Simplified Plasmodium falciparum membrane feeding assay using small Petri dishes and gel warmers DOI Creative Commons
Godfree Mlambo,

Tassanee Thanakornsombut,

Abhai K. Tripathi

et al.

Malaria Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Dec. 22, 2024

Abstract Studies on Plasmodium falciparum transmission require blood-feeding infectious gametocytes to mosquitoes using standard membrane-feeding assays (SMFAs). SMFAs are routinely performed electric heating coils or glass membrane feeders connected a circulatory water bath tubing and clamps. Each of these approaches is expensive requires complex setup, hence restricting the number that can be simultaneously. Furthermore, existing methods cannot easily applied in low-resource field settings. This study presents low-cost simplified method for feeding with an blood meal 35 mm Petri dishes where temperature maintained by reusable gel warmers. The intensity prevalence infection ( Anopheles stephensi gambiae ) fed via dish overlaid warmers were comparable feeders. methodology described here settings due its low cost, ease set up, use available supplies, such as dishes, We believe wide range laboratories adapt this P. studies.

Language: Английский

Distribution of Anophelinae (Diptera: Culicidae) and challenges for malaria elimination in Brazil DOI Creative Commons
Maria Anice Mureb Sallum, Thiago Salomão de Azevedo, Jan E. Conn

et al.

Memórias do Instituto Oswaldo Cruz, Journal Year: 2025, Volume and Issue: 120

Published: Jan. 1, 2025

In 1909, Arthur Neiva published an article titled “Contribuição para os estudos dos dipteros. Observação sobre a biolojia e sistematica das anofelinas brasileiras suas relações com o impaludismo”, highlighting the biology, ecology, and distribution of Anophelinae mosquitoes need for more taxonomic studies in Brazil. This came 11 years after Ronald Ross Grassi demonstrated mosquito roles transmitting Plasmodium to birds humans. Despite considerable advances understanding species, knowledge remains insufficient given complexity Brazil’s ecosystems, intensified anthropogenic environmental changes since mid-20th century, persistent public health challenges posed by malaria. perspective presents vectors potential vector species Brazil using climate variables maximum entropy model. Geographical maps including putative are provided. The also discusses current relation malaria elimination plan, along with ecological factors influencing distribution.

Language: Английский

Citations

0
Anopheles gambiae phagocytic hemocytes promote Plasmodium falciparum infection by regulating midgut epithelial integrity DOI Creative Commons
Víctor Cardoso-Jaime, George Dimopoulos

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 8, 2025

For successful transmission, the malaria parasite must traverse tissue epithelia and survive attack from insect's innate immune system. Hemocytes play a multitude of roles in mosquitoes, including defense against invading pathogens. Here, we show that hemocytes major vector Anopheles gambiae promote Plasmodium falciparum infection by maintaining midgut epithelial integrity controlling cell proliferation upon blood feeding. The mosquito's also control microbiota gene expression. Our study unveils novel hemocyte functions are exploited human to evade Cardoso Dimopoulos mosquito macrophages-like cells agonists early stages critical role

Language: Английский

Citations

0
Advances in the dissection of Anopheles–Plasmodium interactions DOI Creative Commons
Sally A. Saab, Víctor Cardoso-Jaime, Mary Kefi

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(3), P. e1012965 - e1012965

Published: March 31, 2025

Malaria is a life-threatening mosquito-borne disease caused by the Plasmodium parasite, responsible for more than half million deaths annually and principally involving children. The successful transmission of malaria Anopheles mosquitoes relies on complex successive interactions between parasite various mosquito organs, host factors, restriction factors. This review summarizes our current understanding mechanisms regulating infection vector at plasmodial developmental stages highlights potential transmission-blocking targets strategies.

Language: Английский

Citations

0
Establishing a dominant early larval sex-selection strain in the Asian malaria vector Anopheles stephensi DOI Creative Commons
Shih‐Che Weng, Fangying Chen, Ming Li

et al.

Infectious Diseases of Poverty, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 11, 2024

Genetic biocontrol interventions targeting mosquito-borne diseases require the release of male mosquitoes exclusively, as only females consume blood and transmit pathogens. Releasing males eliminates risk increasing mosquito bites spreading pathogens while enabling effective population control. The aim this study is to develop robust sex-sorting methods for early larval stages in mosquitoes, scalable male-only releases genetic interventions.

