The Association between Caffeine Intake and the Colonic Mucosa-Associated Gut Microbiota in Humans—A Preliminary Investigation

Nutrients, Journal Year: 2023, Volume and Issue: 15(7), P. 1747 - 1747

Published: April 3, 2023

We examined the association between caffeine and coffee intake community composition structure of colonic microbiota. A total 34 polyp-free adults donated 97 biopsies. Microbial DNA was sequenced for 16S rRNA gene V4 region. The amplicon sequence variant assigned using DADA2 SILVA. Food consumption ascertained a food frequency questionnaire. compared relative abundance taxonomies by low (<82.9 mg) vs. high (≥82.9 never or <2 cups 2 ≥3 intake. False discovery rate-adjusted p values (q values) <0.05 indicated statistical significance. Multivariable negative binomial regression models were used to estimate incidence rate ratio its 95% confidence interval having non-zero count certain bacteria level. Higher related higher alpha diversity (Shannon index < 0.001), Faecalibacterium Alistipes, lower Erysipelatoclostridium 0.05). After adjustment vitamin B2 in multivariate analysis, significant inverse remained statistically significant. Our preliminary study could not evaluate other prebiotics coffee.

Language: Английский

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Multiomics provides insights into the succession of microbiota and metabolite during plant leaf fermentation

Environmental Research, Journal Year: 2023, Volume and Issue: 221, P. 115304 - 115304

Published: Jan. 14, 2023

Language: Английский

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Early fecal microbiota transplantation from high abdominal fat chickens affects recipient cecal microbiome and metabolism

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Abdominal fat deposition (AFD) in chickens is closely related to the gut microecological balance. In this study, microbiota from high-AFD was transplanted into same strain of 0-day-old chicks via fecal transplantation (FMT). The FTM with high AFD had no obvious effects on growth traits, adult body weight, carcass abdominal and percentage, but did reduce coefficient variation traits. FMT significantly decreased cecal microbiome richness, changed structure, regulated biological functions associated energy metabolism synthesis. Additionally, metabolite composition metabolic function recipient were also altered those controls. Transplantation chicken promoted fatty acid elongation biosynthesis reduced vitamins, steroids, carbohydrates cecum. These findings provide insights mechanisms by which affect host profiles deposition.

Language: Английский

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Are there consistent effects of gut microbiota composition on performance, productivity and condition in poultry?

Poultry Science, Journal Year: 2024, Volume and Issue: 103(6), P. 103752 - 103752

Published: April 10, 2024

Microbiome of the gastrointestinal tract (GIT) has been identified as one crucial factors influencing health and condition domestic animals. The global poultry industry faces challenge understanding complex relationship between gut microbiota composition performance-related traits in birds. Considerable variation exists results correlational studies using either 16S rRNA profiling or metagenomics to identify bacterial taxa associated with performance, productivity, (e.g., body weight, growth rate, feeding efficiency, egg yield). In this review, we survey existing reports, discuss research approaches, consistently linked improved deteriorated performance across individual poultry-focused studies. Our revealed high methodological heterogeneity, which was contrast vastly uniform focus mainly on chicken (Gallus gallus) a model. We also show that most frequently used manipulative experiments commercial probiotics intended for use species Lactobacillus, Bacillus, Enterococcus, Bifidobacterium) do not overlap bacteria correlated their (Candidatus Arthromitus, Methanobrevibacter). conclusions urge increased standardization veterinary field. highlight need bridge gap experimental applications animal science. To better understand causality observed relationships, future should involve broader range host includes both agricultural wild models, well age groups.

Language: Английский

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Effects of different energy levels in low-protein diet on liver lipid metabolism in the late-phase laying hens through the gut-liver axis

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 11, 2024

Abstract Background The energy/protein imbalance in a low-protein diet induces lipid metabolism disorders late-phase laying hens. Reducing energy levels the to adjust energy-to-protein ratio may improve fat deposition, but this also decreases performance of This study investigated mechanism by which different influences liver hens through enterohepatic axis guide feed optimization and nutrition strategies. A total 288 were randomly allocated normal-energy normal-protein group (positive control: CK) or 1 3 groups: low-energy (LL), (NL), high-energy (HL) groups. ratios CK, LL, NL, HL diets 0.67, 0.74, 0.77, 0.80, respectively. Results Compared with CK group, egg quality deteriorated increasing intake fed diet. Hens had significantly higher triglyceride, cholesterol, acetyl-CoA carboxylase, fatty acid synthase levels, lower hepatic lipase compared group. Liver transcriptome sequencing revealed that genes involved beta-oxidation ( ACOX1 , HADHA EHHADH ACAA1 ) downregulated, whereas related synthesis SCD FASN ACACA upregulated LL Comparison cecal microbiome showed an diet, Lactobacillus Desulfovibrio enriched, riboflavin was suppressed. Cecal metabolites most affected included several vitamins, such as (vitamin B 2 ), pantethine 5 derivative), pyridoxine 6 4-pyridoxic acid. Conclusion disorder due deficiencies vitamin originating from be underlying reason for accumulation Based on present study, we propose targeting derivative) might effective strategy improving

