Small molecule probes for peroxynitrite detection

Redox Biochemistry and Chemistry, Journal Year: 2024, Volume and Issue: 10, P. 100034 - 100034

Published: July 26, 2024

Peroxynitrite (ONOO‒/ONOOH) is a short-lived but highly reactive species that formed in the diffusion-controlled reaction between nitric oxide and superoxide radical anion. It can oxidize certain biomolecules has been considered as key cellular oxidant under various pathophysiological conditions. crucial to selectively detect quantify ONOO– determine its role biological processes. In this review, we discuss approaches used ONOO‒ cell-free systems with major emphasis on small-molecule chemical probes. We review principles mechanisms responsible for formation of detectable products, plausible limitations recommend use boronate-based probes ONOO‒, they react directly rapidly ONOO–, produce minor ONOO‒‒specific kinetics mechanism have rigorously characterized. Specific experimental protocols detection cell-free, cellular, vivo using molecular are provided (as shown BOX 1, 2, 3, 4, 5, 6).

Language: Английский

Special issue on “Peroxynitrite and Reactive Nitrogen Species” dedicated to the 25th Anniversary of the Nitric Oxide Nobel Prize

Redox Biochemistry and Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 100044 - 100044

Published: Nov. 1, 2024

Language: Английский