Bile acid disorders and intestinal barrier dysfunction are involved in the development of fatty liver in laying hens DOI Creative Commons
Lihua Zhao, Qiuyu Jiang, Jiaqi Lei

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104422 - 104422

Published: Oct. 13, 2024

The pathogenesis of fatty liver is highly intricate. role the gut-liver axis in development has gained increasing recognition recent years. This study was conducted to explore bile acid signaling and gut barrier liver. A total 100 "Jing Tint 6" laying hens, 56-week-old, were used fed basal diets until 60 weeks age. At end experiment, thirty individuals selected based on degree hepatic steatosis. hens with minimal steatosis (< 5 %) chosen as healthy controls, while those severe (> 33 classified group. Laying controls showed significant differences body weight, index, abdominal fat ratio, feed conversion ratio (FCR), albumin height, Haugh unit, biochemical indexes. results metabolomics revealed a clear separation profiles between group multiple secondary acids decreased group, indicating disordered metabolism. Additionally, mRNA levels farnesoid X receptor (FXR) genes related transport significantly both terminal ileum Moreover, exhibited decreases ileal crypt depth, number goblet cells, expression tight junction-related proteins, alongside increase permeability. Collectively, these findings suggest that acids, suppressed FXR-mediated signaling, impaired intestinal function are potential factors promoting These insights indicate regulating enhancing may become new preventive therapeutic strategies for near future.

Language: Английский

Chronic Cr(VI) exposure-induced biotoxicity involved in liver microbiota-gut axis disruption in Phoxinus lagowskii Dybowski based on multi-omics technologies DOI

Xinchi Shang,

Xinghua Che,

Kai Ma

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125759 - 125759

Published: Jan. 1, 2025

Language: Английский

Citations

1
Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis) DOI Creative Commons
Liqin Ji,

Yisen Shangguan,

Qing Shi

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 544 - 544

Published: Feb. 13, 2025

To assess the impact of tannic acid (TA) on hepatic health Chinese soft-shelled turtle, individuals were fed diets containing 0 (CG), 0.5, 1, 2, and 4 g/kg TA for 60 days (0 hps). Afterwards, turtles challenged with 15 °C cold stress 24 h (24 hps) then recovered to 28 (48 The results indicated that has a significant toxic effect after 60-day administration. T-SOD, CAT, GSH-Px, T-AOC activities in TA2 increased at hps (p < 0.05) compared CG. Similarly, Sod1, Sod2, Cat, Gsh-px3, Gsh-px4 mRNA levels higher than other four groups 0.05). histology data showed induced injuries liver temperature fluctuation, whereas 2 effectively attenuated injuries. A total 202 differentially expressed metabolites (DEMs) CG vs. 115 DEMs LTCG LTTA2 separately detected by metabolome. cephalosporin C, protoporphyrin, bis(2-ethylhexyl) phthalate, 2-acetamidoethylphosphonat, verbasosid, norcocain, obvious TA2. Furthermore, valienone 7-phosphate, 5-HETE, pregnanetriolone, 4-keto-anhydrotetracyclin, dephospho-CoA, lysoPC(18:4(6Z,9Z,12Z,15Z)/0:0) top LTTA2. "adipocytokine signaling pathway" "AMPK key pathways enriched TA2, while "fatty biosynthesis", "steroid "linoleic metabolism" most abundant Generally, this research could protect from fluctuations improving antioxidant capacity, reducing histological injuries, regulating lipid-related pathways.

Language: Английский

Citations

0
Multi-omics Reveal the Effects and Regulatory Mechanism of Dietary Echinocystic Acid Supplementation on Abdominal Fat and Liver Steatosis in Broiler Chickens DOI Creative Commons

Lianggui Xiao,

Jia-Huan Liu,

Liangshan Qin

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104981 - 104981

Published: March 5, 2025

The accumulation of abdominal fat and the metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent problems in poultry industry, seriously compromise broiler health reduce economic benefits. Echinocystic acid (EA), a natural product with anti-inflammatory antioxidant effects, has been demonstrated to deposition improve intestinal inflammation mice. However, it not reported research. In this study, we employed chicken hepatocytes (Leghorn male hepatoma cells, LMHs) construct an oleic palmitic (OA/PA)-induced MAFLD model vitro 60 K90 chickens were induced by high-fat diet (HFD) examine impact EA on liver-lipid metabolism deposition. Moreover, metabolomic analysis, 16S rDNA gene sequencing, transcriptomic profiling performed determine mechanism EA. results showed that (10 μM) significantly reduced triglyceride (TG) total cholesterol (TC) levels vitro. without affecting growth performance. decreased TC, TG, low-density lipoprotein-cholesterol (LDL-C) levels, increased high-density (HDL-C) blood. Additionally, supplementation altered composition microbiota, particularly decreasing ratio Firmicutes Bacteroidetes. Furthermore, metabolomics analysis revealed abundance metabolites related arginine mitochondrial oxidation pathways, these predicted be positively correlated gut genera enriched also expression patterns genes lipid inflammation, CYP7A1, CYP7B1, CYP3A5, ACAT, which PPAR signaling pathway steroid hormone metabolism. correlation there was close between differential metabolites, profiles. Collectively, indicated may alleviate regulating modulating microbiota. candidate feed additive prevent industry.

