Comparative analysis of amino acids and volatile compounds as markers in Lueyang black-boned chicken and Arbor Acres broilers

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 104897 - 104897

Published: Feb. 1, 2025

Derived from a long history of artificial and natural selection, Lueyang black-boned chicken is preferred by consumers due to its unique meat characteristics. The primary aim this study was systematically identify key amino acids volatile organic compounds (VOCs) that differentiate (LBC) AA chicken. contents essential acids, non flavor were higher in the breast muscle LBC compared Volatilomic analysis revealed contains various VOCs, including esters, aldehydes, ketones, alcohols, alkanes furans. Random forest showed major VOCs esters while leg alcohols. Additionally, partial least squares discriminant identified Carbonic acid, monoamide, N-(2-pentyl)-N-octyl-, ethyl ester, 1-Octen-3-ol, 1-Undecanol as muscle, with 1-Hexanol 1-Dodecanol potential discriminatory markers LBC. Our findings provide comprehensive profile meat, which could serve basis for breeding chickens improved quality.

Language: Английский

---

Transcriptome analysis of multiple tissues and identification of tissue-specific genes in Lueyang black-bone chicken

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104986 - 104986

Published: March 4, 2025

Systematically constructing a gene expression atlas of poultry tissues is critically important for advancing research and production. In this study, the profiles 9 major Lueyang black-bone chicken were successfully constructed by transcriptome sequencing technology. Through in-depth analysis data, total 10 housekeeping genes (HKGs) 87 marker (MGs) identified. Furthermore, applying weighted co-expression network (WGCNA), we delineated nine tissue-specific modules 90 hub genes, offering novel insights into regulatory networks underlying expression. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment showed that HKGs predominantly involved in maintaining fundamental cellular functions, with significant pathways related to oxidative phosphorylation, cell cycle regulation, DNA replication. MGs closely associated physiological providing valuable molecular mechanisms governing tissue functionality. Notably, through multidimensional validation, EEF1A1 FTH1 confirmed exhibit cross-tissue stability, establishing them as ideal reference multi-tissue qPCR experiments chickens. Additionally, identified including TNNT2, PIT54, SFTPC, PGM1, which are specific heart, liver, lung, breast muscle, respectively. The results study have scientific value expanding selection elucidating mechanisms, provide solid theoretical support technical guidance breeding improvement production practice optimization.

Language: Английский

---

Developmental changes of skin quality from breast, back, and thigh of Pekin ducks from 1 to 6 weeks of age

Poultry Science, Journal Year: 2025, Volume and Issue: 104(2), P. 104759 - 104759

Published: Jan. 6, 2025

Good skin quality not only improved carcass but also increased consumer demand for fresh poultry meat. This study aimed to investigate the developmental changes in growth and of Pekin ducks during 1-6 weeks age. The samples were collected from breast, back, thigh tissues six male at end each week. strength, thickness, collagen content as well related gene expressions determined evaluation quality. results showed that body weight, absolute areas, density, epidermal dermal thickness (breast thigh), shearing force, piercing force (back linearly quadratically with age, reaching a plateau 5-6 age (P < 0.05). mRNA IGF-1 FGFR1 cell proliferation highest 3 while expression FGF14 EGF associated synthesis reached maximum values 5 Additionally, IGF1R FGFR2 upregulated breast skins 1 week old back compared birds other In conclusion, pattern structure linear manner was quadratic manner, which target genes synthesis.

Language: Английский

---

Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens

Poultry Science, Journal Year: 2024, Volume and Issue: 104(1), P. 104210 - 104210

Published: Aug. 13, 2024

Dermal hyperpigmentation stands out among the various skin pigmentation phenotypes in chickens, where most other variants affect feather color and patterning predominantly. Despite numerous black chicken breeds worldwide, only a select few exhibit comprehensive pigmentation, which encompasses skin, meat, flesh, bones. The process of melanin is intricate develops successively. Historically, research has concentrated primarily on specific developmental points or stages, but fewer studies have examined entire transcriptome across timeline development embryo integument. In our investigation, we undertook sequencing samples from day 4 to 13 incubation. Our results showed that melanoblasts continued migrate E4 epidermis until E12. Beginning with E6, was synthesized transferred epidermal cells follicles large quantities, genes such as DCT, TYR, TYRP1, MITF played key role this process, significantly different white-skinned chickens. There were 854 differentially expressed between E7 E8. At stage, melanocytes formed dendritic forms keratinocytes, while dorsal became visibly dark. addition, CDH3 , core factor involved variety biological processes, may an important impact pigmentation. Collectively, findings unveiled phased relationship canonical pathway non-canonical E13. These analyses illuminated gene regulatory mechanism provided foundational data pertained

Language: Английский

---