Proteomics signatures associated with hip arthropathy in ankylosing spondylitis

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: May 14, 2025

Objective Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton and peripheral joints, with hip arthropathy representing severe complication that critically impairs mobility. While persistent inflammation hallmark of AS, molecular mechanisms driving involvement remain poorly characterized. This study aimed to identify validate protein biomarkers associated progression in AS through integrated proteomic functional analyses. Methods Liquid chromatography-mass spectrometry (LC–MS/MS) was employed screen for differentially abundant proteins (DAPs) joint tissues from 30 patients 14 non-AS controls. Bioinformatics methods were utilized key DAPs. Results A total 2,050 relatively quantified, 109 DAPs (34 upregulated 75 downregulated) meeting criteria p < 0.05 fold change ≥1.5 or ≤0.67. Enriched GO terms represented by included Wnt signaling pathway, MAPK cascade, antigen processing presentation exogenous peptide via MHC class I. The main Kyoto Encyclopedia Genes Genomes (KEGG) pathways PI3K-Akt ribosome, metabolic pathways, neutrophil extracellular trap formation. protein–protein interaction (PPI) network identified ribosomal (RPs), including RPS11, RPS24, RPL35, RPS3A, RPS6, RPS8, RPS14, RPS7, as highly connected hub proteins. These RPs significantly enriched pathogenesis, particularly osteoblast differentiation T cell-mediated immune regulation. Conclusion Based on proteomics approaches bioinformatics analysis, this discovered AS. It may provide potential screening tools therapeutic targets warranting further research validation.

Language: Английский

Transcriptomic analysis of bone transport reveals different functions between both ends

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: May 23, 2025

Background Bone fractures are common in both young and elderly populations, bone transport surgery is a critical orthopedic procedure for patients with severe fractures, defects, non-unions. However, the specific molecular mechanisms driving healing during transport, particularly roles of compressive tensile ends, remain poorly understood. Methods We utilized transcriptomic analysis tissues from rat model to explore differential gene expression patterns associated ends. Results 233 differentially expressed genes (DEGs) were identified end (TE) group 317 DEGs (CE) group, compared control group. These enriched distinct biological processes. The TE was primarily processes such as ossification, extracellular matrix organization, development. Key including Bglap, Acan, Mmp13, Runx2, upregulated, highlighting their osteogenesis. In contrast, CE showed enrichment related myogenesis, muscle system skeletal tissue Core Chrna1, Chrnd, Myod1, Rps6kb1, indicating focus on myogenesis its indirect impact healing. Notably, 15 shared between groups, consistent trends, suggesting partially overlapping under different mechanical Conclusion findings provided insights into pathways involved regeneration could inform targeted therapeutic strategies enhance

Language: Английский