Bone marrow mesenchymal stem cells transport connexin43 via tunneling nanotubes to alleviate isopreterenol-induced myocardial hypertrophy

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 6, 2025

Paracrine signaling plays an important role in stem cell therapy. However, it alone cannot fully explain the therapeutic mechanisms of therapy treating heart diseases. Recently, tunneling nanotubes (TNTs)-a novel type long-distance intercellular connectional structure-have been identified between mesenchymal cells (MSCs) and cardiomyocytes (CMs). TNTs mediate transmission multiple molecules, enabling to exert different biological functions. In present study, we investigated MSC-based for myocardial hypertrophy. MSCs And CMs were co Cultured 24 h with or without isopreterenol (ISO) induce Confocal microscopy was used quantify analyze number, morphology, composition, source CMs. effects ISO on assessed by comparing area (measured confocal microscopy) expression levels hypertrophy Related genes (using qRT PCR) under Culture trans Well culture conditions. Flow cytometry employed assess transfer connexin43 (Cx43) from CMs; lentivirus Mediated Cx43 overexpression siRNA investigate Induced stimulation significantly increased length, thickness (Number: P < 0.05; length thickness: 0.01). also proportion containing microtubules those derived (P 0.05). Co-culture conditions more effective than trans-well alleviating ISO-induced Furthermore, observed TNTs, enhanced Cx43-mCherry co-cultured Overexpression alleviated hypertrophy, whereas knocking down reduced their ability alleviate Our results demonstrate that promotes formation particularly CMs, induces changes morphology (thickening lengthening). Additionally, transmitted via which contributes alleviation These suggest represent mechanism MSC-mediated

Language: Английский

One more piece in the puzzle of stem cell therapy in cardiovascular diseases

Revista Portuguesa de Cardiologia, Journal Year: 2024, Volume and Issue: 43(12), P. 657 - 658

Published: Aug. 28, 2024

Language: Английский