Gut microbiome and metabolites mediate the benefits of caloric restriction in mice after acute kidney injury DOI Creative Commons
Xuexue Zhu, Xiao Fu, Xinyu Meng

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 77, P. 103373 - 103373

Published: Sept. 27, 2024

The role of gut microbiome in acute kidney injury (AKI) is increasing recognized. Caloric restriction (CR) has been shown to enhance the resistance ischemia/reperfusion kidneys rodents. Nonetheless, it unknown whether intestinal microbiota mediated CR protection against ischemic/reperfusion-induced (IRI) kidneys. Herein, we showed that ameliorated IRI-elicited renal dysfunction, oxidative stress, apoptosis, and inflammation, along with enhanced barrier function. In addition, depletion blocked favorable effects AKI mice. 16S rRNA metabolomics analysis enriched commensal Parabacteroides goldsteinii (P. goldsteinii) upregulated level serum metabolite dodecafluorpentan. Intestinal colonization P. oral administration dodecafluorpentan similar beneficial as RNA sequencing experimental data revealed protected AKI-induced by antagonizing burst NFκB-induced NLRP3 inflammasome activation. screened found Hamaudol improved insufficiency boosting growth goldsteinii. Our results shed light on metabolites benefits AKI.

Language: Английский

Network pharmacology, molecular docking, and experimental verification reveal the mechanism of Yi-Shen-Hua-Shi granules treating acute kidney injury DOI
Sheng Zhang,

Jiankui Du,

Minmin Lu

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119320 - 119320

Published: Jan. 1, 2025

Language: Английский

Citations

0
Panax Notoginseng Saponins Inhibit Apoptosis and Alleviate Renal Ischemia–Reperfusion Injury Through the ROCK2/NF-κB Pathway DOI
Xin Liu,

Ning Kanghao,

Jiacheng Li

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

Citations

0
Perillaldehyde ameliorates sepsis-associated acute kidney injury via inhibiting HSP90AA1-mediated ferroptosis and pyroptosis: Molecular structure and protein interaction of HSP90AA1 DOI
Shuai Liu, Yunfei Xu, Xudong Yao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 304, P. 140954 - 140954

Published: Feb. 11, 2025

Language: Английский

Citations

0
PU.1/Spi1 exacerbates ischemia-reperfusion induced acute kidney injury via upregulating Gata2 and promoting fibroblast activation DOI
Chen Zong, Geliang Xu, Ming Ning

et al.

Acta Pharmacologica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Sodium aescinate protects renal ischemia-reperfusion and pyroptosis through AKT/NLRP3 signaling pathway DOI Creative Commons
Xin Liu,

Ning Kanghao,

Jiacheng Li

et al.

Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)

Published: April 22, 2025

Renal ischemia-reperfusion injury (RIRI) is a common cause of acute renal injury. Studies have shown that sodium aescinate (SA) may serve as potential therapeutic agent, although its exact mechanism remains unclear. This study first evaluated the efficacy SA using mouse model. Subsequently, was elucidated through systematic bioinformatics, and finally validated in vitro vivo experiments. The results demonstrated has protective effect on function mice with RIRI. Bioinformatic analysis indicated pyroptosis pathway significantly activated during injury, immunohistochemistry showed level upregulated Administration able to reduce expression pyroptosis-related proteins (GSDMD, NLRP3, IL-1β) In experiments further confirmed exerts an anti-pyroptotic by inhibiting AKT/NLRP3 signaling pathway. Ultimately, mitigates kidney IRI suppressing failure inhibition

Language: Английский

Citations

0
Dapagliflozin ameliorates kidney injury following limb ischemia-reperfusion via the AMPK/SIRT1/NLRP3 pathway DOI Creative Commons

Qiuxiao Zhu,

Huiyao Hao,

Ya Gao

et al.

Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)

Published: April 23, 2025

Limb ischemia-reperfusion (I/R) results in both localized tissue harm and injury to distant organs, particularly affecting the kidneys leading acute kidney injury. This study evaluates renoprotective effect of dapagliflozin, a drug frequently prescribed for type 2 diabetes management, relation caused by limb I/R. The extent was detected through serum marker testing rat model. Oxidative stress indicators inflammatory factors were evaluated cellular models. Histological changes examined using HE staining electron microscopy. Cell pyroptosis quantified TUNEL flow cytometry. Cellular mitochondrial function analyzed with JC-1 staining. AMPK/SIRT1/NLRP3 pathway-related proteins their mRNAs assessed via western blotting RT-qPCR techniques. We showed that dapagliflozin reduced CRE, BUN, NGAL KIM-1 levels improved renal pathology rat. Additionally, significantly raised concentrations GSH-Px SOD, concurrently MDA ROS vivo vitro. It also lowered IL-6 TNF-α cell pyroptosis. Furthermore, it observed elevated AMPK SIRT1 expressions, while decreasing NLRP3, ASC, GSDMD, IL-1β, caspase-1 expressions. Notably, these effects diminished presence siRNA. Taken together, exhibits significant protective against resulting from operates inhibition activating signaling pathway.

Language: Английский

Citations

0
The Spatiotemporal and Paradoxical Roles of NRF2 in Renal Toxicity and Kidney Diseases DOI Creative Commons
Yiying Bian,

Jize Dong,

Zhengsheng Zhou

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 79, P. 103476 - 103476

Published: Dec. 19, 2024

Language: Английский

Citations

3
Pathophysiological role and potential drug target of NLRP3 inflammasome in the metabolic disorders DOI Creative Commons
Huiming Hu, Shuwen Wang, Chen Chen

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: 122, P. 111320 - 111320

Published: July 26, 2024

NLRP3 plays a role in the development of autoinflammatory diseases. NLRP3, ASC, and Caspases 1 or 8 make up inflammasome, which is an important part innate immune system. The inflammasome-mediated inflammatory cytokines may also participate metabolic disorders, such as diabetes, hyperlipidemia, atherosclerosis, non-alcoholic fatty liver disease, gout. Hence, overview regulation these diseases potential drugs targeting focus this review.

Language: Английский

Citations

2
Effusol ameliorates ischemic stroke by targeting NLRP3 protein to regulate NLRP3 inflammasome-mediated pyroptosis DOI
Libin Xu, Siyu Li,

Jiaxin Qi

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 136, P. 156253 - 156253

Published: Nov. 25, 2024

Language: Английский

Citations

2
Gut microbiome and metabolites mediate the benefits of caloric restriction in mice after acute kidney injury DOI Creative Commons
Xuexue Zhu, Xiao Fu, Xinyu Meng

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 77, P. 103373 - 103373

Published: Sept. 27, 2024

The role of gut microbiome in acute kidney injury (AKI) is increasing recognized. Caloric restriction (CR) has been shown to enhance the resistance ischemia/reperfusion kidneys rodents. Nonetheless, it unknown whether intestinal microbiota mediated CR protection against ischemic/reperfusion-induced (IRI) kidneys. Herein, we showed that ameliorated IRI-elicited renal dysfunction, oxidative stress, apoptosis, and inflammation, along with enhanced barrier function. In addition, depletion blocked favorable effects AKI mice. 16S rRNA metabolomics analysis enriched commensal Parabacteroides goldsteinii (P. goldsteinii) upregulated level serum metabolite dodecafluorpentan. Intestinal colonization P. oral administration dodecafluorpentan similar beneficial as RNA sequencing experimental data revealed protected AKI-induced by antagonizing burst NFκB-induced NLRP3 inflammasome activation. screened found Hamaudol improved insufficiency boosting growth goldsteinii. Our results shed light on metabolites benefits AKI.

Language: Английский

Citations

1