Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 7, 2024
Language: Английский
Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 40(1)
Published: June 5, 2024
Abstract Many types of gynecological cancer (GC) are often silent until they reach an advanced stage, and therefore diagnosed too late for effective treatment. Hence, there is a real need more efficient diagnosis treatment patients with GC. During recent years, researchers have increasingly studied the impact microRNAs development, leading to number applications in detection MicroRNAs particular group tiny RNA molecules that regulate regular gene expression by affecting translation process. The downregulation numerous miRNAs has been observed human malignancies. Let-7 example miRNA controls cellular processes as well signaling cascades affect post-transcriptional expression. Recent research supports hypothesis enhancing let-7 those cancers where it downregulated may be potential option. Exosomes vesicles move through body fluids can include components like (including let-7) important communication between cells. Studies proved exosomes able enhance tumor growth, angiogenesis, chemoresistance, metastasis, immune evasion, thus suggesting their importance GC management. Graphical Impact on female malignancies diseases reproductive tract. dysregulated variety gynaecological obstetric disorders.
Language: Английский
Citations
5
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: Jan. 25, 2024
Abstract Background Lymph node metastasis (LNM) is a prognostic biomarker and affects therapeutic selection in colorectal cancer (CRC). Current evaluation methods are not adequate for estimating LNM CRC. H&E images contain much pathological information, collagen also the biological behavior of tumor cells. Hence, objective study to investigate whether fully quantitative pathomics-collagen signature (PCS) microenvironment can be used predict LNM. Methods Patients with histologically confirmed stage I-III CRC who underwent radical surgery were included training cohort (n = 329), internal validation external 315). Fully pathomics features extracted from digital multiphoton specimens, respectively. LASSO regression was utilized develop PCS. Then, PCS-nomogram constructed incorporating PCS clinicopathological predictors cohort. The performance evaluated via calibration, discrimination, clinical usefulness. Furthermore, tested cohorts. Results By regression, developed based on 11 9 features. A significant association found between three cohorts ( P < 0.001). PCS, preoperative CEA level, lymphadenectasis CT, venous emboli and/or lymphatic invasion perineural (VELIPI), pT achieved AUROCs 0.939, 0.895, 0.893 calibration curves identified good agreement nomogram-predicted actual outcomes. Decision curve analysis indicated that clinically useful. Moreover, still an independent predictor at station Nos. 1, 2, 3. nomogram displayed 0.849–0.939 cohort, 0.837–0.902 0.851–0.895 nodal stations. Conclusions This proposed integrating significantly associated LNM, has potential useful tool predicting individual patients.
Language: Английский
Citations
4
Cellular Signalling, Journal Year: 2024, Volume and Issue: 117, P. 111101 - 111101
Published: Feb. 15, 2024
Language: Английский
Citations
4
Technology in Cancer Research & Treatment, Journal Year: 2024, Volume and Issue: 23
Published: Jan. 1, 2024
Circular RNAs (circRNAs) serve a crucial regulatory role in ovarian cancer (OC). RNA ArfGAP with FG repeats 1 (circAGFG1) has been shown to be involved promoting the progression of several cancers, containing triple-negative breast cancer, esophageal and colorectal cancer. However, function circAGFG1 OC is unclear.
Language: Английский
Citations
4
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 24, 2025
Abstract Nasopharyngeal carcinoma (NPC) is an Asia‐prevalent malignancy, yet its genetic underpinnings remain incompletely understood. Here, a transcriptome‐wide association study (TWAS) conducted on NPC, leveraging gene expression prediction models based epithelial tissues and genome‐wide (GWAS) summary statistics from 1577 NPC cases 6359 controls of southern Chinese descent. The TWAS identifies VAMP8 chromosome 2p11.2 as novel susceptibility for NPC. Further fine‐mapping analyses pinpoint rs1058588, located within , causal variant through eQTL colocalization, GWAS across multiple cohorts, achieving significance (OR = 1.18, P 3.09 × 10 −10 ). Functional assays demonstrate that exerts tumorigenic role in enhancing cell proliferation, migration, tumor growth. Mechanically, it uncovered rs1058588 modulates by altering binding affinity to miR‐185 . Furthermore, the results show interacts with DHX9 facilitate nuclear recruitment p65, activating NF‐κB pathway. Collectively, findings shed light predisposition underscore critical functional axis involving miR‐185, VAMP8, DHX9, pathway pathogenesis.