Language: Английский

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16S rDNA sequencing combined with metabolomic probes to investigate the effects of Salmonella Pullorum on gut microbes and metabolites in broilers

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Pullorum disease (PD) caused by Salmonella (SP) results in high mortality chicks and potential carriers adult chickens, negatively affecting growth egg production. This study identified SP infection 100-day-old White Plymouth Rock hens serum plate agglutination fecal anal swab polymerase chain reaction. SP-infected broilers were classified into positive (P) negative (N) groups using hematoxylin-and-eosin staining, metabolome sequencing, 16S rDNA to investigate the effects of on metabolites microorganisms cecum broilers. Groups had different degrees inflammatory cell infiltration cecum, spleen, liver, lung tissues. The diversity bacterial flora P N differed significantly (P < 0.05). o__Lactobacillales o__Verrucomicrobiota higher Group than At genus level, g__Akkermansia was Metabolome sequencing contents screened 77 differential at secondary metabolite level. 11 metabolites, including 2,4-dimethylbenzaldehyde, 3a,6b,7b,12a-tetrahydroxy-5b-cholanoic acid, LysoPG 19:1, differentially expressed A combined analysis cecal content metabolomics 28 genera associated with 38 Specific such as Corynebacterium Roseobacter have particularly prominent metabolites. These findings highlight significant alterations gut microbial composition metabolic functions due infection. taxa this may provide insights underlying mechanisms PD pathogenesis biomarkers for management.

Language: Английский

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Microbiome-metabolomics analysis insight into the effects of dietary chromium picolinate on abdominal fat deposition and lipid metabolism of ducks

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105040 - 105040

Published: March 1, 2025

This research endeavored to explore the impact of Cr-Pic on abdominal fat deposition and lipid metabolism ducks, as well uncover mechanisms at play identify optimal dietary supplementation level Cr-Pic. The results showed that adding 0.6 mg/kg observably increased body weight serum levels adiponectin leptin, decreased total cholesterol, triglyceride, low-density lipoprotein cholesterol in ducks 14 days (P < 0.05). addition % was discovered regulate meat quality through its effects reducing drip loss, downregulating mRNA expression genes involved synthesis liver, upregulating associated with degradation transport these tissues Untargeted metabolic profiling indicated distinct metabolites between Control groups were predominantly pathways involving amino acids. Microbiome analysis administration altered microbial genera related adjustment cecum such increasing proportion Bacteroides Lactobacillus, decreasing proportions Alistipes, Methanobrevibacter, Romboutsia, Peptostreptococcus In summary, shows promise accumulation by regulating intestinal composition acids are linked metabolism.

Language: Английский

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Gut bacteria and host metabolism: The keys to sea cucumber (Apostichopus japonicus) quality traits

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144178 - 144178

Published: April 1, 2025

Language: Английский

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The flight of chicken genomics and allied omics-a mini review

Ecological Genetics and Genomics, Journal Year: 2023, Volume and Issue: 29, P. 100201 - 100201

Published: Sept. 25, 2023

Language: Английский

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Assessing the impact of three feeding stages on rumen bacterial community and physiological characteristics of Japanese Black cattle

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 28, 2024

Abstract In Japan, Japanese Black cattle, known for their exceptional meat quality owing to abundant intramuscular fat, undergo a unique three-stage feeding system with varying concentrate ratios. There is limited research on physiological and rumen microbial changes in cattle during these stages. Therefore, this study aimed examine steers three stages: early (T1, 12–14 months), middle (T2, 15–22 late (T3, 23–30 months). The bacteria of 21 per phase was analyzed using 16S rRNA gene sequencing. Rumen bacterial diversity significantly higher T1, distinct distribution, than T2 T3. Specific phyla genera were exclusive each stage, reflecting the shifts feed composition. Certain dominated stage: T1 had Flexilinea , Streptococcus Butyrivibrio Selenomonas Kandleria ; Bifidobacterium Shuttleworthia Sharpea T3 Acetitomaculum Mycoplasma Atopobium Howardella . Correlation analysis revealed significant associations between certain populations parameters. These findings indicate that energy content composition are associated ruminal alterations. This may guide strategies improve health productivity by modifying diets specific fattening

Language: Английский

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