Gut-testis axis in roosters: Lactiplantibacillus plantarum supplementation improves reproductive performance

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105141 - 105141

Published: April 1, 2025

Probiotics are widely used in poultry farming and industry, as they offer numerous health performance benefits for birds. Probiotic Lactobacilli maintain gut microbiota balance, aid nutrient utilization, boost the immune system, increase stress resistance serve antibiotic alternatives. However, their impact on male reproductive function is not yet fully understood. This study investigated effect of a novel probiotic strain, Lactiplantibacillus plantarum SNI3 (LbSNI3), roosters. Twenty adult roosters were used. LbSNI3 was administered orally (dose: 2 × 107 CFU/animal/day) 7 weeks to half animals. Control birds (10) received sterile tap water vehicle. Ejaculate volume, sperm concentration, motility, number IPVL penetration holes testosterone plasma concentration have been measured weekly. Testis weight, dimensions histology determined at end experiment. mRNA levels select genes, involved spermatogenesis oxidative steroid synthesis testis samples by qRT-PCR. Total antioxidant capacity, superoxide dismutase (SOD) enzyme activity malondialdehyde (MDA) also analyzed. administration increased ejaculate holes, resulting significant improvement reproductivity index. In contrast, statistically different control versus LbSNI3-treated groups. At experiment, size, area, lumen seminiferous tubuli The testicular expression Gpx1, Sepw1, Dio2, Birc5 Rec8 genes elevated following oral LbSNI3. activity, SOD significantly increased, while MDA decreased, indicating enhanced capacity testis. produces bacterial metabolite, γ-glutamyl-glutamate, which enters glutathione cycle strengthens defense mechanisms against stress. conclusion, enhances testis, leading index may be mediated through gut-testis axis could utilized improve productivity livestock industry.

Language: Английский

Salmonella enterica serovar Typhimurium uses T6SS to promote bacterial adaptability and enhance its survival of niches in broiler chickens

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105277 - 105277

Published: May 1, 2025

Language: Английский