Viral inactivation of murine coronavirus via multiple gas plasma-derived reactive species
Redox Biology,
Journal Year:
2025,
Volume and Issue:
82, P. 103591 - 103591
Published: March 11, 2025
The
recent
pandemic
has
highlighted
the
urgent
need
to
elucidate
pathophysiological
mechanisms
underlying
viral
effects
in
humans
and
is
driving
search
for
innovative
antiviral
therapies.
Several
studies
have
investigated
ability
of
gas
plasma,
a
partially
ionized
that
simultaneously
generates
several
reactive
species,
be
new
tool.
However,
aspects
action
plasma
remained
elusive.
In
this
study,
we,
first
time,
used
device
approved
medical
purposes
routinely
applied
clinics,
especially
wound
healing,
test
its
activity
against
murine
corona-virus
vitro
(MHV-GFP),
research
model
analogous
human
coronaviruses
such
as
SARS-CoV-2.
For
this,
we
established
novel
high-content
imaging
assay
gave
quantitative
kinetic
information
about
infection
reduced
fibroblasts
(17Cl-1)
host
cells.
Gas
treatment
delayed
infectivity
overall
toxicity
17Cl1
Various
antioxidants
at
different
concentrations
were
screened
identify
ROS
relevant
effects.
Catalase
provided
no
virus
protection,
DMSO,
mannitol,
histidine,
Trolox,
ascorbic
acid
only
modestly
virucidal
efficacy.
By
contrast,
glutathione,
tyrosine,
cysteine
showed
profound
but
not
complete
protection
MHV
from
plasma-derived
suggesting
pivotal
roles
superoxide
radicals
peroxynitrite
plasma-driven
inactivation.
At
extended
exposure
times,
fewer
intact
RNA
detected,
indicative
species-driven
modifications
or
degradation
an
additional
mechanism
action.
Virus
particle
size
changes
measured
by
electron
microscopy
moderate.
Collectively,
identified
potent
clinically
argon
jet
along
with
potential
Language: Английский
Special issue on “Peroxynitrite and Reactive Nitrogen Species” dedicated to the 25th Anniversary of the Nitric Oxide Nobel Prize
Redox Biochemistry and Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown, P. 100044 - 100044
Published: Nov. 1, 2024
Language: Английский
Hole Transfer and the Resulting DNA Damage
Biomolecules,
Journal Year:
2024,
Volume and Issue:
15(1), P. 29 - 29
Published: Dec. 30, 2024
In
this
review,
we
focus
on
the
one-electron
oxidation
of
DNA,
which
is
a
multipart
event
controlled
by
several
competing
factors.
We
will
discuss
free
energies
four
nucleobases
and
electron
detachment
from
influenced
specific
interactions
like
hydrogen
bonding
stacking
with
neighboring
sites
in
double
strand.
The
formation
radical
cation
(hole)
can
migrate
through
DNA
(hole
transport),
depending
sequence-specific
effects
allocation
final
oxidative
damage,
also
addressed.
Particular
attention
given
to
ds-ODN
containing
G:C
pairs,
including
complex
mechanism
deprotonation
vs.
hydration
steps
G:C•+
pair,
as
well
modes
two
guanyl
tautomers
after
deprotonation.
Among
reactive
oxygen
species
(ROS)
generated
aerobic
organisms
cellular
metabolisms,
oxidants
react
DNA.
stable
product
their
use
biomarkers
guanine
damage
are
Language: Английский