Spectrophotometric and cloud point extraction methods to detect Quercetin Dihydrate in supplement formulations and urine samples DOI Creative Commons
Sadeem Subhi Abed,

Mayasa Mansour Mohammed

Ukrainian Journal of Nephrology and Dialysis, Journal Year: 2024, Volume and Issue: 4(84), P. 75 - 85

Published: Oct. 15, 2024

The accurate detection and quantification of quercetin dihydrate (QRC) are vital for quality control, pharmacokinetic studies, bioavailability assessments in pharmaceutical biological samples. This study aimed to develop validate a cloud point extraction (CPE) method combined with spectrophotometry the sensitive environmentally friendly QRC formulations spiked urine Methods. CPE employed Triton X-114 as non-ionic surfactant extract from process was optimized by evaluating key parameters, including concentration, incubation temperature, time, centrifugation speed. Spectrophotometric analysis conducted before after assess sensitivity linearity method. validated using samples QRC, recovery rates, limits (LOD), evaluated ensure accuracy precision. Results. conditions included an temperature 65°C, 5-minute at 3500 rpm. significantly improved detection, reducing LOD 0.0351 μg/mL (without CPE) 0.0234 (with CPE). exhibited excellent (r² > 0.998) over wide concentration range (1–12 μg/mL). High rates (98.88% 101.6%) low relative standard deviations (RSD < 2%) were observed samples, demonstrating method’s enrichment factor 1.75, preconcentration 4.6. Conclusions. proposed provides simple, sensitive, approach analysis. It offers significant advantages conventional methods, reduced organic solvent use waste generation, making it suitable routine control studies. adaptability complex matrices, such urine, its potential broader applications, other polyphenolic compounds, also demonstrated.

Language: Английский

MiRNAs: main players of cancer drug resistance target ABC transporters DOI
Forogh Mohammadi, Masoumeh Nejatollahi, Farhad Sheikhnia

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Language: Английский

Citations

0
Natural compounds as regulators of miRNAs: exploring a new avenue for treating colorectal cancer DOI
Ahmed S. Doghish,

Sherif S Abdel Mageed,

Osama A. Mohammed

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
Nanoparticle-Based Delivery Systems for Phytochemicals in Cancer Therapy: Molecular Mechanisms, Clinical Evidence, and Emerging Trends DOI
Mahmoud A.H. Mostafa, Hani M. J. Khojah

Drug Development and Industrial Pharmacy, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 31

Published: March 21, 2025

This review examines recent advancements in nanoparticle-based delivery systems for phytochemicals, focusing on their role overcoming multidrug resistance, improving therapeutic efficacy, and facilitating clinical translation. highlights advances nanoparticle-enabled phytochemical to enhance bioavailability, improve outcomes, enable targeted applications. By comparing various nanoparticle systems, formulation methods, efficacy data, it identifies gaps current research guides the development of more effective, next-generation phytochemical-loaded nanocarriers. A systematic literature published between 2000 2024 was conducted using PubMed, Scopus, Web Science. Articles cancer therapy were included. Compounds such as curcumin, resveratrol, quercetin, epigallocatechin gallate demonstrate enhanced anti-cancer when encapsulated nanoparticles, leading improved increased tumor cell targeting, reduced toxicity. Clinical trials indicate regression fewer adverse effects. Emerging approaches-such nanogels, hybrid combination therapies with immune checkpoint inhibitors-further refine treatment efficacy. Nanoparticle-based significantly potential making them promising candidates safer, effective treatments. However, challenges related regulatory guidelines, scalability, long-term safety must be addressed fully realize potential.

Language: Английский

Citations

0
Harnessing Nature's Potential: Exploring Phytoestrogens in Cervical Cancer DOI Creative Commons
Alaa A. A. Aljabali, Rasha M. Bashatwah, Omar Gammoh

et al.

Phytomedicine Plus, Journal Year: 2025, Volume and Issue: unknown, P. 100796 - 100796

Published: April 1, 2025

Language: Английский

Citations

0
A Study of the Impact of Magnesium Supplements on Asthma Control DOI Open Access
Ali Sharifpour, Babak Mahmoudi,

Hossein Saffarfar

et al.

International Journal of Innovative Science and Research Technology (IJISRT), Journal Year: 2024, Volume and Issue: unknown, P. 3136 - 3144

Published: Aug. 21, 2024

Background: In the recent studies, relationship between magnesium and asthma has been more considered; hence, present research aimed to study this issue.  Methodology: The is a Randomized double blind placebo control trial, which conducted on asthmatic patients who have divided into two groups: group one consisting of those taken including supplement. beginning during weeks 4, 8 12, FVC, FEV1, FEV1/FVC ACT score measured. Results: 40 studied in equal groups. improvement rate gradually increased group2 was significantly higher than group1 week 12. It also 4 but not significantly. Moreover, according FVC ratio, cure higher; however, it 8th week. comparison before study. Conclusion: seems that supplement helpful improving clinical spirometric measurements patients.

Language: Английский

Citations

1
Liquid Biopsy and its significance on the Early Detection of Breast Cancer DOI
Farkhonde Hasannejad, Ahmad Fazilat, Keivan Majidzadeh‐A

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 17(3), P. 55 - 81

Published: July 1, 2024

Language: Английский

Citations

0
Unveiling Recurrence Patterns: Analyzing Predictive Risk Factors for Breast Cancer Recurrence after Surgery DOI Creative Commons

M Shahmoradi,

Ahmad Fazilat, Mostafa Ghaderi‐Zefrehei

et al.

Cancer Informatics, Journal Year: 2024, Volume and Issue: 23

Published: Jan. 1, 2024

Breast cancer (BC) stands as the second-leading cause of female-specific cancer-related fatalities globally, necessitating comprehensive research to address its critical aspects. This study aimed explore time intervals between surgery and disease recurrence in BC patients their survival utilizing various parametric semi-parametric models.

Language: Английский

Citations

0
Spectrophotometric and cloud point extraction methods to detect Quercetin Dihydrate in supplement formulations and urine samples DOI Creative Commons
Sadeem Subhi Abed,

Mayasa Mansour Mohammed

Ukrainian Journal of Nephrology and Dialysis, Journal Year: 2024, Volume and Issue: 4(84), P. 75 - 85

Published: Oct. 15, 2024

The accurate detection and quantification of quercetin dihydrate (QRC) are vital for quality control, pharmacokinetic studies, bioavailability assessments in pharmaceutical biological samples. This study aimed to develop validate a cloud point extraction (CPE) method combined with spectrophotometry the sensitive environmentally friendly QRC formulations spiked urine Methods. CPE employed Triton X-114 as non-ionic surfactant extract from process was optimized by evaluating key parameters, including concentration, incubation temperature, time, centrifugation speed. Spectrophotometric analysis conducted before after assess sensitivity linearity method. validated using samples QRC, recovery rates, limits (LOD), evaluated ensure accuracy precision. Results. conditions included an temperature 65°C, 5-minute at 3500 rpm. significantly improved detection, reducing LOD 0.0351 μg/mL (without CPE) 0.0234 (with CPE). exhibited excellent (r² > 0.998) over wide concentration range (1–12 μg/mL). High rates (98.88% 101.6%) low relative standard deviations (RSD < 2%) were observed samples, demonstrating method’s enrichment factor 1.75, preconcentration 4.6. Conclusions. proposed provides simple, sensitive, approach analysis. It offers significant advantages conventional methods, reduced organic solvent use waste generation, making it suitable routine control studies. adaptability complex matrices, such urine, its potential broader applications, other polyphenolic compounds, also demonstrated.

Language: Английский

Citations

0