Superoxide Dismutase‐Like Regulated Fe/Ppa@PDA/B for Synergistically Targeting Ferroptosis/Apoptosis to Enhance Anti‐Tumor Efficacy

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(29)

Published: July 24, 2023

The cell apoptosis pathway of sonodynamic therapy (SDT) is usually blocked, resulting in limited therapeutic efficacy, therefore, the development new methods for sensitizing targeted ferroptosis and promoting great significance to improve anti-tumor efficacy SDT. Herein, mesoporous Fe3 O4 nanoparticles (NPs) are synthesized loading pyropheophorbide-a (ppa), surface-coated by polydopamine (PDA) further anchored with tumor-targeting moieties biotin obtain Fe/ppa@PDA/B NPs. displayes pH/ultrasound (US) responsive release properties, magnetic resonance imaging (MRI) functions. Moreover, NPs as Fe source ferroptosis, enhances sensitivity consuming glutathione (GSH) producing hydroxyl radical (OH). quinone groups PDA layer on own free electrons, which led effective superoxide dismutase (SOD) action through anion (O2- ) disproportionation hydrogen peroxide (H2 O2 oxygen (O2 ), thus, overcame hypoxia SDT promoted ·OH generation ions under US trigger, synergistically improves enhance both vitro vivo. strategy synergistic induce GSH depletion self-sufficient regulated SOD provides a idea enhancing efficacy.

Language: Английский

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Effects of Cryptocaryon irritans infection on the histopathology, oxidative stress, immune response, and intestinal microbiota in the orange-spotted grouper Epinephelus coioides

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 133, P. 108562 - 108562

Published: Jan. 20, 2023

Language: Английский

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In Situ and Real-Time Monitoring of Mitochondria–Endoplasmic Reticulum Crosstalk in Apoptosis via Surface-Enhanced Resonance Raman Spectroscopy

Nano Letters, Journal Year: 2023, Volume and Issue: 23(17), P. 8363 - 8369

Published: Aug. 23, 2023

The crosstalk between mitochondria and endoplasmic reticula plays a crucial role in apoptotic pathways which reactive oxygen species (ROS) produced by microsomal monooxygenase (MMO) are believed to accelerate cytochrome c release. Herein, we successfully demonstrate the potential of surface-enhanced resonance Raman spectroscopy (SERRS) for monitoring MMO-derived ROS formation ROS-mediated Silver nanoparticles coated with nickel shells used as both signal enhancers electron donors c. SERRS is found be sensitive ROS, allowing situ probing cell death inducer. Label-free evaluation ROS-induced apoptosis achieved SERRS-based release living cells. This study verifies capability label-free, situ, real-time mitochondria–endoplasmic reticulum provides novel strategy rational design screening ROS-inducing drugs cancer treatment.

Language: Английский

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Mitochondria-Specific Molecular Crosstalk between Ferroptosis and Apoptosis Revealed by In Situ Raman Spectroscopy

Nano Letters, Journal Year: 2024, Volume and Issue: 24(7), P. 2384 - 2391

Published: Feb. 11, 2024

Ferroptosis and apoptosis are two types of regulated cell death that closely associated with the pathophysiological processes many diseases. The significance ferroptosis–apoptosis crosstalk in fate determination has been reported, but underlying molecular mechanisms poorly understood. Herein mitochondria-mediated is explored. Based on a comprehensive spectroscopic investigation mass spectrometry, cytochrome c-involved Fenton-like reactions lipid peroxidation revealed. More importantly, c found to induce ROS-independent cardiolipin-specific depending its redox state. In situ Raman spectroscopy unveiled erastin can interrupt membrane permeability, specifically through cardiolipin, facilitating release from mitochondria. Details erastin–cardiolipin interaction determined using dynamics simulations. This study provides novel insights into how occurs around mitochondrial membranes trigger ferroptosis apoptosis, significant implications for rational design mitochondria-targeted reducers cancer therapy.

Language: Английский

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pH-responsive berberine release from metal-organic framework based nanocarriers by regulating oxidative stress for targeted therapy of oral squamous cell carcinoma

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(9), P. 105935 - 105935

Published: July 26, 2024

Excessive generation of reactive oxygen species (ROS) induces cellular oxidative stress damage, resulting in mitochondrial dysfunction and subsequent promotion apoptosis. Induction damage through chemo-dynamic therapy within the tumor microenvironment (TME) represents a promising therapeutic strategy for cancer treatment. Herein, folic acid-polyethylene glycol (FA-PEG)-modified MIL-101 NPs loaded with berberine (BER) were constructed to develop nanoplatform based on modulation treatment Oral squamous cell carcinoma (OSCC). Comprehensive characterizations TEM, DLS, XRD, FTIR, TGA UV–vis spectroscopy confirmed successful synthesis MIL-101/PEG-FA uniform size, high drug loading efficiency (32.59 %) superior pH-responsive release (Ber 24.44 % 70.22 96h at pH 7.4 5.0, respectively). Cellular experiments revealed that achieved pH-responsiveness BER TME, thereby improving bioavailability BER. Moreover, Fe3+ MIL-101(Fe) showed strong ability consume GSH provide continuous supply H2O2, which decreased SOD activity, contributed MDA, increasing production toxic ROS CAL27 cells. Meanwhile, MIL-101@BER/PEG-FA up-regulated inflammatory cytokine levels (TNF-α IL-1β), promoted response induced cells apoptosis by regulating LKB1/AMPK pathway. Finally, good against OSCC vivo. Consequently, can be applied as nanocarrier platform OSCC.