Language: Английский
Citations
0
Clinical and Experimental Pharmacology and Physiology, Journal Year: 2024, Volume and Issue: 51(7)
Published: May 14, 2024
Cervical cancer (CC) is a gynaecological malignancy tumour that seriously threatens women's health. Recent evidence has identified interferon regulatory factor 5 (IRF5), nucleoplasm shuttling protein, pivotal transcription regulating the growth and metastasis of various human tumours. This study aimed to investigate function molecular basis IRF5 in CC development. IRF5, protein phosphatase 6 catalytic subunit (PPP6C) methyltransferase-like 3 (METTL3) mRNA levels were evaluated by quantitative real-time (qRT)-polymerase chain reaction (PCR). PPP6C, METTL3, B-cell lymphoma 2 Bax detected using western blot. Cell proliferation, migration, invasion, angiogenesis apoptosis determined colony formation, 5-ethynyl-2'-deoxyuridine (EdU), transwell, tube formation assay flow cytometry assay, respectively. Glucose uptake lactate production measured commercial kits. Xenograft vivo was used explore role IRF5. After JASPAR predication, binding between PPP6C promoter verified chromatin immunoprecipitation dual-luciferase reporter assays. Moreover, interaction METTL3 methylated RNA (MeRIP). highly expressed tissues cells. silencing significantly inhibited cell glycolytic metabolism cells, while induced apoptosis. Furthermore, absence hindered vivo. At level, might bind with positively regulate expression mRNA. Meanwhile, as downstream target METTL3-mediated m6A modification. modification promote malignant progression which provide promising therapeutic for treatment.
Language: Английский
Citations
3
Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)
Published: Sept. 2, 2024
Language: Английский
Citations
3
Clinica Chimica Acta, Journal Year: 2023, Volume and Issue: 553, P. 117674 - 117674
Published: Nov. 23, 2023
Language: Английский
Citations
8
Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 24(1)
Published: May 2, 2024
Abstract Long non-coding RNAs (lncRNAs) are transcripts that contain more than 200 nucleotides. Despite their inability to code proteins, multiple studies have identified important role in human cancer through different mechanisms. LncRNA lysyl oxidase like 1 antisense RNA (LOXL1-AS1), a newly discovered lncRNA located on chromosome 15q24.1, has recently been shown be involved the occurrence and progression of various malignancies, such as colorectal cancer, gastric hepatocellular carcinoma, prostate non-small cell lung ovarian cervical breast glioma, thymic pancreatic carcinoma. LOXL1-AS1 acts competitive endogenous (ceRNA) via sponging miRNAs, including miR-374b-5p, miR-21, miR-423-5p, miR-589-5p, miR-28-5p, miR-324-3p, miR-708-5p, miR-143-3p, miR-18b-5p, miR-761, miR-525-5p, miR-541-3p, miR-let-7a-5p, miR-3128, miR-3614-5p, miR-377-3p miR-1224-5p promote tumor proliferation, invasion, migration, apoptosis, cycle, epithelial–mesenchymal transformation (EMT). In addition, is regulation P13K/AKT MAPK signaling pathways. This article reviews current understanding biological function clinical significance cancers. These findings suggest may both reliable biomarker potential therapeutic target for
Language: Английский
Citations
2
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: May 1, 2024
Primary testicular lymphoma (PTL) is a rare predominantly occurring in the elderly male population. It characterized by limited response to treatment and heightened tendency towards relapse. Histologically, approximately 90% of PTL cases are classified as diffuse large B-cell lymphomas (DLBCL). Genetic features were delineated scope within several independent studies. Some articles which analyzed genetic characterization DLBCL have incorporated samples, but these been constrained small sample sizes. In addition, there an absence molecular typing studies PTL. This report summarizes common mutational features, copy number variations (CNVs) patients, based on whole-exome sequencing (WES) conducted cohort 25 patients. Among them, HLA, CDKN2A MYD88 had high mutation frequency. we found two core characteristics including genes linked genomic instability (TP53 CDKN2A) immune-related (HLA, MYD88, CD79B). We performed patients into C1 subtype with TP53 mutations C2 HLA mutations. Notably, gene predicted poor outcome most types lymphomas. However, subtype, dominated mutations, better prognosis compared exhibited worse prognosis, aligning our finding that mechanism immune escape was primarily deletions rather than PD-L1/PD-L2 alterations, contrast other DLBCLs. Moreover, calculated tumor burden (TMB) identified TMB can predict recurrence rate Our study underscores significance characteristics, plays crucial role prognostication guiding therapeutic strategies for
Language: Английский
Citations
2