A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Ferroptosis and PANoptosis Under Hypoxia Pivoting on the Crosstalk between DHODH and GPX4 in Corneal Epithelium DOI
Ming‐Feng Wu, Xi Peng, Mingchang Zhang

et al.

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: 228, P. 173 - 182

Published: Jan. 5, 2025

Language: Английский

Citations

2
Phytic Acid Extraction from Rice Bran with Acetic Acid : Optimization, Antioxidant Capacity and Mechanism Based on Cellular Metabolomics DOI Creative Commons
Yue Chen, Ruyi Sha, Jing Dai

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117394 - 117394

Published: Jan. 1, 2025

Language: Английский

Citations

0
Hybrid hydrogel based on porcine-derived matrix with gallic acid and cerium-doped mesoporous bioactive glass for diabetic wound healing DOI Creative Commons

Haozeng,

Qinghong Lai,

Wanyou Liao

et al.

Materials & Design, Journal Year: 2025, Volume and Issue: unknown, P. 113714 - 113714

Published: Feb. 1, 2025

Language: Английский

Citations

0
A calcitonin gene-related peptide co-crosslinked hydrogel promotes diabetic wound healing by regulating M2 macrophage polarization and angiogenesis DOI Creative Commons
Xiangyu Li,

Min Yi,

Ziyan Song

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Delayed diabetic wound (DBW) healing is a severe complication of diabetes, characterized notably by peripheral sensory neuropathy. The underlying mechanism nerves and DBW remain unclear. Here, we demonstrate the role calcitonin gene-related peptide (CGRP) in regulating epithelialization angiogenesis DBW. Subsequently, design synthesis gelatin methacryloyl (GelMA-CGRP) hydrogel that slowly releases CGRP, evaluated its effect on promoting healing. results show CGRP abnormally downregulated DBW, ablation further delays This due to reduced M2 polarization decreased absence whereas local application GelMA-CGRP accelerates Mechanistic studies indicate promotes macrophage inhibiting p53 signaling pathway enhances endothelial cell function, thereby accelerating These findings suggest could provide novel therapeutic approach for treatment. STATEMENT OF SIGNIFICANCE: Current methods treating wounds have many limitations. Compared conventional dressings, hydrogels combined with drugs or biological factors promote become an important research direction recent years. study reveals key pathogenesis wounds. found pathway, We utilized carrier properties GelMA develop material delivers effectively treats demonstrates strong biocompatibility antimicrobial properties, offering treatment

Language: Английский

Citations

0
A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Citations

1