Language: Английский

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Self‐Assembly Hypoxic and ROS Dual Response Nano Prodrug as a New Therapeutic Approach for Glaucoma Treatments

Advanced Science, Journal Year: 2024, Volume and Issue: 11(41)

Published: Sept. 4, 2024

Abstract Glaucoma is an irreversible blinding eye disease characterized by retinal ganglion cell (RGC) death.Previous studies have demonstrated that protecting mitochondria and activating the CaMKII/CREB signaling pathway can effectively protect RGC axon. However, currently treatments are often unsatisfactory, pathogenesis of glaucoma requires further elucidation. In this study, a ROS‐responsive dual drug conjugate (OLN monomer) first designed simultaneously bonds nicotinamide oleic acid. The self‐assembled into nanoparticles (uhOLN‐NPs) through aggregation multiple micelles possesses ROS scavenging capability. Then, polymer with hypoxic response function designed, which encapsulates uhOLN‐NPs to form responses (HOLN‐NPs). Under hypoxia in RGCs, azo bond HOLN‐NPs breaks releases uhOLN‐NPs. Meanwhile, under high conditions, thioketone broke, leading dissociation nano‐prodrug. released acid co‐scavenge activate pathway, RGCs. exhibit significantly superior protective effect on R28 cells glutamate models glaucoma. accumulation RGCs lead significant inhibition apoptosis axonal damage vivo. Notably, provide new therapeutic approach for patients neurodegenerative disease.

Language: Английский

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Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 13868 - 13868

Published: Nov. 10, 2022

Surface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS useful probing intrinsic information proteins attracting increasing attention in biophysics, bioanalytical chemistry, biomedicine. This review starts with a brief introduction theories methodology protein characterization. SERS-active materials, related synthetic approaches, strategies protein-material assemblies are outlined discussed, followed by detailed discussion without cofactors. Recent applications advances biomarker detection, cell analysis, pathogen discrimination then highlighted, the spectral reproducibility limitations critically discussed. The ends conclusion current challenges perspectives promising directions.

Language: Английский

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Monitoring of Propiolamide-Mediated Molecular Crosstalk between Ferroptosis and Apoptosis by Raman Spectroscopy

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Inducing programmed cell death is an efficient strategy for cancer treatments, and a deep understanding of the molecular mechanisms underlying pathways significance rational design anticancer drugs. Herein, propiolamide-mediated crosstalk between ferroptosis apoptosis investigated. In situ monitoring reactive oxygen species (ROS) formation structural changes propiolamide compounds achieved by Raman spectroscopy. The propiolamides in inducing monooxygenase-mediated ROS generation inhibiting GPX4 activities are revealed. Furthermore, pro-ferroptotic pro-apoptotic roles containing terminal alkynes verified. This study provides label-free enzyme-drug interactions their dynamics. It first attempt to basis through two important enzymes death. paves way designing novel drugs that capable triggering synergistic contribution multiple forms efficacy.

Language: Английский

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Triphenyl Phosphonium Functionalized Amphiphilic Peptides as Promising Antibacterial and Anticancer Agents

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Triphenyl phosphonium conjugated amphiphilic peptides are self-assembled into micelles that show potential antimicrobial activity against drug resistant strains.

Language: Английский

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Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2123 - 2123

Published: Dec. 16, 2023

After parturition, bovine endometrial epithelial cells (BEECs) undergo serious inflammation and imbalance between oxidation antioxidation, which is widely acknowledged as a primary contributor to the development of endometritis in dairy cows. Nevertheless, mechanism oxidative stress-mediated damage remains inadequately defined, particularly molecular pathways associated with mitochondria-dependent apoptosis. Hence, present study was designed explore responsible for mitochondrial dysfunction-induced BEEC damage. In vivo, expressions proapoptotic protein caspase 3 cytochrome C were increased significantly uteri endometritis. Similarly, levels 3, BAX, markedly H2O2-treated BEECs. Our findings revealed pronounced cows endometritis, accompanied by heightened expression cyto-C caspase-3 both vivo vitro. The reduction apoptosis-related BEECs due oxidant injury notably mitigated following N-acetyl-L-cysteine (NAC) treatment. Furthermore, vacuolation alleviated, membrane potential returned normal after removal ROS. Excessive ROS may be main cause dysfunction. Mitochondrial permeability transition pore (mPTP) blockade cyclophilin D (CypD) knockdown CSA blocked flow (cyto-C) Ca2+ cytoplasm from mitochondria. results indicate that elevated persistent opening mPTP are causes Collectively our reveal new involving ROS-mPTP signaling BEECs, avenue clinical treatment

Language: Английский

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