Unraveling Therapeutic Opportunities and the Diagnostic Potential of microRNAs for Human Lung Cancer

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2061 - 2061

Published: July 31, 2023

Lung cancer is a major public health problem and leading cause of cancer-related deaths worldwide. Despite advances in treatment options, the five-year survival rate for lung patients remains low, emphasizing urgent need innovative diagnostic therapeutic strategies. MicroRNAs (miRNAs) have emerged as potential biomarkers targets due to their crucial roles regulating cell proliferation, differentiation, apoptosis. For example, miR-34a miR-150, once delivered via liposomes or nanoparticles, can inhibit tumor growth by downregulating critical promoting genes. Conversely, miR-21 miR-155, frequently overexpressed cancer, are associated with increased invasion, chemotherapy resistance. In this review, we summarize current knowledge miRNAs carcinogenesis, especially those induced exposure environmental pollutants, namely, arsenic benzopyrene, which account up 1/10 cases. We then discuss recent miRNA-based therapeutics diagnostics. Such information will provide new insights into pathogenesis modalities based on miRNAs.

Language: Английский

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Gold Nanoparticles Downregulate IL-6 Expression/Production by Upregulating microRNA-26a-5p and Deactivating the RelA and NF-κBp50 Transcription Pathways in Activated Breast Cancer Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1404 - 1404

Published: Jan. 24, 2024

MicroRNAs (miRNAs) are involved in the modulation of pathogenic genes by binding to their mRNA sequences’ 3′ untranslated regions (3′UTR). Interleukin-6 (IL-6) is known promote cancer progression and treatment resistance. In this study, we aimed explore therapeutic effects gold nanoparticles (GNP) against IL-6 overexpression miRNA-26a-5p breast (BC) cells. GNP were synthesized using trisodium citrate method characterized through UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM). To predict miR-26a-5p mRNA’s 3′UTR, utilized bioinformatics algorithms. Luciferase reporter clone assays anti-miRNA-26a-5p transfection employed validate miR26a-5p 3′UTR. The activity RelA NF-κBp50 was assessed confirmed Bay 11-7082. spherical with a mean size 28.3 nm, exhibiting high stability, suitable for BC cell treatment. We found that directly regulated MCF-7 cells activated PMA. Treatment resulted inhibition secretion increase miR26a-5p. Furthermore, deactivated NF-κBp65/NF-κBp50 transcription activity. newly engineered demonstrated safety showed promise as approach reducing overexpression. suppressed deactivating upregulating expression These findings suggest have potential intervention targeting associated pathways.

Language: Английский

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From sweat to hope: The role of exercise‐induced extracellular vesicles in cancer prevention and treatment

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(8)

Published: Aug. 1, 2024

Abstract The benefits of regular physical exercise on cancer prevention, as well reducing fatigue, treatment side effects and recurrence, improving quality life overall survival patients, are increasingly recognised. Initial studies showed that the concentration extracellular vesicles (EVs) increases during activity EVs carry biologically active cargo. These released by blood cells, skeletal muscle other organs involved in exercise, thus suggesting may mediate tissue crosstalk exercise. This possibility triggered a great interest study roles systemic adaptation to their potential applications prevention various diseases, including cancer. review presents exploring molecular cargo Furthermore, we discuss putative stimuli trigger EV release from cell types, biological functions impact exercise‐induced development progression. Understanding interplay between EVs, biology offer insights into novel therapeutic strategies preventive measures for

Language: Английский

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Hypoxia-Preconditioned Adipose Stem Cell Exosomes Promote Adipose Graft Vascular Regeneration via miRNA-126

Aesthetic Plastic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Language: Английский

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Multiplexed Molecular Endophenotypes Help Identify Hub Genes in Non-Small Cell Lung Cancer: Unlocking Next-Generation Cancer Phenomics

OMICS A Journal of Integrative Biology, Journal Year: 2025, Volume and Issue: 29(1), P. 8 - 17

Published: Jan. 1, 2025

Next-generation cancer phenomics by deployment of multiple molecular endophenotypes coupled with high-throughput analyses gene expression offer veritable opportunities for triangulation discovery findings in non-small cell lung (NSCLC) research. This study reports differentially expressed genes NSCLC using publicly available datasets (GSE18842 and GSE229253), uncovering 130 common that may potentially represent crucial signatures NSCLC. Additionally, network GeneMANIA STRING revealed significant coexpression interaction patterns among these genes, four notable hub genes-GRK5, CAV1, PPARG, CXCR2-identified as pivotal progression. Validation indicated their consistent downregulation tumor tissues compared to normal counterparts. Gene across the representing pathological stages distinct trends, emphasizing putative roles biomarkers Moreover, three miRNAs (hsa-miR-429, hsa-miR-335-5p, hsa-miR-126-3p) showed strong associations while SREBF1 emerged a relevant transcription factor. Pathway enrichment analysis identified chemokine signaling pathway significantly associated highlighting its role progression immune evasion. Cell-type endothelial cells play pathogenesis. Finally, survival demonstrated GRK5 is potential oncogenic marker, whereas CAV1 have protective effect. These collectively underscore critical interactions suggest novel paths translational research, targeted therapies, prognostic markers clinical settings. They also attest promises next-generation findings.

