Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates DOI Creative Commons
Alexa Rabeling, Mubeen Goolam

FASEB BioAdvances, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

ABSTRACT Stem cell‐based models of embryogenesis have exploded in popularity, resulting protocols with overlapping use some reagents and differential others. As such, the precise contributions individual signaling molecules, such as Chiron BMP4, applied to whole or part aggregates, matrices, Matrigel, development these are unknown. Furthermore, different methods, matrices has yet be directly compared under same conditions. In this paper, we used a mouse embryonic stem cell aggregate model compare BMP4 pulses (applied aggregate) centers embedded them low‐percentage Matrigel. Each factor had effects on morphology, Brachyury protein expression, lineage commitment, having pulses. pulse was shown drive neural differentiation, while resulted better recapitulation aspects anterior–posterior axis formation, polarization expression anterior posterior genes observed. This further elucidates inform decisions around experimental conditions for vitro development.

Language: Английский

Three‐dimensional stem cell models of mammalian gastrulation DOI Creative Commons
David A. Turner, Alfonso Martínez Arias

BioEssays, Journal Year: 2024, Volume and Issue: 46(12)

Published: Aug. 28, 2024

Abstract Gastrulation is a key milestone in the development of an organism. It period cell proliferation and coordinated cellular rearrangement, that creates outline body plan. Our current understanding mammalian gastrulation has been improved by embryo culture, but there are still many open questions difficult to address because intrauterine embryos low number specimens. In case humans, additional difficulties associated with technical ethical challenges. Over last few years, pluripotent stem models being developed have potential become useful tools understand gastrulation. Here we review these special emphasis on gastruloids provide survey methods produce them robustly, their uses, relationship embryos, prospects as well limitations.

Language: Английский

Citations

8
Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates DOI Creative Commons
Alexa Rabeling, Mubeen Goolam

FASEB BioAdvances, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

ABSTRACT Stem cell‐based models of embryogenesis have exploded in popularity, resulting protocols with overlapping use some reagents and differential others. As such, the precise contributions individual signaling molecules, such as Chiron BMP4, applied to whole or part aggregates, matrices, Matrigel, development these are unknown. Furthermore, different methods, matrices has yet be directly compared under same conditions. In this paper, we used a mouse embryonic stem cell aggregate model compare BMP4 pulses (applied aggregate) centers embedded them low‐percentage Matrigel. Each factor had effects on morphology, Brachyury protein expression, lineage commitment, having pulses. pulse was shown drive neural differentiation, while resulted better recapitulation aspects anterior–posterior axis formation, polarization expression anterior posterior genes observed. This further elucidates inform decisions around experimental conditions for vitro development.

Language: Английский

Citations

0