Differentially abundant proteins, metabolites, and lipid molecules in spaghetti meat compared to normal chicken breast meat: Multiomics analysis1 DOI Creative Commons
Janghan Choi, Majid Shakeri, Brian Bowker

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105165 - 105165

Published: April 1, 2025

Spaghetti meat (SM), a recently emerging muscle myopathy in chicken breast meat, is characterized by loss of fiber integrity, resulting spaghetti-like appearance. Understanding the differences proteins, metabolites, and lipids through multiomics approach SM can identify its quality traits elucidate exact causes. The purpose this study was to investigate differentially abundant lipid molecules compared normal (Control). supernatant from sample homogenates subjected ultra-high performance liquid chromatography (UHPLC) analysis for multiomic profiling. A total 16 fillets (Pectoralis major) representing Control (n = 8) groups were collected commercial slaughterhouse. 2593 identified composed 1903 506 lipids, 181 compounds 3 electrolytes. There 632 differential 503 76 50 In comparing Control, protein, metabolite, with greatest fold change calponin, decanoylcarnitine, ceramide [N‑hydroxy-sphingosine] (Cer[NS]) d18:1_26:1, respectively. Plasmenylphosphatidylcholine (Plasmenyl-PC) triglycerides (TG) significantly decreased increased, respectively, Control. Acylcarnitines (AC) Decanoylcarnitine, lauroylcarnitine, linoleyl-carnitine, oleoyl-carnitine, hexanoylcarnitine downregulated adenosine 5'-diphosphoribose nicotinamide adenine dinucleotide (NAD) SM. Carbon metabolism, glycolysis/glucogenesis, ribosome, biosynthesis amino acids, aminoacyl-tRNA selected top 10 enriched Kyoto Encyclopedia Genes Genomes (KEGG) pathways, using proteins. Hence, had different related β-oxidation, carbon energy formation, protein acid metabolism Results showed physiological alterations found myopathy. Therefore, mitigate broilers, interventions should: 1) increase NAD carnitines, 2) reduce triglycerides, 3) modulate β-oxidation via nutritional, genetic, or systemic approaches.

Language: Английский

Differentially abundant proteins, metabolites, and lipid molecules in spaghetti meat compared to normal chicken breast meat: Multiomics analysis1 DOI Creative Commons
Janghan Choi, Majid Shakeri, Brian Bowker

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105165 - 105165

Published: April 1, 2025

Spaghetti meat (SM), a recently emerging muscle myopathy in chicken breast meat, is characterized by loss of fiber integrity, resulting spaghetti-like appearance. Understanding the differences proteins, metabolites, and lipids through multiomics approach SM can identify its quality traits elucidate exact causes. The purpose this study was to investigate differentially abundant lipid molecules compared normal (Control). supernatant from sample homogenates subjected ultra-high performance liquid chromatography (UHPLC) analysis for multiomic profiling. A total 16 fillets (Pectoralis major) representing Control (n = 8) groups were collected commercial slaughterhouse. 2593 identified composed 1903 506 lipids, 181 compounds 3 electrolytes. There 632 differential 503 76 50 In comparing Control, protein, metabolite, with greatest fold change calponin, decanoylcarnitine, ceramide [N‑hydroxy-sphingosine] (Cer[NS]) d18:1_26:1, respectively. Plasmenylphosphatidylcholine (Plasmenyl-PC) triglycerides (TG) significantly decreased increased, respectively, Control. Acylcarnitines (AC) Decanoylcarnitine, lauroylcarnitine, linoleyl-carnitine, oleoyl-carnitine, hexanoylcarnitine downregulated adenosine 5'-diphosphoribose nicotinamide adenine dinucleotide (NAD) SM. Carbon metabolism, glycolysis/glucogenesis, ribosome, biosynthesis amino acids, aminoacyl-tRNA selected top 10 enriched Kyoto Encyclopedia Genes Genomes (KEGG) pathways, using proteins. Hence, had different related β-oxidation, carbon energy formation, protein acid metabolism Results showed physiological alterations found myopathy. Therefore, mitigate broilers, interventions should: 1) increase NAD carnitines, 2) reduce triglycerides, 3) modulate β-oxidation via nutritional, genetic, or systemic approaches.

Language: Английский

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