Language: Английский

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The Emerging Role of MicroRNAs in Nasal Inflammatory Diseases and Tumors: From Bench to Bedside

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 295 - 295

Published: Feb. 28, 2025

Background/Objectives: MicroRNAs (miRNAs) play a crucial role in regulating immune responses and have been implicated the pathogenesis of various nasal diseases, including chronic rhinosinusitis (CRS), allergic rhinitis (AR), sinonasal tumors. This review comprehensively explores emerging miRNAs inflammatory oncological highlighting their diagnostic, prognostic, therapeutic potential. Methods: A comprehensive literature was conducted to summarize current findings on miRNA expression conditions Key studies evaluating miRNA-mediated regulatory mechanisms, potential biomarker applications, approaches were analyzed. Results: Altered profiles contribute CRS, AR, Specific miRNAs, such as miR-125b miR-155 are upregulated CRS promoting inflammation tissue remodeling. In tumors, dysregulated miR-126 miR-34/miR-449 clusters, influence tumor progression response. Exosome-mediated delivery emerges promising avenue for precision medicine, offering novel strategies miRNA-based diagnostics therapies. Conclusions: key regulators with applications non-invasive targeted Further research into interventions may improve treatment outcomes development personalized medicine disorders malignancies.

Language: Английский

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Amogel: a multi-omics classification framework using associative graph neural networks with prior knowledge for biomarker identification

BMC Bioinformatics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 28, 2025

The advent of high-throughput sequencing technologies, such as DNA microarray and sequencing, has enabled effective analysis cancer subtypes targeted treatment. Furthermore, numerous studies have highlighted the capability graph neural networks (GNN) to model complex biological systems capture non-linear interactions in data. GNN proven be useful leveraging multiple types omics data, including prior knowledge from various sources, transcriptomics, genomics, proteomics, metabolomics, improve classification. However, current works do not fully utilize learning potential lack integration ability analyse multi-omics data simultaneously with knowledge. Nevertheless, relying on limited generating gene graphs might lead less accurate classification due undiscovered significant gene-gene interactions, which may require expert intervention can time-consuming. Hence, this study proposes a called associative embedding (AMOGEL) effectively integrate datasets through coupled association rule mining (ARM). AMOGEL employs an early fusion technique using ARM mine intra-omics inter-omics relationships, forming synthetic information before training. Moreover, introduces multi-dimensional edges, associations or edges main contributors auxiliary contributors. Additionally, it uses ranking based attention scores, considering relationships between neighbouring genes. Several experiments were performed BRCA KIPAN demonstrate (miRNA, mRNA, methylation) protein-protein KEGG pathways Gene Ontology. experimental results showed that outperformed state-of-the-art models terms accuracy, F1 score AUC score. findings represent crucial step forward advancing subtype

Language: Английский

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miR-126: a bridge between cancer and exercise

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 15, 2025

The microRNA miR-126 supports endothelial cells and blood vessel integrity. Recent research has shown that it also serves as a key link between exercise cancer. This article delves into how affects the expression of miR-126, impacting cardiovascular well-being metabolic control. examines various contributions in cancer, acting both suppressor an enhancer depending on particular context. Regular aerobic exercises, including HIIT, consistently increase levels leading to enhanced angiogenesis, repair, improved vascular function through mechanisms involving VEGF, HIF-1α, EPC mobilization. Resistance training similar pathways, but does not cause significant change levels.MiR-126 involves cancer by suppressing tumor growth controlling pathways such PI3K/Akt, ERK/MAPK, EMT. Lower are associated with negative outcomes, later stages disease, increased spread different types like glioblastoma, CRC, ovarian, esophageal, gastric, prostate cancer.The relationship suggests possible therapeutic approach, where regulation could help improve slow growth. Further studies should focus understanding specific molecular which connects these areas, potential interventions utilize its regulatory network promote enhance treatment.

Language: Английский

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MicroRNA in cancer therapy: breakthroughs and challenges in early clinical applications

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: April 21, 2025

Abstract MicroRNAs (miRNAs) have emerged as pivotal regulators in cancer biology, influencing tumorigenesis, progression, and resistance to therapy. Their ability modulate multiple oncogenic tumor-suppressive pathways positions them promising therapeutic tools or targets. This review examines the dual role of miRNAs solid hematological malignancies, starting from their dysregulation various types. Therapeutic approaches, including miRNA replacement inhibition strategies, are discussed alongside innovative delivery systems such lipid nanoparticles exosomes. Despite transformative potential, challenges persist, off-target effects, immune activation, inefficiencies. Recent clinical trials demonstrate both progress hurdles, underscoring need for advanced strategies optimize specificity minimize toxicity. provides an updated comprehensive overview current landscape miRNA-based therapies under early investigation explores future directions integrating these approaches into precision oncology.

Language: Английский

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Crosstalk Between m6A RNA Methylation and miRNA Biogenesis in Cancer: An Unholy Nexus

Molecular Biotechnology, Journal Year: 2023, Volume and Issue: 66(11), P. 3042 - 3058

Published: Oct. 13, 2023

Language: Английский

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