Language: Английский

Citations

2
Establishing a Male-Positive Genetic Sexing Strain in the Asian Malaria Vector Anopheles stephensi DOI
Shih‐Che Weng, Fangying Chen, Ming Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Genetic biocontrol interventions targeting mosquito-borne diseases require the release of male mosquitoes exclusively, as only females consume blood and transmit human pathogens. This reduces risk spreading pathogens while enabling effective population control. Robust sex sorting methods to enable early larval in need be developed allow for scalable genetic interventions. study applies SEPARATOR (Sexing Element Produced by Alternative RNA-splicing A Transgenic Observable Reporter) system, previously

Language: Английский

Citations

1
Beyond immunity: a transcriptomic landscape of Plasmodium’s modulation of mosquito metabolic pathways DOI Creative Commons
Luz García‐Longoria, Arnaud Berthomieu, Olof Hellgren

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Abstract The focus of mosquito- Plasmodium interactions has predominantly been centered on mosquito immunity, revealing key mechanisms by which mosquitoes attempt to combat infection. However, recent evidence suggests that beyond a multitude physiological and metabolic pathways play crucial roles in determining whether the parasite completes its development within mosquito. We review these are potentially modulated , fragmented occasionally contradictory state knowledge. then present comprehensive transcriptomic analysis -infected uninfected mosquitoes, examining gene expression genes across different stages parasite’s development. These range from enzymes proteins involved gut structure function, egg production resorption, salivary gland invasion behaviour. For this purpose, we use non-model system consisting avian malaria relictum an invasive threatening bird biodiversity world, natural vector, Culex pipiens . Our results reveal how at each stage mosquito, modulates myriad pathways, ways favour survival completion life cycle. discuss constitutes sufficient parasite-driven manipulation or changes simply mosquito’s response infection, may serendipitously exploit enhance fitness. study extends comparative analyses malaria-infected human rodent parasites, provides insights into degree conservation selective pressures exerted parasites their vectors.

Language: Английский

Citations

0
A novel broad-spectrum antibacterial and anti-malarial Anopheles gambiae Cecropin promotes microbial clearance during pupation DOI Creative Commons
Cairé Barreto, Víctor Cardoso-Jaime, George Dimopoulos

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(10), P. e1012652 - e1012652

Published: Oct. 23, 2024

Anophelinae mosquitoes are exposed to a variety of microbes including Plasmodium parasites that cause malaria. When infected, mount versatile immune responses, the production antimicrobial peptides. Cecropins one most widely distributed families peptides in insects and all previously studied Anopheles members playing roles adult mosquito immunity. We have identified characterized novel member gambiae cecropin family, D (CecD), is uniquely expressed immune-responsive at late larval stages promote microbial clearance through its broad-spectrum antibacterial activity during larval-pupal developmental transition. Interestingly, Cecropin also exhibited highly potent against falciparum sporozoites, malaria parasite stage transmitted from infects humans thereby holds promise as transmission-blocking agent. Finally, we defined unequivocal cecropin-specific molecular signatures systematically organize diversity family vectors.

Language: Английский

Citations

0
Simplified P. falciparum membrane feeding assay using small Petri dishes and gel warmers DOI Creative Commons
Godfree Mlambo,

Tassanee Thanakornsombut,

K Tripathi Abhai

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Studies on Plasmodium falciparum transmission require blood-feeding infectious gametocytes to mosquitoes using standard membrane-feeding assays (SMFAs). SMFAs are routinely performed electric heating coils or glass membrane feeders connected a circulatory water bath tubing and clamps. Each of these approaches is expensive requires complex setup, hence restricting the number that can be simultaneously. Furthermore, existing methods cannot easily applied in low-resource field settings. Here we describe low-cost simplified method for feeding with an blood meal 35 mm Petri dishes where temperature maintained by placing reusable gel warmers top dishes. The intensity prevalence infection (An. stephensi An. gambiae) fed via dish overlaid were comparable feeders. methodology described here settings due its low cost, ease set up, use available supplies such as petri dishes, warmers. We believe wide range laboratories adapt this P. studies.

Language: Английский

Citations

0
Genomic basis of schistosome resistance in a molluscan vector of human schistosomiasis DOI Creative Commons

Si‐Ming Zhang,

Guiyun Yan,

Abdelmalek Lekired

et al.

iScience, Journal Year: 2024, Volume and Issue: 28(1), P. 111520 - 111520

Published: Dec. 2, 2024

Freshwater snails are obligate intermediate hosts for the transmission of schistosomiasis, one world's most devastating parasitic diseases. To decipher mechanisms underlying snail resistance to schistosomes, recombinant inbred lines (RILs) were developed from two well-defined homozygous (iM line and iBS90)

Language: Английский

Citations

0
Simplified Plasmodium falciparum membrane feeding assay using small Petri dishes and gel warmers DOI Creative Commons
Godfree Mlambo,

Tassanee Thanakornsombut,

Abhai K. Tripathi

et al.

Malaria Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Dec. 22, 2024

Abstract Studies on Plasmodium falciparum transmission require blood-feeding infectious gametocytes to mosquitoes using standard membrane-feeding assays (SMFAs). SMFAs are routinely performed electric heating coils or glass membrane feeders connected a circulatory water bath tubing and clamps. Each of these approaches is expensive requires complex setup, hence restricting the number that can be simultaneously. Furthermore, existing methods cannot easily applied in low-resource field settings. This study presents low-cost simplified method for feeding with an blood meal 35 mm Petri dishes where temperature maintained by reusable gel warmers. The intensity prevalence infection ( Anopheles stephensi gambiae ) fed via dish overlaid warmers were comparable feeders. methodology described here settings due its low cost, ease set up, use available supplies, such as dishes, We believe wide range laboratories adapt this P. studies.

Language: Английский

Citations

0