S‐Nitrosylation of NOTCH1 Regulates Mesenchymal Stem Cells Differentiation Into Hepatocyte‐Like Cells by Inhibiting Notch Signalling Pathway DOI Creative Commons

Xuesong Wang,

Yan Xu, Yue Wang

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(23)

Published: Dec. 1, 2024

The differentiation of mesenchymal stem cells (MSCs) into hepatocyte-like (HLCs) is considered one the most promising strategies for alternative hepatocyte transplantation to treat end-stage liver disease. To advance this method, it crucial gain a deeper understanding mechanisms governing hepatogenic differentiation. study demonstrated that suppression intracellular domain release Notch pathway receptor via γ-secretase inhibitor N-[(3, 5-difluorophenyl)acetyl]-L-alanyl-2-phenylglycine-1, 1-dimethylethyl ester (DAPT) significantly promotes expression hepatocyte-related genes and proteins in HLCs. Increased inducible NO synthase (iNOS) during led elevated endogenous production. Biotin switch assays revealed gradual increase S-nitrosylation (SNO)-NOTCH1 decrease overall NOTCH1 addition exogenous donor S-nitrosoglutathione (GSNO) SNO dithiothreitol (DTT) further SNO-NOTCH1 MSCs mature hepatocytes. Briefly, our results fully modification extracellular by NO, leading formation SNO-NOTCH1, inhibiting signalling pathway. Our highlights critical role regulating offers new insights driving process.

Language: Английский

Reprogramming of DPSC to Induced Pluripotent Stem Cells DOI
Sibel Yıldırım

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 157 - 183

Published: Jan. 1, 2024

Language: Английский

Citations

0
S‐Nitrosylation of NOTCH1 Regulates Mesenchymal Stem Cells Differentiation Into Hepatocyte‐Like Cells by Inhibiting Notch Signalling Pathway DOI Creative Commons

Xuesong Wang,

Yan Xu, Yue Wang

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(23)

Published: Dec. 1, 2024

The differentiation of mesenchymal stem cells (MSCs) into hepatocyte-like (HLCs) is considered one the most promising strategies for alternative hepatocyte transplantation to treat end-stage liver disease. To advance this method, it crucial gain a deeper understanding mechanisms governing hepatogenic differentiation. study demonstrated that suppression intracellular domain release Notch pathway receptor via γ-secretase inhibitor N-[(3, 5-difluorophenyl)acetyl]-L-alanyl-2-phenylglycine-1, 1-dimethylethyl ester (DAPT) significantly promotes expression hepatocyte-related genes and proteins in HLCs. Increased inducible NO synthase (iNOS) during led elevated endogenous production. Biotin switch assays revealed gradual increase S-nitrosylation (SNO)-NOTCH1 decrease overall NOTCH1 addition exogenous donor S-nitrosoglutathione (GSNO) SNO dithiothreitol (DTT) further SNO-NOTCH1 MSCs mature hepatocytes. Briefly, our results fully modification extracellular by NO, leading formation SNO-NOTCH1, inhibiting signalling pathway. Our highlights critical role regulating offers new insights driving process.

Language: Английский

Citations

0