Immunization with OPN5 increased seasonal degradation of reproductive activity in Magang ganders

Poultry Science, Journal Year: 2025, Volume and Issue: 104(2), P. 104753 - 104753

Published: Jan. 1, 2025

To investigate the regulatory mechanism mediated by hypothalamic OPN5 on seasonal changes in reproductive activities of domestic geese, 60 Magang ganders their breeding period were selected for experiment and evenly divided into an immunization group(OPN5-IM) a control group. On days 0, 15 30, immunized group with OPN5-KLH protein vaccine, same amount blank emulsified vaccine. Additionally, 120 female geese provided to stimulate male geese. The results showed that arrangement spermatogenic cells was disturbed, number sperm decreased, testicular weight, seminiferous tubule area, length diameter, epithelium thickness decreased significantly natural day prolonged. Moreover, concentration testosterone LH while PRL increased. prolonged photoperiod affected gene expression GnRH-I, VIP, FSHβ, FSHR, LHβ, PRL, PRLR ganders. Specifically, LHβ hypothalamus pituitary Following immunization, anti-OPN5 antibody titer OPN5-IM notably higher than degeneration severe compared control, as evidenced significant reduction 60. concentrations lower group, whereas higher. PRLR. mRNA TRH, DIO2, TSHR expressions lower. However, DIO3 up-regulated In conclusion, our indicated performance degraded from non-breeding daylight extended. Immunization against increased down-regulated TSH-DIO2/DIO3 pathway, further affect HPG axis accelerate degradation activity. Therefore, may play important mediating role light-regulating

Language: Английский

Integrated Transcriptomic–Metabolomic Analysis Reveals the Effect of Different Light Intensities on Ovarian Development in Chickens

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8704 - 8704

Published: Aug. 9, 2024

Light is a key environmental factor regulating reproduction in avians. However, the mechanism of light intensity ovarian development still unclear. In this study, 5-week-old (5 wk) partridge broiler breeders were randomly divided into low-light-intensity group (LL group) and natural-light-intensity (NL (

Language: Английский

Effects of dietary supplementation by modified palygorskite and essential oil/palygorskite complex on growth performance and intestinal flora composition of broilers with diarrhoea

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104379 - 104379

Published: Oct. 9, 2024

With the development trend of industry, it can be seen that substitution antibiotics and reduction zinc oxiden is still hot spot industry. Diarrhea inflammation occur frequently during livestock poultry production, which difficult to control. This experiment aimed explore effects mechanisms dietary supplementation modified palygorskite (Mpal) essential oil/ composite (EO-PGS) on disease resistance intestinal inflammatory damage in diarrhea broiler. In this experiment, there were a total 420 broilers 10-day-old selected divided into 7 groups (n = 60), nondiarrhea group fed with basal diet (normal control, NC), (diarrhea DC), rest test (diarrhea), supplemented 1 kg/t, 2 kg/t 4 oils/ complex (EO-PGS 1kg/T, EO-PGS 2kg/T, 4kg/T) diet, respectively, (Mpal Mpal diets, respectively. The lasted for 8 d. results showed compared normal broilers, index remained around 2.0 persistent mild period. duodenal epithelial cells damaged shed, goblet increased, infiltrated, diffuse congestion hemorrhage lamina propria, serum lipopolysaccharides (LPS) content, malondialdehyde (MDA) content increased significantly (P < 0.05). superoxide dismutase (SOD) activity immunoglobulin-M (IgM) levels decreased, while immunoglobulin-G (IgG) complement 3 (C3) expression cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), nuclear factor κB (NF-κB) was upregulated d 5 abundance Bacteroides duodenum Proteobacteria Feeding diets EO-PSG average weight 0.05), reduced index, improved immunity by increasing IgG, IgM, C3 (C4) enhanced antioxidant enzyme glutathione peroxidase (GSH-PX) SOD activity, MDA LPS levels, decreased proinflammatory factors cell alleviated injury, hemorrhage, infiltration injury from 8. Meanwhile, regulated microbiota, Bacteroidetes at phylum level Microbial richness diversity microbiota feeding EO-PGS. beta flora EO-PGS, microbial community composition could relatively easily distinguished NC DC groups. As result LEfSe analysis, f_Clostridiaceae g_Coprococcus enriched caecum o_Bacteroidales, f_Rikenllaceae g_Peptococcus between (P<0.05). conclusion, improve alleviate but effect not significant. It recommended or added broilers' according degree diarrhea, continuous more than

Language: Английский