups.We found that (P = 0.012) 0.005) were partly reduced relapse-remitting comparison The had an area under curve (AUC) 0.621 0.01) AUC 0.625 0.008).Decreased development MS, while further large scale observational studies meta-analyses required.

Language: Английский

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13 DOI
Zhen Zhang, Kaiping Deng,

Ziqi Kang

et al.

The FASEB Journal, Journal Year: 2022, Volume and Issue: 36(7)

Published: May 30, 2022

MicroRNAs (miRNAs) are evolutionarily conserved endogenous small non-coding RNAs that play critical roles in skeletal muscle development. In this study, we identified putative miRNAs were differentially expressed the longissimus dorsi between fetus (75 days of pregnancy) and lamb (1 day age). We detected 1208 miRNAs, 313 which expressed. particular, found miR-145-5p was highly muscle. addition, our results demonstrated overexpression inhibited differentiation apoptosis goat primary myoblasts (GPMs), whereas knockdown had opposite effect. The coding domain sequence (CDS) ubiquitin-specific peptidase 13 (USP13) predicted validated as a target miR-145-5p. also influence key regulator GPMs is primarily mediated by targeting inhibiting USP13. Taken together, these revealed novel pathway development targets CDS region USP13 to regulate GPMs.

Language: Английский

Citations

11
Functional Relevance of the Long Intergenic Non-Coding RNA Regulator of Reprogramming (Linc-ROR) in Cancer Proliferation, Metastasis, and Drug Resistance DOI Creative Commons

José A. Peña-Flores,

Diego Enríquez-Espinoza,

Daniela Muela‐Campos

et al.

Non-Coding RNA, Journal Year: 2023, Volume and Issue: 9(1), P. 12 - 12

Published: Jan. 31, 2023

Cancer is responsible for more than 10 million deaths every year. Metastasis and drug resistance lead to a poor survival rate are major therapeutic challenge. Substantial evidence demonstrates that an increasing number of long non-coding RNAs dysregulated in cancer, including the intergenic RNA, regulator reprogramming (linc-ROR), which mostly exerts its role as onco-lncRNA acting competing endogenous RNA sequesters micro RNAs. Although properties linc-ROR relation some cancers have been reviewed past, active research appends constantly better comprehension different stages cancer. Moreover, molecular details recent papers omitted or partially reported, thus importance this review aimed contribute up-to-date understanding implication cancer tumorigenesis, progression, metastasis, chemoresistance. As involvement elucidated, improvement diagnostic prognostic tools could promote advance targeted specific therapies precision oncology.

Language: Английский

Citations

5
A miRNA-Based Prognostic Model to Trace Thyroid Cancer Recurrence DOI Open Access
Eman A. Toraih, Manal S. Fawzy, Bo Ning

et al.

Cancers, Journal Year: 2022, Volume and Issue: 14(17), P. 4128 - 4128

Published: Aug. 26, 2022

Papillary thyroid carcinomas (PTCs) account for most endocrine tumors; however, screening and diagnosing the recurrence of PTC remains a clinical challenge. Using microRNA sequencing (miR-seq) to explore miRNA expression profiles in tissues adjacent normal tissues, we aimed determine which miRNAs may be associated with metastasis. Public databases such as TCGA GEO were utilized data sourcing external validation, respectively, miR-seq results validated using quantitative real-time PCR (qRT-PCR). We found miR-145 significantly downregulated tumor blood. Deregulation was related clinicopathological features patients including size, lymph node metastasis, TNM stage, recurrence. In silico analysis showed that can negatively regulate multiple genes TC signaling pathway cell apoptosis, proliferation, stem differentiation, angiogenesis, Taken together, current study suggests biomarker Further mechanistic studies are required uncover its cellular roles this regard.

Language: Английский

Citations

9
LncRNA PTOV1-AS2 Promotes Colon Cancer Progression through the miR-145-5p/FSCN1 Axis DOI Open Access
Zhen Zhang, Honglei Wu, Zhaosheng Chen

et al.

Journal of Oncology, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 12

Published: March 14, 2023

Objective. The long noncoding RNA (lncRNA) gene PTOV1-AS2 is a potentially oncogenic lncRNA gene. However, its role and regulatory mechanism in the occurrence development of colon cancer are still unclear. In this study, was used as starting point to investigate competing endogenous (ceRNA) mechanisms cancer. Methods. expression mRNA tissues cell lines measured by quantitative real-time polymerase chain reaction (qRT-PCR) screened for differential cells. We examined effects overexpression or downregulation on various cellular processes HCT116 SW620 cells after transfection with an construct PTOV1-AS2p-specific shRNA, respectively. particular, we proliferation, migration, invasion using counting kit-8 CCK-8 assay Transwell migration assays, addition, binding targets PTOV1-AS2/miR-145-5p miR-145-5p/FSCN1 were predicted bioinformatics tools validated dual luciferase assay. also effect axis FSCN1 qRT-PCR analysis. Furthermore, investigated PTOV1-AS2/miR-145-5p/FSCN1 biological function vitro model reduced simultaneous miR-145-5p inhibitor vector. Additionally, established xenograft tumor nude mouse it locally injected vector growth survival status tumor-bearing mice. Results. found that highly expressed cancer, which associated worse survival. High promoted invasion, while low inhibited these microRNA bind 3′-UTR region both FSCN1. decreased protein target PTOV1-AS2-induced lines. Also, silencing enhancing could partially restore inhibition tumorigenic capacity caused vivo. Conclusion. promotes progression “sponging” upregulate

Language: Английский

Citations

4
Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study DOI Creative Commons

Sepide Ali Ashrafi,

Milad Asadi, Dariush Shanehbandi

et al.

BMC Neurology, Journal Year: 2022, Volume and Issue: 22(1)

Published: Nov. 3, 2022

MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity autoimmune disorders, such as multiple sclerosis (MS).This study was conducted investigate changed expression pattern miRNA-145 and miRNA-155 in MS.We collected blood samples 75 patients with relapsing-remitting MS healthy controls. Ficoll-Hypaque density gradient method used isolate peripheral mononuclear cells. Also, total RNA extracted subjected RT-PCR analysis. We the Mann-Whitney U test evaluate differences levels target miRNAs between groups.We found that (P = 0.012) 0.005) were partly reduced relapse-remitting comparison The had an area under curve (AUC) 0.621 0.01) AUC 0.625 0.008).Decreased development MS, while further large scale observational studies meta-analyses required.

Language: Английский

Citations

7