The therapeutic efficacy of neem leaf extract against coinfection with Chlamydophila psittaci and low pathogenic avian influenza virus H9N2 in broiler chickens DOI Creative Commons

Ahmed M. Hegazy,

Amira Morsy,

Heba M. Salem

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(10), P. 104089 - 104089

Published: July 10, 2024

Avian chlamydiosis is a serious avian infection that carries significant zoonotic danger to the poultry industry. The respiratory co-infections caused by low pathogenic influenza virus H9N2 (LPAIV H9N2) also cause financial losses in purpose of this study was examine pathogenicity Chlamydophila psittaci, and LPAIV individually combination broiler chickens, as well determine whether or not aqueous neem (Azadirachta indica) leaf extract effective against infections these pathogens. Therefore, 120 cobb chicks were equally divided into 4 groups (30 birds each) with triplicates 10 birds. Broilers group 1 (G1) infected only C. broilers 2 (G2) H9N2, 3 (G3) psittaci (G4) remained challenged non-treated any therapeutic preventive treatment (negative control). At 21 d postinfection (dpi), G1, G2, G3 subgroups each: subgroup (A) untreated (positive control), (B) received oxytetracycline for 5 consecutive d, (C) 8% d. multiplication various tissues evaluated using Giemsa staining data showed much higher lung, spleen, liver from 6 h dpi, but heart 8 dpi. During simultaneous co-infection G3, developed clinical symptoms postmortem lesions (PM). Quantitative real-time polymerase chain reaction (qRT-PCR) used detect viral shedding oropharyngeal cloacal swabs between dpi cycle threshold (CT) values ranging 22 24. In contrast, bacterial began after continued until CT 23 26. Administration an at concentration (Group C) resulted numerical rise average body weight across all third fourth week, reduction replication gut treated compared those B). Overall, pose considerable risk business, which big threat. To control oral supplementation recommended. This improves birds' performance, evidenced increase their weight. addition, application lowers within diminishes shedding.

Language: Английский

Spirulina (Arthrospira platensis) Used as Functional Feed Supplement or Alternative Protein Source: A Review of the Effects of Different Dietary Inclusion Levels on Production Performance, Health Status, and Meat Quality of Broiler Chickens DOI Creative Commons
Antonia Lestingi, Mahmoud Alagawany, Alessandro Di Cerbo

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1537 - 1537

Published: Nov. 23, 2024

The broiler industry is pivotal in meeting the growing global demand for highly nutritious animal protein foods. Hence, there a continuous interest identifying novel, alternative, and even unconventional feed resources that could help sustainably support chicken meat production quality. In this view, microalga Spirulina (Arthrospira, formerly Spirulina, platensis), due to its unique chemical composition some ecological advantages offered by cultivation over traditional agriculture, has attracted great attention poultry sector potential application diets, either as functional supplement or replacer of conventional sources such soybean meal. studies conducted so far seem have confirmed many initial expectations regarding may derive from dietary supplementation, documenting capacity positively influence intestinal general health status chickens, leading improved preserved productive performance (under normal challenging conditions, respectively), well increased disease resistance survivability. Furthermore, supplementation been shown induce positive changes important traits However, at present, inclusion diet, especially but not solely relation use an alternative source, presents several technical economic limitations. To increase overall awareness around actual usefulness practical usability novel natural component review paper seeks provide comprehensive integrated presentation what currently known about topic, highlighting critical issues are still pending would require further research efforts.

Language: Английский

Citations

5
SARS-CoV-2 Mpro and its inhibitory peptides from Spirulina platensis: Preparation, identification, in vitro and in silico characterization of activity and binding DOI
Qiaohui Zeng,

Zhi-En Yao,

Xianmin Zhou

et al.

Algal Research, Journal Year: 2025, Volume and Issue: 86, P. 103955 - 103955

Published: Feb. 8, 2025

Language: Английский

Citations

0
Screening and mechanism study on SARS-CoV-2 Mpro inhibitory of peptides from Spirulina platensis DOI Creative Commons
Qiaohui Zeng,

Zhi-En Yao,

Xianmin Zhou

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 20, 2024

Abstract In this study, four kinds of Spirulina platensis ( S. platensis) peptides were obtained by enzymolysis protein after different pretreatment to S. platensis powders. The novel coronavirus main protease (SARS-CoV-2 protease, Mpro) was successfully prepared heterologous expression in E. coli and its activity detected. After identification sequences, highly active synthesized their inhibition effects on Mpro verified. Subsequnently, the mechanism action between them analyzed computer simulations. Results showed that extraction rate from was 96.11%, 845.90 U mg-1, peptide MQGPNY could inhibit with 20.21%±3.8% at concentration 2 mM. Fuerthermore, results Simulation forms pairs hydrogen bonds Mpro, which are Gln189, Ser46, Thr26 Glu166. addition, there nine free residues involved hydrophobic contacts, His164, His41, Thr24, Met49, Thr45, Thr25, Gly143, Asn142, Cys145 Met165. This study developed natural platensis, inhibited Mpro. Besides, MQGPN acting revealed method Simulation. foundings provide theoretical support for use hydrolysates functional food supplement formulations post-epidemic era.

Language: Английский

Citations

0
The therapeutic efficacy of neem leaf extract against coinfection with Chlamydophila psittaci and low pathogenic avian influenza virus H9N2 in broiler chickens DOI Creative Commons

Ahmed M. Hegazy,

Amira Morsy,

Heba M. Salem

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(10), P. 104089 - 104089

Published: July 10, 2024

Avian chlamydiosis is a serious avian infection that carries significant zoonotic danger to the poultry industry. The respiratory co-infections caused by low pathogenic influenza virus H9N2 (LPAIV H9N2) also cause financial losses in purpose of this study was examine pathogenicity Chlamydophila psittaci, and LPAIV individually combination broiler chickens, as well determine whether or not aqueous neem (Azadirachta indica) leaf extract effective against infections these pathogens. Therefore, 120 cobb chicks were equally divided into 4 groups (30 birds each) with triplicates 10 birds. Broilers group 1 (G1) infected only C. broilers 2 (G2) H9N2, 3 (G3) psittaci (G4) remained challenged non-treated any therapeutic preventive treatment (negative control). At 21 d postinfection (dpi), G1, G2, G3 subgroups each: subgroup (A) untreated (positive control), (B) received oxytetracycline for 5 consecutive d, (C) 8% d. multiplication various tissues evaluated using Giemsa staining data showed much higher lung, spleen, liver from 6 h dpi, but heart 8 dpi. During simultaneous co-infection G3, developed clinical symptoms postmortem lesions (PM). Quantitative real-time polymerase chain reaction (qRT-PCR) used detect viral shedding oropharyngeal cloacal swabs between dpi cycle threshold (CT) values ranging 22 24. In contrast, bacterial began after continued until CT 23 26. Administration an at concentration (Group C) resulted numerical rise average body weight across all third fourth week, reduction replication gut treated compared those B). Overall, pose considerable risk business, which big threat. To control oral supplementation recommended. This improves birds' performance, evidenced increase their weight. addition, application lowers within diminishes shedding.

Language: Английский

Citations

0