Language: Английский

Citations

0
Manganese deficiency or dietary manganese(III) oxide nanoparticle supplementation: consequences for hematology, and intestinal and brain immunity in rats DOI Creative Commons

Karolina Różaniecka-Zwolińska,

Ewelina Cholewińska, Bartosz Fotschki

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 8, 2025

The study aimed to determine the effect of manganese (Mn) exclusion from mineral mixture added rat diet and replacing recommended level MnCO3 (65 mg Mn/kg diet) with Mn2O3 nanoparticles (Mn2O3NPs) in on blood hematology selected immunological indices blood, jejunum, brain. experiment was conducted twenty-four, Wistar rats divided into 3 equal groups. control (K) group received a containing 65 mg/kg additional Mn originating mixture), B (negative control) fed deprived mixture, N Mn2O3NPs preparation. All experimental diets for 12 weeks. At end experiment, samples brain were collected all each group. led anemia, worsened body's immune response, caused systemic local inflammation as indicated e.g. by decreased RBC, HCT, HGB, CRP IgA IgG well an increased IL-2, TNF-α IL-6 jejunum. In turn, response increase Cp activity, levels IgG. Analogical changes not observed jejunum or level. obtained results may suggest that body has activated adaptive mechanisms efficiently limit spread system disorders throughout body.

Language: Английский

Citations

0
Impact of bacteroides uniformis on fatty liver hemorrhagic syndrome in dawu golden phoenix laying hens: modulation of gut microbiota and arachidonic acid metabolism DOI Creative Commons
Yu Zhang, Rong Ma, Xuqin Du

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

This study explored the impact of Bacteroides uniformis (B. uniformis) on fatty liver hemorrhagic syndrome (FLHS) induced by a high-energy and low-protein (HELP) diet in laying hens, mainly focusing hepatic lipid metabolism, gut microbiota, arachidonic acid (AA) metabolism. A total 120 Dawu Golden Phoenix hens (210-day-old) were randomly divided into four groups. The control group (CON) was fed standard received daily gavage PBS, while other groups with HELP to induce FLHS PBS (MOD), 1 × 109 CFU/ml B. (BUL), 1011 (BUH) for 70 days. All administered ml gavage. Each had 6 replications 5 per replication. results showed that increased egg production rate feed conversion ratio decreased body weight, index, abdominal fat (p < 0.05). treatment reduced accumulation reducing levels Triglyceride (TG), Total cholesterol (TC), low-density lipoprotein (LDL-C), alanine transaminases (ALT), aspartate (AST) serum significantly elevated high-density (HDL-C) indicated altered microbiota. Specifically, abundance higher, relative abundances Treponema, Helicobacter, Spirochaetota lower than those MOD Moreover, targeted metabolomic analysis supplementation 6-keto-PGF1α AA levels, along thromboxane B2 (TXB2), leukotriene D4 (LTD4), 8-isoprostaglandin F2α (8-iso-PGF2α), 12S-hydroxyeicosatetraenoic (12S-HETE), 15S-hydroxyeicosatetraenoic (15S-HETE), 9-S-hydroxy-octadecadienoic (9S-HODE), 13-S-hydroxy-octadecadienoic (13S-HODE) In conclusion, oral intake can improve function, thereby helping ameliorate hens.

Language: Английский

Citations

0
Bile acid disorders and intestinal barrier dysfunction are involved in the development of fatty liver in laying hens DOI Creative Commons
Lihua Zhao, Qiuyu Jiang, Jiaqi Lei

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104422 - 104422

Published: Oct. 13, 2024

The pathogenesis of fatty liver is highly intricate. role the gut-liver axis in development has gained increasing recognition recent years. This study was conducted to explore bile acid signaling and gut barrier liver. A total 100 "Jing Tint 6" laying hens, 56-week-old, were used fed basal diets until 60 weeks age. At end experiment, thirty individuals selected based on degree hepatic steatosis. hens with minimal steatosis (< 5 %) chosen as healthy controls, while those severe (> 33 classified group. Laying controls showed significant differences body weight, index, abdominal fat ratio, feed conversion ratio (FCR), albumin height, Haugh unit, biochemical indexes. results metabolomics revealed a clear separation profiles between group multiple secondary acids decreased group, indicating disordered metabolism. Additionally, mRNA levels farnesoid X receptor (FXR) genes related transport significantly both terminal ileum Moreover, exhibited decreases ileal crypt depth, number goblet cells, expression tight junction-related proteins, alongside increase permeability. Collectively, these findings suggest that acids, suppressed FXR-mediated signaling, impaired intestinal function are potential factors promoting These insights indicate regulating enhancing may become new preventive therapeutic strategies for near future.

Language: Английский

Citations

0