Circ_0038632 Acts as a Sponge of miR‐4306 to Facilitate Breast Cancer Progression Through Regulating CXCR4 Expression DOI
Yuesheng Zhao, H.Z. Liu, Qi Wang

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(4)

Published: April 1, 2025

ABSTRACT CircRNA plays an important role in the progression of breast cancer. This study focused on molecular mechanism circ_0038632 regulating Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to detect expression levels circ_0038632, microRNA‐4306 (miR‐4306) and C‐X‐C chemokine receptor type 4 (CXCR4) mRNA BC tissues cells. Western blot applied CXCR4 protein metastasis‐related proteins (MMP2 MMP9). Cell proliferation apoptosis were observed by colony formation assay flow cytometry. Scratch Transwell assays performed cell transfer ability. The angiogenesis ability human vascular endothelial cells (HUVECs) measured tube forming assay. Dual luciferase reporting verify relationship between miR‐4306 or CXCR4. In vivo influence tumors. Circ_0038632 highly expressed tissue cells, while lowly expressed. Inhibition would restrain formation, migration, invasion, enhance apoptosis, decrease HUVECs formation. acted as a sponge miR‐4306, inhibitor reverse suppression effect si‐circ_0038632 target overexpression turned growth inhibition caused exogenetic miR‐4306. Importantly, increased via sponging Finally, knockdown inhibited tumor conclusion, contributed malignant miR‐4306/CXCR4 axis.

Language: Английский

Circ_0038632 Acts as a Sponge of miR‐4306 to Facilitate Breast Cancer Progression Through Regulating CXCR4 Expression DOI
Yuesheng Zhao, H.Z. Liu, Qi Wang

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(4)

Published: April 1, 2025

ABSTRACT CircRNA plays an important role in the progression of breast cancer. This study focused on molecular mechanism circ_0038632 regulating Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to detect expression levels circ_0038632, microRNA‐4306 (miR‐4306) and C‐X‐C chemokine receptor type 4 (CXCR4) mRNA BC tissues cells. Western blot applied CXCR4 protein metastasis‐related proteins (MMP2 MMP9). Cell proliferation apoptosis were observed by colony formation assay flow cytometry. Scratch Transwell assays performed cell transfer ability. The angiogenesis ability human vascular endothelial cells (HUVECs) measured tube forming assay. Dual luciferase reporting verify relationship between miR‐4306 or CXCR4. In vivo influence tumors. Circ_0038632 highly expressed tissue cells, while lowly expressed. Inhibition would restrain formation, migration, invasion, enhance apoptosis, decrease HUVECs formation. acted as a sponge miR‐4306, inhibitor reverse suppression effect si‐circ_0038632 target overexpression turned growth inhibition caused exogenetic miR‐4306. Importantly, increased via sponging Finally, knockdown inhibited tumor conclusion, contributed malignant miR‐4306/CXCR4 axis.

Language: Английский

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