Quantitative analysis of the proteome and protein oxidative modifications in primary human coronary artery endothelial cells and associated extracellular matrix

Redox Biology, Journal Year: 2025, Volume and Issue: 81, P. 103524 - 103524

Published: Feb. 7, 2025

Vascular endothelial cells (ECs) play a key role in physiology by controlling arterial contraction and relaxation, molecular transport. EC dysfunction is associated with multiple pathologies. Here, we characterize the cellular extracellular matrix (ECM) proteomes of primary human coronary artery ECs, from donors, oxidation/nitration products formed on these during cell culture, using liquid chromatography-mass spectrometry. In total ∼9900 proteins were identified 3 ∼7000 per donor. Of ∼5300 consistently identified, indicating some heterogeneity across age possible cause. Multiple endogenous oxidation detected both ECM (and particularly endoplasmic reticulum species). contrast, nitration was mostly cytoskeletal proteins, consistent intracellular generation nitrating agents, possibly nitric oxide synthase (eNOS) or peroxidase enzymes. The modifications are ascribed to physiological enzymatic activity (hydroxylation at proline/lysine; predominantly especially collagens) formation reactive species (oxidation tryptophan/tyrosine/histidine; tryptophan/tyrosine). sites present limited number peptides (104 oxidized; 23 nitrated) modest proteins. A small modifications, being selective specific targets. Several nitrated all also smooth muscle suggesting that major targets vascular proteome. These data provide 'background' proteome dataset for studies disease.

Language: Английский

Special issue on “Peroxynitrite and Reactive Nitrogen Species” dedicated to the 25th Anniversary of the Nitric Oxide Nobel Prize

Redox Biochemistry and Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 100044 - 100044

Published: Nov. 1, 2024

Language: Английский