Exploring the functional variations of key candidate genes affecting egg production by hypothalamic-pituitary-ovarian axis in chickens DOI Creative Commons
Dandan Wang,

Yiqian Zhu,

Jiajia Dong

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105027 - 105027

Published: March 1, 2025

Potential genetic variants associated with chicken egg production traits have been extensively identified, most of which are located in the non-coding regions genome. However, functional really drive egg-laying phenotype change remain elusive. In present study, by integrating previously screened related candidate variants, transcriptome data derived from 16 high- and low-yield Gushi chickens, epigenomic analyses, 22 potential (PFVs) were systematically identified. These PFVs potentially differences phenotypes affecting expression 10 key genes hypothalamus (ZNF804B, DPP10, NEO1 GABRG1), pituitary (DPP10 GNG7), ovary (PHIP, OSTN, GADD45B, NFXL1 ADAMTS17). Subsequently, regulatory activity function one PFV, chr3:79510218A>T, third intron pleckstrin homology domain interacting protein gene (PHIP), investigated ovarian granulosa cells. Association analysis confirmed significant association chr3:79510218A>T number 21 to 43 week age (P = 0.0022) Guangxi Yao 0.0388), as well PHIP levels 0.0010). Functional indicated that T allele enhanced transcriptional upregulated vitro binding transcription factor forkhead box I1 (FOXI1). Furthermore, knockdown led inhibition cell proliferation a reduction synthesis secretion progesterone (PROG) hormones. Collectively, this study unveil illustrate regulation mechanism, will help accelerate molecular design breeding process production.

Language: Английский

Exploring the functional variations of key candidate genes affecting egg production by hypothalamic-pituitary-ovarian axis in chickens DOI Creative Commons
Dandan Wang,

Yiqian Zhu,

Jiajia Dong

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105027 - 105027

Published: March 1, 2025

Potential genetic variants associated with chicken egg production traits have been extensively identified, most of which are located in the non-coding regions genome. However, functional really drive egg-laying phenotype change remain elusive. In present study, by integrating previously screened related candidate variants, transcriptome data derived from 16 high- and low-yield Gushi chickens, epigenomic analyses, 22 potential (PFVs) were systematically identified. These PFVs potentially differences phenotypes affecting expression 10 key genes hypothalamus (ZNF804B, DPP10, NEO1 GABRG1), pituitary (DPP10 GNG7), ovary (PHIP, OSTN, GADD45B, NFXL1 ADAMTS17). Subsequently, regulatory activity function one PFV, chr3:79510218A>T, third intron pleckstrin homology domain interacting protein gene (PHIP), investigated ovarian granulosa cells. Association analysis confirmed significant association chr3:79510218A>T number 21 to 43 week age (P = 0.0022) Guangxi Yao 0.0388), as well PHIP levels 0.0010). Functional indicated that T allele enhanced transcriptional upregulated vitro binding transcription factor forkhead box I1 (FOXI1). Furthermore, knockdown led inhibition cell proliferation a reduction synthesis secretion progesterone (PROG) hormones. Collectively, this study unveil illustrate regulation mechanism, will help accelerate molecular design breeding process production.

Language: Английский

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